PAM (NM_138822) Human Untagged Clone
CAT#: SC124251
PAM (untagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 4
CNY 6,504.00
Product images
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | PAL; PHM |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_138822, the custom clone sequence may differ by one or more nucleotides
ATGGCTGGCCGCGTCCCTAGCCTGCTAGTTCTCCTTGTTTTTCCAAGCAGCTGTTTGGCTTTCCGAAGCC CACTTTCTGTCTTTAAGAGGTTTAAAGAAACTACCAGACCATTTTCCAATGAATGTCTTGGTACCACCAG ACCCGTAGTTCCTATTGATTCATCAGATTTTGCATTGGATATTCGCATGCCTGGGGTTACACCTAAACAG TCCGATACATACTTCTGCATGTCTATGCGAATACCAGTGGATGAGGAAGCCTTCGTGATTGACTTCAAGC CTCGAGCCAGCATGGATACTGTCCATCACATGTTACTTTTTGGATGCAATATGCCTTCATCCACTGGAAG TTACTGGTTTTGTGATGAAGGAACCTGTACAGATAAAGCCAATATTCTGTATGCCTGGGCGAGAAATGCT CCCCCTACCCGGCTCCCCAAAGGTGTTGGATTCAGAGTTGGAGGAGAGACTGGAAGTAAATACTTTGTAC TACAGGTACACTATGGGGATATTAGTGCTTTTAGAGATAATAACAAGGACTGTTCTGGTGTGTCCTTACA CCTCACACGTCTGCCACAGCCTTTAATTGCTGGCATGTACCTTATGATGTCTGTTGACACTGTTATCCCA GCAGGAGAAAAAGTGGTGAATTCTGACATTTCATGCCATTATAAAAATTATCCAATGCATGTCTTTGCCT ATAGAGTTCACACTCACCATTTAGGTAAGGTAGTAAGTGGATACAGAGTAAGAAATGGACAGTGGACACT GATTGGACGGCAGAGCCCTCAGCTGCCACAGGCTTTCTACCCTGTGGGGCATCCAGTTGATGTAAGTTTT GGTGACCTACTGGCTGCAAGATGTGTATTCACTGGTGAAGGAAGGACAGAAGCCACACACATTGGTGGCA CGTCTAGTGATGAAATGTGCAACTTATACATTATGTATTACATGGAAGCCAAGCATGCAGTTTCTTTCAT GACCTGTACCCAGAATGTAGCTCCAGATATGTTCAGAACCATACCACCAGAGGCCAACATTCCAATTCCC GTGAAGTCTGATATGGTTATGATGCATGAACATCATAAAGAAACAGAATATAAAGATAAGATTCCTTTAC TACAGCAGCCAAAACGAGAAGAAGAAGAAGTGTTAGACCAGGGTGATTTCTATTCACTACTTTCCAAGCT GCTAGGAGAAAGGGAAGATGTTGTTCATGTGCACAAATATAATCCTACAGAAAAGGCAGAATCAGAGTCA GACCTGGTAGCTGAGATTGCAAATGTAGTCCAAAAAAAGGATCTTGGTCGATCTGATGCCAGAGAGGGTG CAGAACATGAGAGGGGTAATGCTATTCTTGTCAGAGACAGAATTCACAAATTCCACAGACTAGTATCTAC CTTGAGGCCACCAGAGAGCAGAGTTTTCTCATTACAGCAGCCCCCACCTGGTGAAGGCACCTGGGAACCA GAACACACAGGAGATTTCCACATGGAAGAGGCACTGGATTGGCCTGGAGTATACTTGTTACCAGGCCAGG TTTCTGGGGTGGCTCTAGACCCTAAGAATAACCTGGTGATTTTCCACAGAGGTGACCATGTCTGGGATGG AAACTCGTTTGACAGCAAGTTTGTTTACCAGCAAATAGGACTCGGACCAATTGAAGAAGACACTATTCTT GTCATAGATCCAAATAATGCTGCAGTACTCCAGTCCAGTGGAAAAAATCTGTTTTACTTGCCACATGGCT TGAGTATAGATAAAGATGGGAATTATTGGGTCACAGACGTGGCTCTCCATCAGGTGTTCAAACTGGATCC AAACAATAAAGAAGGCCCTGTATTAATCCTGGGAAGGAGCATGCAACCAGGCAGTGACCAGAATCACTTC TGTCAACCCACTGATGTGGCTGTGGATCCAGGCACTGGAGCCATTTATGTATCAGATGGTTACTGCAACA GCAGGATTGTGCAGTTTTCACCAAGTGGAAAGTTCATCACACAGTGGGGAGAAGAGTCTTCAGGGAGCAG TCCTCTGCCAGGCCAGTTCACTGTTCCTCACAGCTTGGCTCTTGTGCCTCTTTTGGGCCAATTATGTGTG GCAGACCGGGAAAATGGTCGGATCCAGTGTTTTAAAACTGACACCAAAGAATTTGTGAGAGAGATTAAGC ATTCATCATTTGGAAGAAATGTATTTGCAATTTCATATATACCAGGCTTGCTCTTTGCAGTGAATGGGAA GCCTCATTTTGGGGACCAAGAACCTGTACAAGGATTTGTGATGAACTTTTCCAATGGGGAAATTATAGAC ATCTTCAAGCCAGTGCGCAAGCACTTTGATATGCCTCATGATATTGTTGCATCTGAAGATGGGACTGTGT ACATTGGAGATGCTCATACCAACACCGTGTGGAAGTTCACCTTGACTGAGAAATTGGAACATCGATCAGT TAAAAAGGCTGGCATTGAGGTCCAGGAAATCAAAGGAAAGGGAAGTGGAGGCTTAAACCTTGGTAATTTC TTTGCAAGCCGTAAGGGCTACAGTCGAAAAGGGTTTGACCGGCTTAGCACTGAGGGCAGTGACCAAGAGA AAGAGGATGATGGAAGTGAATCAGAAGAGGAGTATTCAGCACCTCTGCCTGCGCTCGCACCTTCCTCCTC CTGA >OriGene 5' read for NM_138822 unedited
GTCAGCATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACCAGCCGTGGTCGCG CAGGTTCTGAATGATGACTGACGCGGGTTTGGGTGATACCCCTCACAGCCCCTGTCATTC CGGAGTGATAAGGCACCCGCGCGTCTAGCCCCAGCGCCAGGGCACGCGAGCGGCGCTGGA GGGAGGAAAGCTTCCGCCTGCGGGCCGGACAAAAGTCCCGCCTGCCCACGGCTTTTTGCC CGCCGCTCGTGACCGAGACGCCTCGCCGCGGCCAGCTCGCTGCTCTCGCTGGCGGATGGT GTGTGGCCGCCGCAAGACGCCCGCCGTGCCCGGGCCATGAAGTAGCGGCTGCTGGCGGCG CCGCTGCCCAACCGCCAGCCCCAGCCCCGCGCTGCGCTGCCCGGTCCTCTCCCGGCGGGG TCGTATCGGCGTGGACATGGCTGGCCGCGTCCCTAGCCTGCTAGTTCTCCTTGTTTTTCC AAGCAGCTGTTTGGCTTTCCGAAGCCCACTTTCTGTCTTTAAGAGGTTTAAAGAAACTAC CAGACCATTTTCCAATGAATGTCTTGGTACCACCAGACCCGTAGTTCCTATTGATTCATC AGATTTTGCATTGGATATTCGCATGCCTGGGGTTACACCTAAACAGTCCGATACATACTT CTGCATGTCTATGCGAATACCAGTGGATGAGGAAGCCTTCGTGATTGACTTCAAGCCTCG AGCCAGCATGGATACTGTCCATCACATGTTACTTTNTGGATGCAATATGCCTTCATCCAC TGGAAGTTACTGGTTTTGTGATGAAAGAACCTGTACAGATAAAGCCAATATTCTGTATGC CTGGGCGATGAATGCTNCCCCTACCCCGCTCCCAAGTGTTGGATTCAAACTGGAGGAAGA CTGGAAGTAATACTTGTACTACAGTCACTATGGGNATATTATGCTTTAAGATATAACAGA CTGTNTGGGGTGTCTTACC |
Restriction Sites | NotI-NotI |
ACCN | NM_138822 |
Insert Size | 3950 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138822.1, NP_620177.1 |
RefSeq Size | 5098 bp |
RefSeq ORF | 2664 bp |
Locus ID | 5066 |
UniProt ID | P19021 |
Protein Families | Druggable Genome, Transmembrane |
Gene Summary | This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (4) lacks two alternate in-frame exons in the 3' coding region compared to variant 5. The encoded isoform (d) is shorter than isoform e. This isoform (d) may undergo proteolytic processing similar to isoform e. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216147 | PAM (Myc-DDK-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 4 |
CNY 10,392.00 |
|
RC216147L3 | Lenti-ORF clone of PAM (Myc-DDK-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 4 |
CNY 11,310.00 |
|
RC216147L4 | Lenti-ORF clone of PAM (mGFP-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 4 |
CNY 11,310.00 |
|
RG216147 | PAM (tGFP-tagged) - Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 4 |
CNY 10,360.00 |