UBE2B (BC001694) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	E2-17kDa; HHR6B; HR6B; RAD6B; UBC2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC001694 edited GGCACGAGGGGAGTCGGGAGAGGGGTTCGCCCCAACACGGCTCCTCGGAGAAGACGGCGG GGCCGTGAAACAAAGCTGACTGGTGCTGCCGGAGAGGAACAAGCTCCCGCAGGAGCTCAC TTTGAGGGGACAAGAAGGGGGCGCCGCAGGGTGCAGGGGCGGGCGGATAATGTCTGATCG CCTGGGCGAGACGCCGGGCAGCTGGCCGAAATGGGCAGCAGGGAACCCTAGACCAGTCGA TCGACACCGTTTTACTTTTTGTGGGGAGGGGAAGCAGTATCAAAATTTCTTCGGGCGCCA GAACGAGCGGCAAAGCTTATGGGAGTAGGGCCGAGGCTAGGACCCGGCTTCCGCCAGTCC CGCTGGGCCCACAGCCGGCCCTCTGAGGGTCACAAAGGGGAAGGTGTCTGGGATCCACGC CCTGCAGCCCCGCAGCTTTGAGGGGCCGTCATCTAGGCGAAGGTGAAGGCCCTTACCGCT TGAAATCCCGCATGAGCCTCCTCCGGGCCGGGGTCGACATGCTCCGCAGCTGCCCCGCGG TCAGTCTGAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC001694 unedited GCACGAGGGGAGTCGGGAGAGGGGTTCGCCCCAACACGGCTCCTCGGAGAAGACGGCGGG GCCGTGAAACAAAGCTGACTGGTGCTGCCGGAGAGGAACAAGCTCCCGCAGGAGCTCACT TTGAGGGGACAAGAAGGGGGCGCCGCAGGGTGCAGGGGCGGGCGGATAATGTCTGATCGC CTGGGCGAGACGCCGGGCAGCTGGCCGAAATGGGCAGCAGGGAACCCTAGACCAGTCGAT CGACACCGTTTTACTTTTTGTGGGGAGGGGAAGCAGTATCAAAATTTCTTCGGGCGCCAG AACGAGCGGCAAAGCTTATGGGAGTAGGGCCGAGGCTAGGACCCGGCTTCCGCCAGTCCC GCTGGGCCCACAGCCGGCCCTCTGAGGGTCACAAAGGGGAAGGTGTCTGGGATCCACGCC CTGCAGCCCCGCAGCTTTGAGGGGCCGTCATCTAGGCGAAGGTGAAGGCCCTTACCGCTT GAAATCCCGCATGAGCCTCCTCCGGGCCGGGGTCGACATGCTCCGCAGCTGCCCCGCGGT CAGTCTGAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAGTT
Restriction Sites	Please inquire
ACCN	BC001694
Insert Size	574 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC001694.1, AAH01694.1
RefSeq Size	574 bp
Locus ID	7320
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
Gene Summary	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]

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