AP4E1 (BC000772) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC000772 edited GGCACGAGGCCATGTTAGTCAGGCTGGTCTTGAACTCCTGGTATCAAGTGATCTGCCCGC CTGAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGCGCCCGGCCCCTGTCCTC CCCCTTTTACAGATGAGGCAGCTGAGGCCCAGAGTCACAGGGCTGGTGAGGAACACAGCC TGGATCAGAGCCCAGGAAGCCTGGGTCAAGGCTCACGTCTCTTGAGTGAGGGGCTCTGCC CTCCTCCATCCTGTTCTTTGTAGTGTCATCTTTCATGACCTACATGGAGACATTCAAATC CGGGGATGACACAGAGCTTGGAGGGAGAGACGTGTCAGGAGACAGAAACAGTGTCCCTGG AGATATCAGCAGGCAAGTGTGCTGAGCTCACACTGAGAGCCAGAGGGGATTCTGAAAACT AGACTGATGATTAAAACTGCTTAAAAACTCAGCAGCCTCCCCATGCTCCCGGGATTAATG ATGCAGGCTCACATTGACTACACACGTTTATACCAGGCGCCATGTTCTTTGCCGAAACTC CCGCTCAGAGCCTGGATGATCTGCCCCTCACCTCTGCCCTCGTTCCCATCCACTCTCCCC TCCTTGCTCTGTTCCAGCCACACTGGCCTCTTGCTGTTCCTCACACTCTCCACGTCCAGG CACACCCCAGGGCCTTTGCACTGGCTCTCACTGCTGCCTCCAGTGAGCTCCCTCCAGATC TTCTTGGGTGCAGCTCATGAGGACTCGCTCTAATGCCACCCCCTCAAACAGGTCCTCCTG GCCTTCAGTAGCCCAGGCTGTCATTCTGTGTCACTGTACTCTGTTCCACATCCCGTGTCC CCCATGGGAAACAGGTGCCACCGTCTTAGTCACAAGGCACTTGTAACGTGCCTGGCACAC AGGGCATGCTCCATCCTGAACATAAGACGTGTGGATCTTGTTTGATTCTCACGTGACCCC AATAAGTAGATACTATAAGGCCCCCACTTTTCAGTGTGGCCCAAGTTGGGCCCTGCTGAA CCCTGTTACCCTCCTCAACAATCACCCGTACACACTGCTTCCTCTGCCTGGAACCCACCC CCCAACCCGCACCCCCACTCTAGGCCCACTCCTGCCCACCCATGTTAATTTGCTGGGGCA GCCGCAACAAACTACCACACACTGAGTGGCTTAAACTGCAGAAATGTATCATCTCACAGT TGCGGAGGCCAGAAGTCCAAGTTCCAGGCGTCAACAGGGTGGTTCCCTCCAAGGGCGGTG AGGGAGAACCTGTTCCTGGCCTCCCTCTTAGCAGCCGGTGGGTGTCAGCACGTAGATTCA TCACCCCCGTCTCTACCTCCTCTTCGCATGATGTTCTCCCTGCATCTGTGCCCACATTTC CTCTTTCTGCAAGGACGCCAATCTAACGGGATTAGGGGTCTACCCTAATGACCTCATTTT CACTTGATTGCCTCTGTAAAGACTCTGTCTCCAAATGAGGTCACATTTGGAGATGCTGGG GCTTAGGACTTCAATGTAGCTTTTTGTGGGGCCCCAGTTCAACCCATAACACCACTCCTT GGGTATCAACACAGACACGCCTTTCTTGCAGAAGTTGTCTGAACCCCCACACCAGGCTCA GCCCACCCCCAGCACCTGTCCCAGGGCACAGCGACCGCCACTCCCTTGGCAACTTGCTTC CTCTCTGTCACCCCCATGGGGCCCAGAACAGAGACATCCTGAACCTCACCCAGACCACAC ACTTAGTAGGTGCTCAGAAGATGTACCTGGAGAGACAACAACCCTATCCAACAACAGCTT TCCCAGTGATGAAATCCAGAGATGGCTTGTATATGTGTGTGTTGGTGTAAACATATGTAA ACTAAGCATATAAAATATCAGCAGTAGACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC000772 unedited NGGGATAGCGTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCC ATGTTAGTCAGGCTGGTCTTGAACTCCTGGTATCAAGTGATCTGCCCGCCTGAGCCTCCC AAATGCTGGGATTACAGGCGTGAGCCACTGCGCCCGGCCCCTGTCCTCCCCCTTTTACAG ATGAGGCAGCTGAGGCCCAGAGTCACAGGGCTGGTGAGGAACACAGCCTGGATCAGAGCC CAGGAAGCCTGGGTCAAGGCTCACGTCTCTTGAGTGAGGGGCTCTGCCCTCCTCCATCCT GTTCTTTGTAGTGTCATCTTTCATGACCTACATGGAGACATTCAAATCCGGGGATGACAC AGAGCTTGGAGGGAGAGACGTGTCAGGAGACAGAAACAGTGTCCCTGGAGATATCAGCAG GCAAGTGTGCTGAGCTCACACTGAGAGCCAGAGGGGATTCTGAAAACTAGACTGATGATT AAAACTGCTTAAAAACTCAGCAGCCTCCCCATGCTCCCGGGATTAATGATGCAGGCTCAC ATTGACTACACACGTTTATACCAGGCGCCATGTTCTTTGCCGAAACTCCCGCTCAGAGCC TGGATGATCTGCCCCTCACCTCTGCCCTCGTTCCCATCCACTCTCCCCTCCTTGCTCTGT TCCAGCCACACTGGCCTCTTGCTGTTCCTCACACTCTCCACGTCCAGGCACACCCCAGGG CCTTTGCACTGGCTCTCACTGCTGCCTCCAGTGAGCTCCCTCCAGATCTTCTTGGGTGCA GCTCATGAGGACTCGCTCTAATGCCACCCCCTCAAACAGGTCCTCCTGGCCTTCAGTAGC CCAGCTGTCATTCTGTGTCACTGTACTCTGTTCCACATCCCGTGTCCC
Restriction Sites	Please inquire
ACCN	BC000772
Insert Size	1945 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC000772.1, AAH00772.1
RefSeq Size	1945 bp
Locus ID	23431
Protein Pathways	Lysosome
Gene Summary	This gene encodes a member of the adaptor complexes large subunit protein family. These proteins are components of the heterotetrameric adaptor protein complexes, which play important roles in the secretory and endocytic pathways by mediating vesicle formation and sorting of integral membrane proteins. The encoded protein is a large subunit of adaptor protein complex-4, which is associated with both clathrin- and nonclathrin-coated vesicles. Disruption of this gene may be associated with cerebral palsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

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