MCHR (MCHR1) (BC001736) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	G-protein coupled receptor 24; GPR24; G protein-coupled receptor 24; MCH1R; melanin-concentrating hormone receptor 1; MGC32129; SLC1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC001736 edited GCAAGGCAAGCAGCAGCCCCTGTAAGCACACGAGACAATCCCAAGTGTCAGTGGGAAGGA GATCCCTTTCCTGATGGGGCTGCCTGTGTCCAGTCCCTCCCAGCTTCCCCAGGGCCCTGG GGCTCTGCAGGCATTCAGAAGTGGAAGCCAGCCACAGCCTGGGACTGAAGAGGTTAATGT GCATCTGCCTCCGAATGTTAATGTGTCTAGGTGATGTCAGTGGGAGCCATGAAGAAGGGA GTGGGGAGGGCAGTTGGGCTTGGAGGCGGCAGCGGCTGCCAGGCTACGGAGGAAGACCCC CTTCCCGACTGCGGGGCTTGCGCTCCGGGACAAGGTGGCAGGCGCTGGAGGCTGCCGCAG CCTGCGTGGGTGGAGGGGAGCTCAGCTCGGTTGTGGGAGCAGGCGACCGGCACTGGCTGG ATGGACCTGGAAGCCTCGCTGCTGCCCACTGGTCCCAATGCCAGCAACACCTCTGATGGC CCCGATAACCTCACTTCGGCAGGATCACCTCCTCGCACGGGGAGCATCTCCTACATCAAC ATCATCATGCCTTCGGTGTTCGGCACCATCTGCCTCCTGGGCATCATCGGGAACTCCACG GTCATCTTCGCGGTCGTGAAGAAGTCCAAGCTGCACTGGTGCAACAACGTCCCCGACATC TTCATCATCAACCTCTCGGTAGTAGATCTCCTCTTTCTCCTGGGCATGCCCTTCATGATC CACCAGCTCATGGGCAATGGGGTGTGGCACTTTGGGGAGACCATGTGCACCCTCATCACG GCCATGGATGCCAATAGTCAGTTCACCAGCACCTACATCCTGACCGCCATGGCCATTGAC CGCTACCTGGCCACTGTCCACCCCATCTCTTCCACGAAGTTCCGGAAGCCCTCTGTGGCC ACCCTGGTGATCTGCCTCCTGTGGGCCCTCTCCTTCATCAGCATCACCCCTGTGTGGCTG TATGCCAGACTCATCCCCTTCCCAGGAGGTGCAGTGGGCTGCGGCATACGCCTGCCCAAC CCAGACACTGACCTCTACTGGTTCACCCTGTACCAGTTTTTCCTGGCCTTTGCCCTGCCT TTTGTGGTCATCACAGCCGCATACGTGAGGATCCTGCAGCGCATGACGTCCTCAGTGGCC CCCGCCTCCCAGCGCAGCATCCGGCTGCGGACAAAGAGGGTGACCCGCACAGCCATCGCC ATCTGTCTGGTCTTCTTTGTGTGCTGGGCACCCTACTATGTGCTACAGCTGACCCAGTTG TCCATCAGCCGCCCGACCCTCACCTTTGTCTACTTATACAATGCGGCCATCAGCTTGGGC TATGCCAACAGCTGCCTCAACCCCTTTGTGTACATCGTGCTCTGTGAGACGTTCCGCAAA CGCTTGGTCCTGTCGGTGAAGCCTGCAGCCCAGGGGCAGCTTCGCGCTGTCAGCAACGCT CAGACGGCTGACGAGGAGAGGACAGAAAGCAAAGGCACCTGATACTTCCCCTGCCACCCT GCACACCTCCAAGTCAGGGCACCACAACACGCCACCGGGAGAGATGCTGAGAAAAACCCA AGACCGCTCGGGAAATGCAGGAAGGCCGGGTTGTGAGGGGTTGTTGCAATGAAATAAATA CATTCCATGGGGCTCACACGTTGCTGGGGAGGCCTGGAGTCAGGTTTGGGGTTTTCAGAT ATCAGAAATCCCCTTGGGGGAGCAGGATGAGACCTTTGGATAGAACAGAAGCTGAGCAAG AGAACATGTTGGTTTGGATAACCGGTTGCACTATATCTGTGAGCTCTCAAATGTCTTCTT CCCAAGGCAAGAGGTGGAAGGGTACTGACTGGGTTTGTTTAAAGTCAGGCAGGGCTGGAG TGAGCAGCCAGGGCCATGTTGCACAAGGCCTGAGAGACGGGAAAGGGCCCGATCGCTCTT TCCCGCCTCTCACTGGTGCGATGGAAGGTGGCCTTTCTCCCAAGCTGGTGGATAATGAAA AATAAAGCACCTTCTCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC001736 unedited TGTCAGAATTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCAAGGCAA GCAGCAGCCCCTGTAAGCACACGAGACAATCCCAAGTGTCAGTGGGAAGGAGATCCCTTT CCTGATGGGGCTGCCTGTGTCCAGTCCCTCCCAGCTTCCCCAGGGCCCTGGGGCTCTGCA GGCATTCAGAAGTGGAAGCCAGCCACAGCCTGGGACTGAAGAGGTTAATGTGCATCTGCC TCCGAATGTTAATGTGTCTAGGTGATGTCAGTGGGAGCCATGAAGAAGGGAGTGGGGAGG GCAGTTGGGCTTGGAGGCGGCAGCGGCTGCCAGGCTACGGAGGAAGACCCCCTTCCCGAC TGCGGGGCTTGCGCTCCGGGACAAGGTGGCAGGCGCTGGAGGCTGCCGCAGCCTGCGTGG GTGGAGGGGAGCTCAGCTCGGTTGTGGGAGCAGGCGACCGGCACTGGCTGGATGGACCTG GAAGCCTCGCTGCTGCCCACTGGTCCCAATGCCAGCAACACCTCTGATGGCCCCGATAAC CTCACTTCGGCAGGATCACCTCCTCGCACGGGGAGCATCTCCTACATCAACATCATCATG CCTTCGGTGTTCGGCACCATCTGCCTCCTGGGCATCATCGGGAACTCCACGGTCATCTTC GCNGTCGTGAAGAAGTCCAAGCTGCACTGGTGCAAACACGTCCCCGACATCTTCATCATC AACCTCTCGGTAGTAGATCTCCTCTTTCTCCTGNGCATGCCCTTCATGATCCACCAGCTC ATGGGCAATNGGGGTGTGGCACTTTGGGGAGACCATGTGCACCCTCATCACGGCCATGGA TGCCAATAGTCAGTTCACCAGCACCTACATCCTGACCGCCATGGCCATTGACCGCTACCT GGCCACTGTCCACCCATCTCTTNCACGAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC001736 unedited CCGGGGGACATTGGNNGATGGCACTTNCCAGGNCCAGNANAGCACTGGGGNAGGGTCACA GGGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCG AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGGAAAAGGTGCTT TATTTTTCATTATCCACCAGCTTGGGAGAAAGGCCACCTTCCATCGCACCAGTGAGAGGC GGGAAAAAGCGATCGGGCCCTTTCCCGTCTCTCAGGCCTTGTGCAACATGGCCCTGGCTG CTCACTCCAGCCCTGCCTGACTTTAAACAAACCCAGTCAGTACCCTTCCACCTCTTGCCT TGGGAAAAAAACATTTGAAAGCTCACAGATATAGTGCAACCGGTTATCCAAACCAACATG TTCTCTTGCTCAACTTCTGTTCTATCCAAAGGTCTCATCCTGCTCCCCCAAGGGGATTTC TGATATCTGAAAACCCCAAACCTGACTCCAGGCCTCCCCAGCAACGTGTGAGCCCCATGG AATGTATTTATTTTATTGGAACAACCCCTCACAACCCGGCCTTCCTGCATTTCCCGAGCG GTCTTGGGTTTTTCTCAACATCTCTCCCGGTGGCGTGTTGTGGTGCCCTGACTTGGAGGT GTGCACGGTGGCAGGGGAAGTATCAAGTGCCTTTGCTTTCTGTCCTCTCCTCGTCCACCC TCTGAACGTTGCTGACAGCGCGAAACTGCCCCTGGGCTGCAGGCTTAACCGACAGGGACC AAACGTTTGCGGAACGTCTTTAAAGAACCGGATGTCAACCAAGGGGTTTGAGGCACCTGT TTGCTTAACCCCAAGCTTGTGGGCCGCATTGTTTAAATAGACAAAG
Restriction Sites	Please inquire
ACCN	BC001736
Insert Size	2150 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC001736.1, AAH01736.1
RefSeq Size	2042 bp
RefSeq ORF	1266 bp
Locus ID	2847
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction
Gene Summary	The protein encoded by this gene, a member of the G protein-coupled receptor family 1, is an integral plasma membrane protein which binds melanin-concentrating hormone. The encoded protein can inhibit cAMP accumulation and stimulate intracellular calcium flux, and is probably involved in the neuronal regulation of food consumption. Although structurally similar to somatostatin receptors, this protein does not seem to bind somatostatin. [provided by RefSeq, Jul 2008]

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