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DGKG (NM_001346) Human Untagged Clone
CAT＃: SC124112
DGKG (untagged)-Human diacylglycerol kinase, gamma 90kDa (DGKG), transcript variant 1
CNY 5,800.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DAGK3; DGK-GAMMA |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001346, the custom clone sequence may differ by one or more nucleotides
ATGGGTGAAGAACGGTGGGTCTCCCTCACTCCAGAAGAATTTGACCAACTCCAGAAATATTCAGAATATT CCTCCAAGAAGATAAAAGATGCCTTGACTGAATTTAATGAGGGTGGGAGCCTCAAACAATATGACCCACA TGAGCCGATTAGCTATGATGTCTTCAAGCTGTTCATGAGGGCGTACCTGGAGGTGGACCTTCCCCAGCCA CTGAGCACTCACCTCTTCCTGGCCTTCAGCCAGAAGCCCAGACACGAGACCTCTGACCACCCGACGGAGG GAGCCAGCAACAGTGAGGCCAACAGCGCAGATACTAATATACAGAATGCAGATAATGCCACCAAAGCAGA CGAGGCCTGTGCCCCTGATACTGAATCAAATATGGCTGAGAAGCAAGCACCAGCTGAAGACCAAGTGGCT GCGACCCCCCTGGAACCCCCCGTCCCTCGGTCTTCAAGCTCGGAATCCCCAGTGGTATACCTGAAGGATG TTGTGTGCTACCTGTCCCTGCTGGAGACGGGGAGGCCTCAGGATAAGCTGGAGTTCATGTTTCGCCTCTA TGATTCAGATGAGAACGGTCTCCTGGACCAAGCGGAGATGGATTGCATTGTCAACCAAATGCTGCATATT GCCCAGTACCTGGAGTGGGATCCCACAGAGCTGAGGCCTATATTGAAGGAGATGCTGCAAGGGATGGACT ACGACCGGGACGGCTTTGTGTCTCTACAGGAATGGGTCCATGGAGGGATGACCACCATCCCATTGCTGGT CCTCCTGGGGATGGATGACTCTGGCTCCAAGGGGGATGGGCGGCACGCCTGGACCATGAAGCACTTCAAG AAACCAACCTACTGCAACTTCTGCCATATCATGCTCATGGGCGTCCGCAAGCAAGGCCTGTGCTGCACTT ACTGTAAATACACTGTCCACGAACGCTGTGTGTCCAGAAACATTCCTGGTTGTGTCAAAACGTACTCAAA AGCCAAAAGGAGTGGTGAGGTGATGCAGCACGCATGGGTGGAAGGGAACTCCTCCGTCAAGTGTGACCGG TGCCACAAAAGTATCAAGTGCTACCAGAGTGTCACCGCGCGGCACTGCGTGTGGTGCCGGATGACGTTTC ACCGCAAATGTGAATTATCAACGTTGTGTGACGGTGGGGAACTCAGAGACCACATCTTACTGCCCACCTC CATATGCCCCATCACCCGGGACAGGCCAGGTGAGAAGTCTGATGGCTGCGTGTCCGCCAAGGGCGAACTT GTCATGCAGTATAAGATCATCCCCACCCCGGGTACCCACCCCCTGCTGGTCTTGGTGAACCCCAAGAGTG GAGGGAGACAAGGAGAAAGAATTCTTCGGAAATTCCACTATCTGCTCAACCCCAAACAAGTTTTCAACCT GGACAATGGGGGGCCTACTCCAGGGTTGAACTTTTTCCGTGATACTCCAGACTTCCGTGTTTTGGCCTGT GGTGGAGATGGGACAGTTGGCTGGATTTTGGATTGCATTGATAAGGCCAACTTTGCAAAGCATCCACCAG TGGCTGTCCTGCCTCTTGGAACAGGAAATGACCTTGCCCGTTGTCTCCGCTGGGGAGGAGGTTATGAAGG GGGCAGCTTGACAAAAATCCTGAAAGACATTGAGCAGAGCCCCTTGGTGATGCTGGACCGCTGGCATCTG GAAGTCATCCCCAGAGAGGAAGTGGAAAACGGGGACCAGGTCCCATACAGCATCATGAACAACTATTTCT CCATTGGTGTGGACGCTTCCATTGCACACAGATTCCATGTGATGAGAGAGAAACATCCTGAAAAATTCAA CAGCAGGATGAAGAACAAGCTGTGGTACTTTGAATTTGGCACCTCGGAGACTTTTGCAGCGACCTGCAAG AAACTCCACGACCACATTGAGTTGGAGTGTGATGGGGTTGGGGTGGACCTGAGCAACATCTTCCTGGAAG GCATTGCCATTCTCAACATTCCCAGCATGTACGGAGGCACCAATCTCTGGGGAGAAAACAAGAAGAACCG GGCTGTGATCCGGGAAAGCAGGAAGGGTGTCACTGACCCCAAAGAACTGAAATTCTGCGTTCAAGACCTC AGTGACCAGCTCCTTGAAGTGGTGGGGCTAGAAGGAGCCATGGAGATGGGGCAGATCTACACCGGCCTGA AGAGTGCAGGCAGGAGGCTGGCCCAGTGCGCCTCTGTCACCATCAGGACAAACAAGCTGCTGCCAATGCA AGTGGATGGAGAACCCTGGATGCAGCCATGTTGCACGATTAAAATTACTCACAAGAACCAAGCGCCCATG ATGATGGGGCCTCCCCAGAAGAGCAGCTTCTTCTCGTTGAGAAGGAAGAGCCGTTCAAAAGACTAA >OriGene 5' read for NM_001346 unedited
NTGTCAAAATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGGCCAC GGAGATAGACAGCTTTGGAGCTGCTGAACTCCGAGCACAGGGTGAAGACCCCGGCGCTAC CAACCACAGCCTGGCAGCCTGGTCTCCGCGGCACCCACTGGGGCTGCATCCCCCTCCCCC GAGAGGGCTGCGCAGGCGGGAAGACGCCCAGAGGCCAGCTTCGGTCCCCCTTCTGTCTCT CGGTTCCTCTTTCCTCCCAAGTAAGGGAATAAACCGCGAAGAAGGAGCGCCCCGGGCCAC CGCGCAACCAAGTGTTGCCTGGTGAGGAAGAGCCAGGACTTCTGAATTTACCTTGAATAC AGACAGGAGGATGTTGCCTAAGGAATAGCAGAGATCTTGTCTCATCTTCTGAGAGGTGCC TGCTGCTGCTGTATACACTTGAGTGCTCCCAGAAGTCTCCTGAAAGGCTTACATCGCAAA CCTGCAATGAGCCAGGCCCTGGGCTGGGCCTCCACTTCAGCCTAGTGAACAAAACTCCAT CACTGCCCTTTAGCCACTCACATAAAGTTTAAAAATGGGTGAAGAACGGTGGGTCTCCCT CACTCCAGAAGAATTTGACCAACTCCAGAAATATTCAGAATATTCCTCCAAGAAGATAAA AGATGCCTTGACTGAATTTAATGAGGGTGGGAGCCTCANACAATATGACCCACATGAGCC GATTAGCTATGATGTCTTCAAGCTGTTCATGAGGGCGTACCTGGAGGTGGACCTTCCCCA GCCACTGAGCACTCACCTCTTCCTGGCCTTCAGCCAGAAGCCCAGACACGAGACCTCTGA CCACCCGACGGAGGGNAGCCAGCACAGTGAGGCCAACAGCGCAGATACTAATATACAGAA TGCAGATNATGCCACCAAA >OriGene 3' read for NM_001346 unedited
AGGGGNNAGGTNNNNTTTGCCTGCATGCAACTTCCAGGCCAGGAAAGGCACTGGGGAGGG GTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTA GAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACACAATA TTATGACACCAATTTGAAAAATAAATCCCAGTTTCTCCGCTCTCCCTCCCAGGTGTTCAC AGAACTAAGAAAATAAATCCCACATCTAAGGTGTGCTATGCAGGCAGCTCTGGGGGCTCT TGGAGCCGCCTCTAAGCAAACATGTAGACCCCAGGAGAAGGTTCCTGGAAAATAACAAGT GCCCACCGACGGCGCAGAAACCCAAGAATGGAAATACAGCTTTCCACGGCCTTTCTGCCT CTTCAGCAGGTTCACGGAGAAGTTGTCACTCAAAGGACGACCAAGAAACCAACGTGCGCC TGCTGAGCCCTGCCAAGGATTATGCTCTGAGAGCCAAAAGCCGTTTTGTCTCTGTACAGA GGGAACTTTGTGCAAATTCTCTGGAACCAGAGCTTCTTCCCTTGGCCACATTTGATCTCA CCATAGGCAGGAAACCTTGAGCCTTGTTCTCGGGAGCGTCCATGAAGAAACTTTGAGGGG CTGTAGAAAGAAGCGGGATTTCCCTCATCTAAAAAATTCTGCTAAAGCTGTGGGAAATTG TGAGTCTCTANNCANCCCACCCTTTGCCCTAGTCATTCAAGTGTTGTGCACTGCTTTTAA GTTACCT |
| Restriction Sites | Please inquire |
| ACCN | NM_001346 |
| Insert Size | 3900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001346.1, NP_001337.1 |
| RefSeq Size | 3758 bp |
| RefSeq ORF | 2376 bp |
| Locus ID | 1608 |
| UniProt ID | P49619 |
| Domains | DAGKa, DAGKc, EFh, DAG_PE-bind |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| Gene Summary | This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214081 | DGKG (Myc-DDK-tagged)-Human diacylglycerol kinase, gamma 90kDa (DGKG), transcript variant 1 |
CNY 7,890.00 |
|
| RC214081L3 | Lenti-ORF clone of DGKG (Myc-DDK-tagged)-Human diacylglycerol kinase, gamma 90kDa (DGKG), transcript variant 1 |
CNY 9,790.00 |
|
| RC214081L4 | Lenti-ORF clone of DGKG (mGFP-tagged)-Human diacylglycerol kinase, gamma 90kDa (DGKG), transcript variant 1 |
CNY 9,790.00 |
|
| RG214081 | DGKG (tGFP-tagged) - Human diacylglycerol kinase, gamma 90kDa (DGKG), transcript variant 1 |
CNY 7,976.00 |
