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GIT2 (NM_057169) Human Untagged Clone
CAT＃: SC124350
GIT2 (untagged)-Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 1
CNY 5,568.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAT-2; CAT2; PKL |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_057169 edited
ATGTCGAAACGGCTCCGGAGCAGCGAGGTGTGCGCTGACTGCAGCGGGCCGGATCCTTCC TGGGCATCAGTAAATAGGGGAACGTTTTTATGTGATGAGTGCTGCAGTGTCCATCGGAGT CTAGGGCGCCATATCTCCCAAGTGAGGCATCTGAAACACACACCGTGGCCTCCAACACTG CTTCAGATGGTTGAGACCTTGTATAATAACGGTGCTAACTCTATATGGGAGCATTCTTTG CTGGACCCTGCGTCTATTATGAGTGGAAGACGTAAAGCTAATCCACAGGATAAAGTACAT CCCAATAAAGCGGAATTCATCAGAGCCAAGTATCAGATGTTAGCGTTCGTCCATCGCTTG CCCTGCCGGGATGACGATAGTGTGACTGCCAAAGATCTTAGCAAGCAACTCCATTCGAGC GTGAGAACAGGGAATCTTGAAACCTGTTTGAGACTGTTATCTTTAGGAGCACAAGCCAAC TTCTTTCATCCTGAAAAAGGAAACACCCCACTCCATGTTGCCTCCAAAGCAGGGCAGATT TTACAGGCTGAATTATTGGCAGTATATGGAGCAGACCCAGGCACACAGGATTCTAGTGGG AAAACTCCCGTTGATTATGCAAGGCAAGGAGGGCACCATGAGCTGGCAGAGCGCCTCGTG GAAATACAGTATGAGCTAACGGACAGACTAGCCTTCTATCTCTGTGGCAGGAAACCAGAT CACAAAAATGGACAGCACTTTATAATACCTCAAATGGCAGACAGACGGCTCAGCCTGGAT TTGTCTGAATTGGCAAAAGCTGCTAAGAAGAAACTTCAATCTCTAAGTAATCATTTGTTT GAAGAACTTGCCATGGATGTGTACGATGAAGTTGACAGGCGAGAGACGGATGCAGTCTGG CTTGCCACGCAAAACCACAGCGCCCTGGTAACCGAGACAACGGTCGTCCCCTTTCTTCCG GTCAATCCTGAGTACTCATCAACACGAAATCAGGGCAGACAGAAGTTAGCTCGGTTCAAC GCCCATGAGTTTGCCACGCTGGTCATTGACATTCTCAGTGACGCCAAGAGGAGACAGCAG GGCAGTTCTCTCTCGGGTTCAAAAGACAATGTGGAGCTCATACTGAAAACCATCAATAAC CAGCACAGCGTTGAGAGTCAAGACAACGATCAGCCCGACTATGACAGCGTGGCATCAGAC GAAGACACAGATTTGGAAACCACTGCAAGCAAAACAAACCGGCAGAAGAGCCTAGATTCA GATTTATCAGATGGACCAGTCACTGTACAGGAATTTATGGAGGTCAAAAACGCTCTAGTG GCTTCTGAGGCCAAGATACAGCAGCTAATGAAGGTGAATAACAACTTGAGTGACGAGCTG AGAATTATGCAGAAAAAGCTTCAAACACTCCAGAGTGAAAATTCGAACCTCAGGAAACAG GCCACAACCAATGTATATCAGGTGCAAACTGGTTCTGAGTACACAGACACTTCCAACCAC TCTTCCTTAAAGAGACGTCCGTCTGCCCGGGGCAGTAGGCCCATGTCCATGTACGAGACC GGATCAGGTCAGAAACCATATCTCCCAATGGGAGAAGCGAGCCGCCCCGAAGAGAGCAGG ATGAGACTCCAGCCCTTCCCCGCGCACGCATCCAGGCTGGAGAAGCAGAACAGCACACCT GAGAGTGACTACGACAACACTCCCAACGACATGGAGCCAGATGGCATGGGGTCAAGCCGA AAGGGACGGCAAAGAAGTATGGTGTGGCCAGGGGATGGCTTGGTACCAGACACAGCAGAA CCCCATGTGGCCCCAAGCCCCACTCTCCCTAGCACCGAAGATGTCATCAGGAAGACTGAA CAGATCACCAAAAACATACAGGAGCTCTTAAGAGCAGCCCAAGAAAATAAACATGACAGA AACCCAAGTCTGATATGGTGAGGACTTCCCTTCGTTTACTGACGTCCAGTGCCTACCGAC TGCAGTCAGAGTGCAAGAAGACCCTCCCAGGGGACCCCGGCTCACCCACAGACGTTCAGC TGGTCACGCAGCAGGTCATCCAGTGTGCGTACGACATCGCCAAGGCTGCCAAGCAGCTGG TTACCATCACCACCAAAGAGAACAACAACTGA >OriGene 5' read for NM_057169 unedited
TTGTACACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGTCAGCGCCGCCG CAGCTGGGACCCGTTAGAGCGGAAGCGCCGCCGCCACCGCCGCCTTTGCTGTCCCCCGGC CTCTAGTTCCCCGCAGGTGGGAGGTGGGAGCCATGTCGAAACGGCTCCGGAGCAGCGAGG TGTGCGCTGACTGCAGCGGGCCGGATCCTTCCTGGGCATCAGTAAATAGGGGAACGTTTT TATGTGATGAGTGCTGCAGTGTCCATCGGAGTCTAGGGCGCCATATCTCCCAAGTGAGGC ATCTGAAACACACACCGTGGCCTCCAACACTGCTTCAGATGGTTGAGACCTTGTATAATA ACGGTGCTAACTCTATATGGGAGCATTCTTTGCTGGACCCTGCGTCTATTATGAGTGGAA GACGTAAAGCTAATCCACAGGATAAAGTACATCCCAATAAAGCGGAATTCATCAGAGCCA AGTATCAGATGTTAGCGTTCGTCCATCGCTTGCCCTGCCGGGATGACGATAGTGTGACTG CCAAAGATCTTAGCAAGCAACTCCATTCGAGCGTGAGAACAGGGAATCTTGAAACCTGTT TGAGACTGTTATCTTTAGGAGCACAAGCCAACTTCTTTCATCCTGAAAAAGGAAACACCC CACTCCATGTTGCCTCCAAAGCAGGGCAGATTNTACAGGCTGAATTATTGGCAGTATATG GAGCAGACCCAGGCACACAGGATTCTAGTGGGAAAACTCCCGTTGATTATGCANGGGCAG GGAGGCACCATGAGCTGGCAGAGCGCCTCGTGGAATACAGTATGAGCTAACGGACAGACT AGCCTTCTATCTCTGTGGCAGGANACCAGATCACANAATGGACAGCACTTTNATATC >OriGene 3' read for NM_057169 unedited
AATTTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTGTTTTATGAAAATATTTCATTTATAG TTTCAGCCATAGGCAGGGAGTTTTAAGGGATGGAAAAGTTTTCACAAACTTTAGACAATG AAACTATACTTTTGGATGTATGTGATCAACTCAACAGTTGTTGACAACACAACCGAACAT CAGAAACTAAACCAGCAAATAGGCACAAAATAAGGCAAGTGGGTTAAATAGTTGAAAATT GGTTAGAAAACATGCAAAAATAATACTGAGTTTTCTTTTAAAAAGTTTTAAACCGTCATT AAATGTTTCTTTTTGTAGAAATCTGAAGAGTTCTGCGTCTGAATTTGAAATTGGACTTTA TAAAGCCTAGAAAACAGAGGAGGCGGTGCCCTGCCCTTGTCAGTTGTTGTTCTCTTTGGT GGTGATGGTAACCAGCTGCTTGGCAGCCTTGGCGATGTCGTACGCACACTGGATGACCTG CTGCGTGACCAGCTGAACGTCTGTGGGTGAGCCGGGGTCCCCTGGGAGGGTCTTCTTGCA CTCTGACTGCAGTCGGTAGGCACTGGACGTCAGTAAACGAAGGGAAGTCCTCACCATATC AGACTTGGGTTTCTGTCATGTTTATTNTCTTGGGCTGCTCCTAAGAGCTCCTGTATGTTT TTGGTGATCTGTTCAGTCTTCCTGATGACATCCTTCGTGCCTAGGAAAAGTGGGCTTGGG GCCCCATGGGGTTCTGCTGTGTTTGTTACCAGCCATCCCCTGGCCAACCATACTTTTTGN CGCCCTTTCCGGTTGACCCATGCCATTTGCCTCATGTCGTGGGAATGTTGCCTAACACCT CTCAGGTGCCTGTCTGTTCTCCACCTGTATCCTTCCCCGGAAAGCTCGANTCTATCCTGG TTTTTCGGGGGGGTGTTTTTCCATTGGAAGAAAGGTTTTGACCTATCCGCTCTTAACTGC AAGGGCCACTGCCCCGCCAAAAGAGTTTTAAGAAAAAGGTGAAGGCCGGTTCCAAAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_057169 |
| Insert Size | 2820 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_057169.2, NP_476510.1 |
| RefSeq Size | 5525 bp |
| RefSeq ORF | 2280 bp |
| Locus ID | 9815 |
| UniProt ID | Q14161 |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis |
| Gene Summary | This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq, Sep 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212976 | GIT2 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 1 |
CNY 7,600.00 |
|
| RC212976L3 | Lenti-ORF clone of GIT2 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 1 |
CNY 9,500.00 |
|
| RC212976L4 | Lenti-ORF clone of GIT2 (mGFP-tagged)-Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 1 |
CNY 9,500.00 |
|
| RG212976 | GIT2 (tGFP-tagged) - Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 1 |
CNY 8,360.00 |
