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CYP27B1 (NM_000785) Human Untagged Clone
CAT＃: SC123873
CYP27B1 (untagged)-Human cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), nuclear gene encoding mitochondrial protein
CNY 3,792.00
CNY 7,220.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CP2B; CYP1; CYP1alpha; CYP27B; P450c1; PDDR; VDD1; VDDR; VDDRI; VDR |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_000785 edited
GGCGGCCGCGAATTCGGCACGAGGCTGAACCAGACCATGACCCAGACCCTCAAGTACGCC TCCAGAGTGTTCCATCGCGTCCGCTGGGCGCCCGAGTTGGGCGCCTCCCTAGGCTACCGA GAGTACCACTCAGCACGCCGGAGCTTGGCAGACATCCCAGGCCCCTCTACGCCCAGCTTT CTGGCCGAACTTTTCTGCAAGGGGGGGCTGTCGAGGCTACACGAGCTGCAGGTGCAGGGC GCCGCGCACTTCGGGCCGGTGTGGCTAGCCAGCTTTGGGACAGTGCGCACCGTGTACGTG GCTGCCCCTGCACTCGTCGAGGAGCTGCTGCGACAGGAGGGACCCCGGCCCGAGCGCTGC AGCTTCTCGCCCTGGACGGAGCACCGCCGCTGCCGCCAGCGGGCTTGCGGACTGCTCACT GCGGAAGGCGAAGAATGGCAAAGGCTCCGCAGTCTCCTGGCCCCGCTCCTCCTCCGGCCT CAAGCGGCCGCCCGCTACGCCGGAACCCTGAACAACGTAGTCTGCGACCTTGTGCGGCGT CTGAGGCGCCAGCGGGGACGTGGCACGGGGCCGCCCGCCCTGGTTCGGGACGTGGCGGGG GAATTTTACAAGTTCGGACTGGAAGGCATCGCCGCGGTTCTGCTCGGCTCGCGCTTGGGC TGCCTGGAGGCTCAAGTGCCACCCGACACGGAGACCTTCATCCGCGCTGTGGGCTCGGTG TTTGTGTCCACGCTGTTGACCATGGCGATGCCCCACTGGCTGCGCCACCTTGTGCCTGGG CCCTGGGGCCGCCTCTGCCGAGACTGGGACCAGATGTTTGCATTTGCTCAGAGGCACGTG GAGCGGCGAGAGGCAGAGGCAGCCATGAGGAACGGAGGACAGCCCGAGAAGGACCTGGAG TCTGGGGCGCACCTGACCCACTTCCTGTTCCGGGAAGAGTTGCCTGCCCAGTCCATCCTG GGAAATGTGACAGAGTTGCTATTGGCGGGAGTGGACACGGTGTCCAACACGCTCTCTTGG GCTCTGTATGAGCTCTCCCGGCACCCCGAAGTCCAGACAGCACTCCACTCAGAGATCACA GCTGCCCTGAGCCCTGGCTCCAGTGCCTACCCCTCAGCCACTGTTCTGTCCCAGCTGCCC CTGCTGAAGGCGGTGGTCAAGGAAGTGCTAAGACTGTACCCTGTGGTACCTGGAAATTCT CGTGTCCCAGACAAAGACATTCATGTGGGTGACTATATTATCCCCAAAAATACGCTGGTC ACTCTGTGTCACTATGCCACTTCAAGGGACCCTGCCCAGTTCCCAGAGCCAAATTCTTTT CGTCCAGCTCGCTGGCTGGGGGAGGGTCCCACCCCCCACCCATTTGCATCTCTTCCCTTT GGCTTTGGCAAGCGCAGCTGTATGGGGAGACGCCTGGCAGAGCTTGAATTGCAAATGGCT TTGGCCCAGATCCTAACACATTTTGAGGTGCAGCCTGAGCCAGGTGCGGCCCCAGTTAGA CCCAAGACCCGGACTGTCCTGGTACCTGAAAGGAGCATCAACCTACAGTTTTTGGACAGA TAGTCCCATGGAAAGAGACTGTCATCATCACCCTTTCATTCATCATAGGGATAAGATTTT TTGTAGGCACAAGACCAAGGTATACATCTTCCCCTAATGCCTATCTGACCAAACTGGATA GAACCACCATAGTGAAGTGTGAGGCGGCCCTGACCAATGTGTGAAGTATGCACTTGGCCT GACTCAGGAAGCCAGGTGAGAAAACCATGGTCTCTCTGCTTGCTTGGCCCTTCTGATCAT GTATGCATCCCCCAAGGATGAAATCAGATTTTAACTAATAATGCTGGATGGCCTGAGGAA AGATTCAACTGCCTCTCTTTTTGGGCTTTCATAGTGTTCATTGATGCTGCTGGCTAAGCA TTTATCAAAGCATAAGCTCAGTAACTGTGCATCTGGTCTGTACCTGGTTGGTCCTTCGTC TTTGCATGTAAGCTCTTTGAGAGGAAGGGTGAAGCCTTATTTGTTTTTTATGTCCCCTGC CAGGGCCTGTCTCTGACTAGGTGTCACCATACACATTCTTAGATTGAATCTGAACCATGT GGCAGAAGGGATAAGCAGCTTACTTAGTAGGCTCTGTCTACCCCCTTCCTTCTTTGTCTT GCCCCTAGGAAGGTGAATCTGCCCTAGCCTGGTTTACGGTTTCTTATAACTCTCCTTTGC TCTCTGGCCACTATTAAGTGGGTTTGCCCCATCACTTAGTTCTCAGGCAGAGACATCTTT GGGCCTGTCCCTGCCCAGGCCTCTGGCTTTTTATATTGAAAATTTTTAAATATTCACAAA TTTTAGAATAAATCAAATATTCCATTAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGC GGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCC AGTGCCTC >OriGene 5' read for NM_000785 unedited
NGGGTTCCCAATTTGTATACGACNACTATAGGCGGCCGCGNAATTCGCACGAGGCTGAAC CAGACCTGACCCAGACCCTCAGTACGCCTCCAGAGTGTTCCATCGCGTCCGCTGGGCGCC CGAGTTGGGCGCCTCCCTAGGCTACCGAGAGTACCACTCAGCACGCCGGAGCTTGGCAGA CATCCCAGGCCCCTCTACGCCCAGCTTTCTGGCCGAACTTTTCTGCAAGGGGGGGCTGTC GAGGCTACACGAGCTGCAGGTGCAGGGCGCCGCGCACTTCGGGCCGGTGTGGCTAGCCAG CTTTGGGACAGTGCGCACCGTGTACGTGGCTGCCCCTGCACTCGTCGAGGAGCTGCTGCG ACAGGAGGGACCCCGGCCCGAGCGCTGCAGCTTCTCGCCCTGGACGGAGCACCGCCGCTG CCGCCAGCGGGCTTGCGGACTGCTCACTGCGGAAGGCGAAGAATGGCAAAGGCTCCGCAG TCTCCTGGCCCCGCTCCTCCTCCGGCCTCAAGCGGCCGCCCGCTACGCCGGAACCCTGAA CAACGTAGTCTGCGACCTTGTGCGGCGTCTGAGGCGCCAGCGGGGACGTGGCACGGGGCC GCCCGCCCTGGTTCGGGACGTGGCGGGGGAATTTTACAAGTTCGGACTGGAAGGCATCGC CGCGGTTCTGCTCGGCTCGCGCTTGGGCTGCCTGGAGGCTCAAGTGCCACCCGACACGGA GACCTTCATCCGCGCTGTGGGCTCGGTGTTTGTGTCCACGCTGTTGACCATGGCGATGCC CCACTGGCTGCGCCACCTTGTGCCTGGGCCCTGGGGCCGCCTCTGCCGAGACTGGGACCA GATGTTTGCATTTGCTCAGAGCACNNTGGAGCGCNAGAGGCANAAGCAGCCATNGAGAAC GGNAGACAGCCCGAGAAG >OriGene 3' read for NM_000785 unedited
GGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATGGAATATTTGAT TTATTCTAAAATTTGTGAATATTTAAAAATTTTCAATATAAAAAGCCAGAGGCCTGGGCA GGGACAGGCCCAAAGATGTCTCTGCCTGAGAACTAAGTGATGGGGCAAACCCACTTAATA GTGGCCAGAGAGCAAAGGAGAGTTATAAGAAACCGTAAACCAGGCTAGGGCAGATTCACC TTCCTAGGGGCAAGACAAAGAAGGAAGGGGGTAGACAGAGCCTACTAAGTAAGCTGCTTA TCCCTTCTGCCACATGGTTCAGATTCAATCTAAGAATGTGTATGGTGACACCTAGTCAGA GACAGGCCCTGGCAGGGGACATAAAAAACAAATAAGGCTTCACCCTTCCTCTCAAAGAGC TTACATGCAAAGACGAAGGACCAACCAGGTACAGACCAGATGCACAGTTACTGAGCTTAT GCTTTGATAAATGCTTAGCCAGCAGCATCAATGAACACTATGAAAGCCCAAAAAGAGAGG CAGTTGAATCTTTCCTCAGGCCATCCAGCATTATTAGTTAAAATCTGATTTCATCCTTGG NGGATGCATACATGATCAGAAGGGCCAAGCAAGCAGAGAGACCATGGTTTTCTCACCTGG CTTCCTGAGTCAGGCCAAGTGCAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000785 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000785.3, NP_000776.1 |
| RefSeq Size | 2503 bp |
| RefSeq ORF | 1527 bp |
| Locus ID | 1594 |
| UniProt ID | O15528 |
| Protein Families | Druggable Genome, P450 |
| Protein Pathways | Metabolic pathways, Steroid biosynthesis |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
25(OH)D Is Effective to Repress Human Cholangiocarcinoma Cell Growth through the Conversion of 25(OH)D to 1a,25(OH)2D3
,Chiang, KC;Yeh, CN;Huang, CC;Yeh, TS;S Pang, JH;Hsu, JT;Chen, LW;Kuo, SF;Kittaka, A;Chen, TC;Juang, HH;,
Int J Mol Sci
,PubMed ID 27529229
[CYP27B1]
|
|
Hepatocellular carcinoma cells express 25(OH)D-1a-hydroxylase and are able to convert 25(OH)D to 1a,25(OH)2D, leading to the 25(OH)D-induced growth inhibition
,Chiang, KC;Yen, CL;Yeh, CN;Hsu, JT;Chen, LW;Kuo, SF;Wang, SY;Sun, CC;Kittaka, A;Chen, TC;Yeh, TS;Hsu, SY;Juang, HH;,
J. Steroid Biochem. Mol. Biol.
,PubMed ID 26170242
[CYP27B1]
|
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222840 | CYP27B1 (Myc-DDK-tagged)-Human cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), nuclear gene encoding mitochondrial protein |
CNY 3,784.00 |
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| RC222840L1 | Lenti ORF clone of Human cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,184.00 |
|
| RC222840L2 | Lenti ORF clone of Human cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,460.00 |
|
| RC222840L3 | Lenti ORF clone of Human cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC222840L4 | Lenti ORF clone of Human cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,460.00 |
|
| RG222840 | CYP27B1 (tGFP-tagged) - Human cytochrome P450, family 27, subfamily B, polypeptide 1 (CYP27B1), nuclear gene encoding mitochondrial protein |
CNY 5,384.00 |
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