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Glucokinase (GCK) (NM_000162) Human Untagged Clone
CAT＃: SC123784
GCK (untagged)-Human glucokinase (hexokinase 4) (GCK), transcript variant 1
CNY 3,656.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FGQTL3; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2; PNDM1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_000162 edited
GAGCAGGAAATGCCGAGCGGCGCCTGAGCCCCAGGGAAGCAGGCTAGGATGTGAGAGACA CAGTCACCTGCAGCCTAATTACTCAAAAGCTGTCCCCAGGTCACAGAAGGGAGAGGACAT TTCCCACTGAATCTGTCTGAAGGACACTAAGCCCCACAGCTCAACACAACCAGGAGAGAA AGCGCTGAGGACGCCACCCAAGCGCCCAGCAATGGCCCTGCCTGGAGAACATCCAGGCTC AGTGAGGAAGGGTCCAGAAGGGAATGCTTGCCGACTCGTTGGAGAACAATGAAAAGGAGG AAACTGTGACTGAACCTCAAACCCCAAACCAGCCCGAGGAGAACCACATTCTCCCAGGGA CCCAGGGCGGGCCGTGACCCCTGCGGCGGAGAAGCCTTGGATATTTCCACTTCAGAAGCC TACTGGGGAAGGCTGAGGGGTCCCAGCTCCCCACGCTGGCTGCTGTGCAGATGCTGGACG ACAGAGCCAGGATGGAGGCCGCCAAGAAGGAGAAGGTAGAGCAGATCCTGGCAGAGTTCC AGCTGCAGGAGGAGGACCTGAAGAAGGTGATGAGACGGATGCAGAAGGAGATGGACCGCG GCCTGAGGCTGGAGACCCATGAAGAGGCCAGTGTGAAGATGCTGCCCACCTACGTGCGCT CCACCCCAGAAGGCTCAGAAGTCGGGGACTTCCTCTCCCTGGACCTGGGTGGCACTAACT TCAGGGTGATGCTGGTGAAGGTGGGAGAAGGTGAGGAGGGGCAGTGGAGCGTGAAGACCA AACACCAGATGTACTCCATCCCCGAGGACGCCATGACCGGCACTGCTGAGATGCTCTTCG ACTACATCTCTGAGTGCATCTCCGACTTCCTGGACAAGCATCAGATGAAACACAAGAAGC TGCCCCTGGGCTTCACCTTCTCCTTTCCTGTGAGGCACGAAGACATCGATAAGGGCATCC TTCTCAACTGGACCAAGGGCTTCAAGGCCTCAGGAGCAGAAGGGAACAATGTCGTGGGGC TTCTGCGAGACGCTATCAAACGGAGAGGGGACTTTGAAATGGATGTGGTGGCAATGGTGA ATGACACGGTGGCCACGATGATCTCCTGCTACTACGAAGACCATCAGTGCGAGGTCGGCA TGATCGTGGGCACGGGCTGCAATGCCTGCTACATGGAGGAGATGCAGAATGTGGAGCTGG TGGAGGGGGACGAGGGCCGCATGTGCGTCAATACCGAGTGGGGCGCCTTCGGGGACTCCG GCGAGCTGGACGAGTTCCTGCTGGAGTATGACCGCCTGGTGGACGAGAGCTCTGCAAACC CCGGTCAGCAGCTGTATGAGAAGCTCATAGGTGGCAAGTACATGGGCGAGCTGGTGCGGC TTGTGCTGCTCAGGCTCGTGGACGAAAACCTGCTCTTCCACGGGGAGGCCTCCGAGCAGC TGCGCACACGCGGAGCCTTCGAGACGCGCTTCGTGTCGCAGGTGGAGAGCGACACGGGCG ACCGCAAGCAGATCTACAACATCCTGAGCACGCTGGGGCTGCGACCCTCGACCACCGACT GCGACATCGTGCGCCGCGCCTGCGAGAGCGTGTCTACGCGCGCTGCGCACATGTGCTCGG CGGGGCTGGCGGGCGTCATCAACCGCATGCGCGAGAGCCGCAGCGAGGACGTAATGCGCA TCACTGTGGGCGTGGATGGCTCCGTGTACAAGCTGCACCCCAGCTTCAAGGAGCGGTTCC ATGCCAGCGTGCGCAGGCTGACGCCCAGCTGCGAGATCACCTTCATCGAGTCGGAGGAGG GCAGTGGCCGGGGCGCGGCCCTGGTCTCGGCGGTGGCCTGTAAGAAGGCCTGTATGCTGG GCCAGTGAGAGCAGTGGCCGCAAGCGCAGGGAGGATGCCACAGCCCCACAGCACCCAGGC TCCATGGGGAAGTGCTCCCCACACGTGCTCGCAGCCTGGCGGGGCAGGAGGCCTGGCCTT GTCAGGACCCAGGCCGCCTGCCATACCGCTGGGGAACAGAGCGGGCCTCTTCCCTCAGTT TTTCGGTGGGACAGCCCCAGGGCCCTAACGGGGGTGCGGCAGGAGCAGGAACAGAGACTC TGGAAGCCCCCCACCTTTCTCGCTGGAATCAATTTCCCAGAAGGGAGTTGCTCACTCAGG ACTTTGATGCATTTCCACACTGTCAGAGCTGTTGGCCTCGCCTGGGCCCAGGCTCTGGGA AGGGGTGCCCTCTGGATCCTGCTGTGGCCTCACTTCCCTGGGAACTCATCCTGTGTGGGG AGGCAGCTCCAACAGCTTGACCAGACCTAGACCTGGGCCAAAAGGGCAGCCAGGGGCTGC TCATCACCCAGTCCTGGCCATTTTCTTGCCTGAGGCTCAAGAGGCCCAGGGAGCAATGGG AGGGGGCTCCATGGAGGAGGTGTCCCAAGCTTTGAATACCCCCAGAGACCTTTTCTCTCC CATACCATCACTGAGTGGCTTGTGATTCTGGGATGGACCCTCGCAGCAGGTGCAAGAGAC AGAGCCCCCAAGCCTCTGCCCCAAGGGGCCCACAAAGGGGAGAAGGGCCAGCCCTACATC TTCAGCTCCCATAGCGCTGGCTCAGGAAGAAACCCCAAGCAGCATTCAGCACACCCCAAG GGACAACCCCATCATATGACATGCCACCCTCTCCATGCCCAACCTAAGATTGTGTGGGTT TTTTAATTAAAAATGTTAAAAGTTTTAAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_000162 unedited
GGCAGGACTTCNGTTAANNTNTAAAACACGNATTTACTNAAAGGGNCGGNNCGCGNAATT CGGCACGAGGGAAGCAGGTAAATGCCGAGCGGCGCCTNGAGCCCCAGGAAAGCAGGCTAG GATGTGAGAGACACAGTCACCTGCAGCCTAATTACTCAAAAGCTGTCCCCAGGTCACAGA AGGGAGAGGACATTTCCCACTGAATCTGTCTGAAGGACACTAAGCCCCACAGCTCAACAC AACCAGGAGAGAAAGCGCTGAGGACGCCACCCAAGCGCCCAGCAATGGCCCTGCCTGGAG AACATCCAGGCTCAGTGAGGAAGGGTCCAGAAGGGAATGCTTGCCGACTCGTTGGAGAAC AATGAAAAGGAGGAAACTGTGACTGAACCTCAAACCCCAAACCAGCCCGAGGAGAACCAC ATTCTCCCAGGGACCCAGGGCGGGCCGTGACCCCTGCGGCGGAGAAGCCTTGGATATTTC CACTTCAGAAGCCTACTGGGGAAGGCTGAGGGGTCCCAGCTCCCCACGCTGGCTGCTGTG CAGATGCTGGACGACAGAGCCAGGATGGAGGCCGCCAAGAAGGAGAAGGTAGAGCAGATC CTGGCAGAGTTCCAGCTGCAGGAGGAGGACCTGAAGAAGGTGATGAGACGGATGCAGAAG GAGATGGACCGCGGCCTGAGGCTGGAGACCCATGAAGAGGCCAGTGTGAAGATGCTGCCC ACCTACGTGCGCTCCACCCCAGAAGGCTCAGAAGTCGGGGACTTCCTCTCCCTGGACCTG TGTGGCACTAACTTCANGGTGATGCTGGTGAAGGTGGGAAAAGTGAGGAGGGGCAGTGGA GCGTGAAGACAACACCAGAGTACTCCATCCCCGAGGACGCCTGACCCGCACTGCTGAGAG CTCTTCACTCA >OriGene 3' read for NM_000162 unedited
GGGGCCATTGGGCGATGGTCAACTTGCCAGGNCCAGGATAGCACTGGGGNAGGGTCACAG GGCTGCCACCCGGGCTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGA GTTTTTTTTTTTNTTTTTTTTTTTAAAACTTTTAACATTTTTAATTAAAAAACCCACACA ATCTTATGTTGGGCATGGAGAGGGTGGCATGTCATATGATGGGGTTGTCCCTTGGGGTGT GCTGAATGCTGCTTGGGGTTTCTTCCTGAGCCAGCGCTATGGGAGCTGAATATGTAGGGC TGGCCCTTCTCCCCTTTGTGGGCCCCTTGGGGCAGAAGCTTGGGGGCTCTGTCTCTTGCA CCTGCTGCGAGGGTCCATCCCAGAATCACAAGCCACTCAGTGATGGTATGGGAGAGAAAA GGTCTCTGGGGGTATTCAAAGCTTGGGACACCTCCTCCATGGAGCCCCCTCCCATTGCTC CCTGGGCCTCTTGAGCCTCAGGCAAGAAAATGGCCAGGACTGGGTGATGAGCAGCCCCTG GCTGCCCTTTTGGCCCAAGTCTAAGTCTGGTCAAGCTGTTGGAGCTGCCTCCCCACACAG GATGAGTTCCCAGGGAAGTGAGGCCACAGCAGGATCCAGAGGGCACCCCTTCCCAGAGCC TGGGCCCAGGCGAGGCCAACAGCTCTGACAGTGTGGAAATGCATCAAAGTCCTGAGTGAG CAACTCCCTTCTGGGAAATTGATTCCAGCGAGAAAGGTGGGGGGGCTTCCAGAGTCTCTG TTCCTGCTCCTGCCGCACCCCCGTTAGGGCCCTGGGGCTGTCCCACCGAAAACTGAGGGA AATAGCCCGCTCTTTACCACGTTTTGGGGAAAGCCGCCTTGGTTTCCTGAAAGGCCAGGT CCTTCTGCCCCGACAT |
| Restriction Sites | Please inquire |
| ACCN | NM_000162 |
| Insert Size | 2740 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000162.2, NP_000153.1 |
| RefSeq Size | 2759 bp |
| RefSeq ORF | 1398 bp |
| Locus ID | 2645 |
| UniProt ID | P35557 |
| Protein Families | Druggable Genome |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Maturity onset diabetes of the young, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus |
| Gene Summary | This gene encodes a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (1) encodes the isoform expressed specifically in pancreatic islet beta cells. Its first exon is specific to this variant, which has a unique 5' UTR. Isoform 1 has a distinct N-terminus; the remainder of the protein is identical to isoforms 2 and 3. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200472 | GCK (Myc-DDK-tagged)-Human glucokinase (hexokinase 4) (GCK), transcript variant 1 |
CNY 3,656.00 |
|
| RC200472L1 | Lenti ORF clone of Human glucokinase (hexokinase 4) (GCK), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200472L2 | Lenti ORF clone of Human glucokinase (hexokinase 4) (GCK), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC200472L3 | Lenti ORF clone of Human glucokinase (hexokinase 4) (GCK), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200472L4 | Lenti ORF clone of Human glucokinase (hexokinase 4) (GCK), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG200472 | GCK (tGFP-tagged) - Human glucokinase (hexokinase 4) (GCK), transcript variant 1 |
CNY 5,256.00 |
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