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IDH3G (NM_174869) Human Untagged Clone
CAT＃: SC124266
IDH3G (untagged)-Human isocitrate dehydrogenase 3 (NAD+) gamma (IDH3G), nuclear gene encoding mitochondrial protein, transcript variant 2
CNY 3,656.00
CNY 6,180.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | H-IDHG |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_174869, the custom clone sequence may differ by one or more nucleotides
ATGGCGCTGAAGGTAGCGACCGTCGCCGGCAGCGCCGCGAAGGCGGTGCTCGGGCCAGCCCTTCTCTGCC GTCCCTGGGAGGTTCTAGGCGCCCACGAGGTCCCCTCGAGGAACATCTTTTCAGAACAAACAATTCCTCC GTCCGCTAAGTATGGCGGGCGGCACACGGTGACCATGATCCCAGGGGATGGCATCGGGCCAGAGCTCATG CTGCATGTCAAGTCCGTCTTCAGGCACGCATGTGTACCAGTGGACTTTGAAGAGGTGCACGTGAGTTCCA ATGCTGATGAAGAGGACATTCGCAATGCCATCATGGCCATCCGCCGGAACCGCGTGGCCCTGAAGGGCAA CATCGAAACCAACCATAACCTGCCACCGTCGCACAAATCTCGAAACAACATCCTTCGCACCAGCCTGGAC CTCTATGCCAACGTCATCCACTGTAAGAGCCTTCCAGGCGTGGTGACCCGGCACAAGGACATAGACATCC TCATTGTCCGGGAGAACACAGAGGGCGAGTACAGCAGCCTGGAGCATGAGAGTGTGGCGGGAGTGGTGGA GAGCCTGAAGATCATCACCAAGGCCAAGTCCCTGCGCATTGCCGAGTATGCCTTCAAGCTGGCGCAGGAG AGCGGGCGCAAGAAAGTGACGGCCGTGCACAAGGCCAACATCATGAAACTGGGCGATGGGCTTTTCCTCC AGTGCTGCAGGGAGGTGGCAGCCCGCTACCCTCAGATCACCTTCGAGAACATGATTGTGGATAACACCAC CATGCAGCTGGTGTCCCGGCCCCAGCAGTTTGATGTCATGGTGATGCCCAATCTCTATGGCAACATCGTC AACAATGTCTGCGCGGGACTGGTCGGGGGCCCAGGCCTTGTGGCTGGGGCCAACTATGGCCATGTGTACG CGGTGTTTGAAACAGCTACGAGGAACACCGGCAAGAGTATCGCCAATAAGAACATCGCCAACCCCACGGC CACCCTGCTGGCCAGCTGCATGATGCTGGACCACCTCAAGCTGCACTCCTATGCCACCTCCATCCGTAAG GCTGTCCTGGCATCCATGGACAATGAGAATGTGAGGTTCCCCTCGCACCCTACCCTGCTGCCCCGCCCAG TCTCCCCCTGCAGCCTCCTGTAG >OriGene 5' read for NM_174869 unedited
GGGGGGNNNNNNNNNNNNNNNCNNCCNNNCNNCNNNCGGTTCAGAATTGTATACGATCAT ATAGGCGGCCGCGNAATTCGCACCAGGTCGGGCCGTGCGGAGGCTCTCACTTTCCGTCTG GCGCTGAAGGTAGCGACCGTCGCCGGCAGCGCCGCGAAGGCGGTGCTCGGGCCAGCCCTT CTCTGCCGTCCCTGGGAGGTTCTAGGCGCCCACGAGGTCCCCTCGAGGAACATCTTTTCA GAACAAACAATTCCTCCGTCCGCTAAGTATGGCGGGCGGCACACGGTGACCATGATCCCA GGGGATGGCATCGGGCCAGAGCTCATGCTGCATGTCAAGTCCGTCTTCAGGCACGCATGT GTACCAGTGGACTTTGAAGAGGTGCACGTGAGTTCCAATGCTGATGAAGAGGACATTCGC AATGCCATCATGGCCATCCGCCGGAACCGCGTGGCCCTGAAGGGCAACATCGAAACCAAC CATAACCTGCCACCGTCGCACAAATCTCGAAACAACATCCTTCGCACCAGCCTGGACCTC TATGCCAACGTCATCCACTGTAAGAGCCTTCCAGGCGTGGTGACCCGGCACAAGGACATA GACATCCTCATTGTCCGGGAGAACACAGAGGGCGAGTACAGCAGCCTGGAGCATGAAAGT GNTGCGGGAGTGGTGGAAGCCTGAAGACATCACCAAGCCAAGTCCCTGCGCATTGCCGAG TATGCCTTNCAGNNCTGCGCAGAGAAGCGGCGCAAGAAAGTGACNGCCGTGCACAGGNCC ACATCATGAAACTGGGCGATGGGCTTTCCTCCAGTGCTGCAGGNNAGTGGCANCCNGCTA CCCNAGATCACCTTCAGACATGATTGTGATACAACACATGCAGCTGGTGTCCGGCCCCAG CAGTTGAGTCATGGGATGCCCATCTCTATGCACATCGTCACATGTCTGCGGAACTGGTCG GGGCCCAGGCCTGGGGCTGGGGCAACTTGGCATGTGTACCCGTGTTTGAACA >OriGene 3' read for NM_174869 unedited
NCGTATACTATGNACGCGGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTTTTTAG GGAGAAGGTGCTTTATTCTGGGATCTGCGTACCAAGCTGGCTGGGGTGCTGGAGTGGGAA GGGGAATCCAAGGAGCAAACCAAGAAGGTCCTAGGGCCAGCCTAGGCCTCCACGGCCCGG CCGTTGATGACGCGGATGTGGCGGATGACGTCCTGGATGGCTTCAGATGTTGTGCCCTGG CCCCCGATGTCCGGAGTGTGCATCTGTAGGACACAGGCAGGCTCGGCACACACCAGGCCC TGCCACCCCCCGCTGTCTCCTGTGACGTGCAGGACTGGGATTGCCACAGGAGGGAAGTGG CACCAGCTAAGGGCCAGAAGATGGGGCCAGATCCAGTACCATCCTCCCCTGGGGGCTGTG GTCAGTGCATGAGGCGGGCTACAGGAGGCTGCAGGGGGAGACTGGGCGGGGCAGCAGGGT AGGGTGCGAGGGGAACCTCACATTCTCATTGTCCATGGATGCCAGGACAGCCTTACGGAT GGAGGTGGCATATGAGTGCAGCTTGAGGTGGTCCAGCATCATGCAGCTGGCCAGCAGGGT GGCCGTGGGGTTGGCGATGTTCTTATTGGCGATACTCTTGCCGGTGTTCCTCGTAGCTGT TTCAAACACCGCGTACACATGGCCATAGTTGGCCCCAGCCACAAGGCCTGGGCCCCCGAC CAGTCCCGCGCAGACATTGTTGACGATGTTGCCATAGAGATTGGGCATCACCATGACATC AAACTGCTGGGGCCGGGACACCANCTGCATGGTGGTGGTATCCACAATCATGTTCTCGAA GGTGATCTTGAGGTANCGGGCTGCCACCTTCCTGCAGCACTGGGAGAAAAGCCATG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_174869 |
| Insert Size | 1650 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_174869.1, NP_777358.1 |
| RefSeq Size | 1723 bp |
| RefSeq ORF | 1143 bp |
| Locus ID | 3421 |
| UniProt ID | P51553 |
| Protein Pathways | Citrate cycle (TCA cycle), Metabolic pathways |
| Gene Summary | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) differs in the 3' UTR and coding region resulting in a frameshift, compared to variant 1. Variant 2 encodes a shorter isoform (b) with a distinct C-terminus, compared to isoform a. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218514 | IDH3G (Myc-DDK-tagged)-Human isocitrate dehydrogenase 3 (NAD+) gamma (IDH3G), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 3,656.00 |
|
| RC218514L3 | Lenti ORF clone of Human isocitrate dehydrogenase 3 (NAD+) gamma (IDH3G), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218514L4 | Lenti ORF clone of Human isocitrate dehydrogenase 3 (NAD+) gamma (IDH3G), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG218514 | IDH3G (tGFP-tagged) - Human isocitrate dehydrogenase 3 (NAD+) gamma (IDH3G), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 5,256.00 |
