FBXO21 (NM_033624) Human Untagged Clone
CAT#: SC124348
FBXO21 (untagged)-Human F-box protein 21 (FBXO21), transcript variant 1
CNY 4,680.00
Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | FBX21 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for NM_033624 edited
GCGGCCCCAGGTACGCGGACAAGATGGCGGCGGCAGCAGTCGACAGCGCGATGGAGGTGG TGCCGGCGCTGGCGGAGGAGGCCGCGCCGGAGGTAGCGGGCCTCAGCTGCCTCGTCAACC TGCCGGGTGAGGTGCTGGAGTACATCCTGTGCTGCGGCTCGCTGACGGCCGCCGACATCG GCCGTGTCTCCAGCACCTGCCGGCGGCTGCGCGAGCTGTGCCAGAGCAGCGGGAAGGTGT GGAAGGAGCAGTTCCGGGTGAGGTGGCCTTCCCTTATGAAACACTACAGCCCCACCGACT ACGTCAATTGGTTGGAAGAGTATAAAGTTCGGCAAAAAGCTGGGTTAGAAGCGCGGAAGA TTGTAGCCTCGTTCTCAAAGAGGTTCTTTTCAGAGCACGTTCCTTGTAATGGCTTCAGTG ACATTGAGAACCTTGAAGGACCAGAGATTTTTTTTGAGGATGAACTGGTGTGTATCCTAA ATATGGAAGGAAGAAAAGCTTTGACCTGGAAATACTACGCAAAAAAAATTCTTTACTACC TGCGGCAACAGAAGATCTTAAATAATCTTAAGGCCTTTCTTCAGCAGCCAGATGACTATG AGTCGTATCTTGAAGGTGCTGTATATATTGACCAGTACTGCAATCCTCTCTCCGACATCA GCCTCAAAGACATCCAGGCCCAAATTGACAGCATCGTGGAGCTTGTTTGCAAAACCCTTC GGGGCATAAACAGTCGCCACCCCAGCTTGGCCTTCAAGGCAGGTGAATCATCCATGATAA TGGAAATAGAACTCCAGAGCCAGGTGCTGGATGCCATGAACTATGTCCTTTACGACCAAC TGAAGTTCAAGGGGAATCGAATGGATTACTATAATGCCCTCAACTTATATATGCATCAGG TTTTGATTCGCAGAACAGGAATCCCAATCAGCATGTCTCTGCTCTATTTGACAATTGCTC GGCAGTTGGGAGTCCCACTGGAGCCTGTCAACTTCCCAAGTCACTTCTTATTAAGGTGGT GCCAAGGCGCAGAAGGGGCGACCCTGGACATCTTTGACTACATCTACATAGATGCTTTTG GGAAAGGCAAGCAGCTGACAGTGAAAGAATGCGAGTACTTGATCGGCCAGCACGTGACTG CAGCACTGTATGGGGTGGTCAATGTCAAGAAGGTGTTACAGAGAATGGTGGGAAACCTGT TAAGCCTGGGGAAGCGGGAAGGCATCGACCAGTCATACCAGCTCCTGAGAGACTCGCTGG ATCTCTATCTGGCAATGTACCCGGACCAGGTGCAGCTTCTCCTCCTCCAAGCCAGGCTTT ACTTCCACCTGGGAATCTGGCCAGAGAAGTCTTTCTGTCTTGTTTTGAAGGTGCTTGACA TCCTCCAGCACATCCAAACCCTAGACCCGGGGCAGCACGGGGCGGTGGGCTACCTGGTGC AGCACACTCTAGAGCACATTGAGCGCAAAAAGGAGGAGGTGGGCGTAGAGGTGAAGCTGC GCTCCGATGAGAAGCACAGAGATGTCTGCTACTCCATCGGGCTCATTATGAAGCATAAGA GGTATGGCTATAACTGTGTGATCTACGGCTGGGACCCCACCTGCATGATGGGACACGAGT GGATCCGGAACATGAACGTCCACAGCCTGCCGCACGGCCACCACCAGCCTTTCTATAACG TGCTGGTGGAGGACGGCTCCTGTCGATACGCAGCCCAAGAAAACTTGGAATATAACGTGG AGCCTCAAGAAATCTCACACCCTGACGTGGGACGCTATTTCTCAGAGTTTACTGGCACTC ACTACATCCCAAACGCAGAGCTGGAGATCCGGTATCCAGAAGATCTGGAGTTTGTCTATG AAACGGTGCAGAATATTTACAGTGCAAAGAAAGAGAACATAGATGAGTAAAGTCTAGAGA GGACATTGCACCTTTGCTGCTGCTGCTATCTTCCAAGAGAACGGGACTCCGGAAGAAGAC GTCTCCACGGAGCCCTCGGGACCTGCTGCACCAGGAAAGCCACTCCACCAGTAGTGCTGG TTGCCTCCTACTAAGTTTAAATACCGTGTGCTCTTCCCCAGCTGCAAAGACAATGTTGCT CTCCGCCTACACTAGTGAATTAATCTGAAAGGCACTGTGTCAGTGGCATGGCTTGTATGC TTGTCCTGTGGTGACAGTTTGTGACATTCTGTCTTCATGAGGTCTCACAGTCGACGCTCC TGTAATCATTCTTTGTATTCACTCCATTCCCCTGTCTGTCTGCATTTGTCTCAGAACATT TCCTTGGCTGGACAGATGGGGTTATGCATTTGCAATAATTTCCTTCTGATTTCTCTGTGG AACGTGTTCGGTCCCGAGTGAGGACTGTGTGTCTTTTTACCCTGAAGTTAGTTGCATATT CAGAGGTAAAGTTGTGTGCTATCTTGGCAGCATCTTAGAGATGGAGACATTAACAAGCTA ATGGTAATTAGAATCATTTGAATTTATTTTTTTCTAATATGTGAAACACAGATTTCAAGT GTTTTATCTTTTTTTTTTAAATTTAAATGGGAATATAACACAGTTTTCCCTTCCATATTC CTCTCTTGAGTTTATGCACATCTCTATAAATCATTAGTTTTCTATTTTATTACATAAAAT TCTTTTAGAAAATGCAAATAGTGAACTTTGTGAATGGATTTTTCCATACTCATCTACAAT TCCTCCATTTTAAATGACTACTTTTATTTTTTAATTTAAAAAATCTACTTCAGTATCATG AGTAGGTCTTACATCAGTGATGGGTTCTTTTTGTAGTGAGACATACAAATCTGATGTTAA TGTTTGCTCTTAGAAGTCATACTCCATGGTCTTCAAAGACCAAAAAAATGAGGTTTTGCT TTTGTAATCAGGAAAAAAAAAAATTAATGAACCTTAAAAAAAAAAAAAAA |
Restriction Sites | NotI-NotI |
ACCN | NM_033624 |
Insert Size | 2900 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to NM_033624.2. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_033624.2, NP_296373.1 |
RefSeq Size | 4248 bp |
RefSeq ORF | 1887 bp |
Locus ID | 23014 |
UniProt ID | O94952 |
Domains | F-box |
Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) contains an additional 21 nt fragment within the coding region, and thus encodes a 7 aa longer isoform than variant 2. |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Peptidic degron in EID1 is recognized by an SCF E3 ligase complex containing the orphan F-box protein FBXO21
,Zhang, C;Li, X;Adelmant, G;Dobbins, J;Geisen, C;Oser, MG;Wucherpfenning, KW;Marto, JA;Kaelin, WG;,
Proc. Natl. Acad. Sci. U.S.A.
,PubMed ID 26631746
[FBXO21]
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Other Versions
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RC223095 | FBXO21 (Myc-DDK-tagged)-Human F-box protein 21 (FBXO21), transcript variant 1 |
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RC223095L3 | Lenti-ORF clone of FBXO21 (Myc-DDK-tagged)-Human F-box protein 21 (FBXO21), transcript variant 1 |
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RC223095L4 | Lenti-ORF clone of FBXO21 (mGFP-tagged)-Human F-box protein 21 (FBXO21), transcript variant 1 |
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RG223095 | FBXO21 (tGFP-tagged) - Human F-box protein 21 (FBXO21), transcript variant 1 |
CNY 6,080.00 |