POFUT2 (BC000626) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C21orf80; FUT13
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC000626 edited GGCACGAGGGAAGCGTCATTTTAAATGTCTGCAGAGCGACCAGGGGCCTCATGAATCTCT CCGTTGCCCTCCGCGCAGCAGGAGGCTGCCTGTGTGTTTCCTCCTGGGATGCGTGCAAGG CAGACCTGGTGCTGCAAAGGAAAGGGCCTGAGGCCTCAGGGAGCCCCGTGGAGGGATGAC AGTTCAGGCCCTACTGCTGGCACGTCAGAGCACTGGGAAGTTTTTCAGTGACGTCTCTGG GGCACTCAGTGGATTGTCTGTAGGAAACTTGCAGCTCTGCTCCTCACACCAGGCCCGGCT GGCCACCCACCCTCGCCCCCACTGGCCACCCCTCCCTCGCCCCGACTGCCCCGCCCCACC CTCACCCCGACTGCCCCGCCCTCGCCCGGCTGGCCGTCCCTGCCCTCGCCCCGGCTGGCA GGTGCACATGGGGCCTCCAGGTCTGCCATTCGCTATTGAGAACTAGAAATGAGGAAGGAC AGTTACGCTAACTCCAAAAGGCTGTCTAGGATGAGCTGCTTTATCAGGGAGCTCCTTGTA CCCATTTTACAGAAATCATTTTTAGGTCTTTGTGCCACCACCACGAGGGGCATCTGCAAA GAGGGCAACGCTAGACACAGAATCCGTGGAAGGTGCAGCAGTGCCTCAGGGGTCCTCAGG GTCAGGGAGCCCCCCTCACCCTCTTGGCCCGTTACCCTTTGTGACTTTCCACCATGGTGT CGTGTGACCCTCAGTCAGGTTGGTGGGGGCTGAGTCCTCACTGAGCAGCCACTTTCCACA TCTGCTAGAGGAACAGTGACATGGACACCTGTGACAGAGAGAGGACAGTTAGGAGGGACA GACAGCTCTTCCTTTCGGAGCCTGGCTAGTCTAGGACATCACCTTGCTGTGTCTTCTCAA GCTTTTAAAATTGACCCTGAACGTGTGACAGGGTCCTATGGTGTTACTCAAAGCTGTGCA GGGTAAATGATGACATATTTATTCTTTTTCCATTTGTTCTAGAAACAGTGCCTTTTTCAT CAGTTGCATTTTCCAGGCTGAGAGCTGTATAAAACATTTTGGACTGTGACCATGTACCTT CCTTTTTAAGAAAAATAAACTGCTTTATGGAAGTTGGTAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC000626 unedited TGTCAGAATTGTACTTCACTCATATAGGCGGCCAGCGAATTCGCACGAGGGNAAGCGTCA TTTTAAATGTCTGCAGAGCGACCAGGGGCCTCATGAATCTCTCCGTTGCCCTCCGCGCAG CAGGAGGCTGCCTGTGTGTTTCCTCCTGGGATGCGTGCAAGGCAGACCTGGTGCTGCAAA GGAAAGGGCCTGAGGCCTCAGGGAGCCCCGTGGAGGGATGACAGTTCAGGCCCTACTGCT GGCACGTCAGAGCACTGGGAAGTTTTTCAGTGACGTCTCTGGGGCACTCAGTGGATTGTC TGTAGGAAACTTGCAGCTCTGCTCCTCACACCAGGCCCGGCTGGCCACCCACCCTCGCCC CCACTGGCCACCCCTCCCTCGCCCCGACTGCCCCGCCCCACCCTCACCCCGACTGCCCCG CCCTCGCCCGGCTGGCCGTCCCTGCCCTCGCCCCGGCTGGCAGGTGCACATGGGGCCTCC AGGTCTGCCATTCGCTATTGAGAACTAGAAATGAGGAAGGACAGTTACGCTAACTCCAAA AGGCTGTCTAGGATGAGCTGCTTTATCAGGGAGCTCCTTGTACCCATTTTACAGAAATCA TTTTTAGGTCTTTGTGCCACCACCACGAGGGGCATCTGCAAAGAGGGCAACGCTAGACAC AGAATCCGTGGAAGGTGCAGCAGTGCCTCAGGGGTCCTCAGGGTCAGGGAGCCCCCCTCA CCCTCTTGGCCCGTTACCCTTTGTGACTTTCCACCATGGTGTCGTGTGACCCTCAGTCAG GTTGGTGGGGGCTGAGTCCTCACTGAGCAGCCACTTTCCACATCTGCTAGAGGAACAGTG ACATGGACACCTGTGACAGAGAGAGGACAGTTAGGAGGGACAGACAGCTCTTCCTTTNCG AGCCTGNCTAGTNCTAGACATCACTT
Restriction Sites	Please inquire
ACCN	BC000626
Insert Size	1139 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC000626.1, AAH00626.1
RefSeq Size	1139 bp
Locus ID	23275
Gene Summary	Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008]

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