DNMT3L (BC002560) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	cytosine-5-methyltransferase 3-like protein; DNA (cytosine-5-)-methyltransferase 3-like; human cytosine-5-methyltransferase 3-like protein; MGC1090
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC002560 edited GTGGGCCTGGATCCTTCCAGCTCATTCTTTGCCTGCGCCGTCCCTCGTTCCATGGCCCAG TCCTCCCCGGGGACCCTGAGCCTGGAAGCCCCGGACCACTGGAACCTTGAACCCACCAGC TGGCTGTACCCGGAGCCGTGGCAGCAGCCCTCATCCCCATGGCGGCCATCCCAGCCCTGG ACCCAGAGGCCGAGCCCAGCATGGACGTGATTTTGGTGGGATCCAGTGAGCTCTCAAGCT CCGTTTCACCCGGGACAGGCAGAGATCTTATTGCATATGAAGTCAAGGCTAACCAGCGAA ATATAGAAGACATCTGCATCTGCTGCGGAAGTCTCCAGGTTCACACACAGCACCCTCTGT TTGAGGGAGGGATCTGCGCCCCATGTAAGGACAAGTTCCTGGATGCCCTCTTCCTGTACG ACGATGACGGGTACCAATCCTACTGCTCCATCTGCTGCTCCGGAGAGACGCTGCTCATCT GCGGAAACCCTGATTGCACCCGATGCTACTGCTTCGAGTGTGTGGATAGCCTGGTCGGCC CCGGGACCTCGGGGAAGGTGCACGCCATGAGCAACTGGGTGTGCTACCTGTGCCTGCCGT CCTCCCGAAGCGGGCTGCTGCAGCGTCGGAGGAAGTGGCGCAGCCAGCTCAAGGCCTTCT ACGACCGAGAGTCGGAGAATCCCCTTGAGATGTTCGAAACCGTGCCTGTGTGGAGGAGAC AGCCAGTCCGGGTGCTGTCCCTTTTTGAAGACATCAAGAAAGAGCTGACGAGTTTGGGCT TTTTGGAAAGTGGTTCTGACCCGGGACAACTGAAGCATGTGGTTGATGTCACAGACACAG TGAGGAAGGATGTGGAGGAGTGGGGACCCTTCGATCTTGTGTACGGCGCCACACCTCCCC TGGGCCACACCTGTGACCGTCCTCCCAGCTGGTACCTGTTCCAGTTCCACCGGCTCCTGC AGTACGCACGGCCCAAGCCAGGCAGCCCCGGGCCCTTCTTCTGGATGTTCGTGGACAATC TGGTGCTGAACAAGGAAGACCTGGACGTCGCATCTCGCTTCCTGGAGATGGAGCCAGTCA CCATCCCAGATGTCCACGGCGGATCCTTGCAGAATGCTGTCCGCGTGTGGAGCAACATCC CAGCCATAAGGAGCAGGCACTGGGCTCTGGTTTCGGAAGAAGAATTGTCCCTGCTGGCCC AGAACAAGCAGAGCTCGAAGCTCGCGGCCAAGTGGCCCACCAAGCTGGTGAAGAACTGCT TTCTCCCCCTAAGAGAATATTTCAAGTATTTTTCAACAGAACTCACTTCCTCTTTATAAA TGAGTCACTATACTGTGAAGAAAAAGACTTTTCCTAGAACAAAGAAAAAAAAAAAAAAAA AAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC002560 unedited NGGTAAAGTCATACTTTGTAATAGAATCCTATAGGCGGCCCGCGAATTCGCACGAGGTGG GCCTGGCTCCTTCCAGCTCATTCTTTGCCTGCGCCGTCCCTCGTTCCATGGCCCAGTCCT CCCCGGGGACCCTGAGCCTGGAAGCCCCGGACCACTGGAACCTTGAACCCACCAGCTGGC TGTACCCGGAGCCGTGGCAGCAGCCCTCATCCCCATGGCGGCCATCCCAGCCCTGGACCC AGAGGCCGAGCCCAGCATGGACGTGATTTTGGTGGGATCCAGTGAGCTCTCAAGCTCCGT TTCACCCGGGACAGGCAGAGATCTTATTGCATATGAAGTCAAGGCTAACCAGCGAAATAT AGAAGACATCTGCATCTGCTGCGGAAGTCTCCAGGTTCACACACAGCACCCTCTGTTTGA GGGAGGGATCTGCGCCCCATGTAAGGACAAGTTCCTGGATGCCCTCTTCCTGTACGACGA TGACGGGTACCAATCCTACTGCTCCATCTGCTGCTCCGGAGAGACGCTGCTCATCTGCGG AAACCCTGATTGCACCCGATGCTACTGCTTCGAGTGTGTGGATAGCCTGGTCGGCCCCGG GACCTCGGGGAAGGTGCACGCCATGAGCAACTGGGTGTGCTACCTGTGCCTGCCGTCCTC CCGAAGCGGGCTGCTGCAGCGTCNGAAGAAGTGGCGCAGCCAGCTCAAGGCCTTCTACGA CCGAGAGTCGGAGAATCCCCTTGAGATGTTCGAAACCGTGCCTGTGTGGAGGAGACAGCC AGTCCGGGTGCTGTCCCTTTTTGAAAGACTCAAAAAGAGCTGACGAGTTTGGGCTTTTTG NAAAGTGGTTCTGACCCGGGACAACTGAAGCATGTGGGTGATGTCACAGACACATGAGGA AAGAGTA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC002560 unedited NGACCATTTGGNTGATGGCAACTTCCCAGGTCCAGGNANAGCACTGGGGNAGGGTCACAG GGCTGCCACCCGGGTATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAGTC GAGTTTTTTTTTTTTTTTTTTTTTTTTCTTTGTTCTAGGAAAAGTCTTTTTCTTCACAGT ATAGTGACTCATTTATAAAGAGGAAGTGAGTTCTGTTGAAAAATACTTGAAATATTCTCT TAGGGGGAGAAAGCAGTTCTTCACCAGCTTGGTGGGCCACTTGGCCGCGAGCTTCGAGCT CTGCTTGTTCTGGGCCAGCAGGGACAATTCTTCTTCCGAAACCAGAGCCCAGTGCCTGCT CCTTATGGCTGGGATGTTGCTCCACACGCGGACAGCATTCTGCAAGGATCCGCCGTGGAC ATCTGGGATGGTGACTGGCTCCATCTCCAGGAAGCGAGATGCGACGTCCAGGTCTTCCTT GTTCAGCACCAGATTGTCCACGAACATCCAAAAAAAGGGCCCGGGGCTGCCTGGCTTGGG CCGTGCGTACTGCAGGAGCCGGTGGAACTGGAACAGGTACCAGCTGGGAGGACGGTCACA GGTGTGGCCCAGGGGAGGTGTGGCGCCGTACACAAGATCGAAGGGTCCCCACTCCTCCAC ATCCTTCCTCACTGTGTCTGTGACATCAACCACATGCTTCAGTTGTCCCGGGTCAGAACC ACTTTTCAAAAAGCCCAAACTCGTCAGCTCTTTCTTGATGTCTTCAAAAAGGGACAGCAC CCGGACTGGCTGTCTCCTCCCACAGGCACGGGTTTCGAACATCTCAAGGGGATTCTCCCA CTCTCGGTCGTAAAAGGCCTTGAGCTGGCTGCNGCATTTCTTCCGAGCTGCACANCCCGC TTCGGGAGGACGGCGGCACAGGTACC
Restriction Sites	Please inquire
ACCN	BC002560
Insert Size	1397 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC002560.2, AAH02560.1
RefSeq Size	1388 bp
Locus ID	29947
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Cysteine and methionine metabolism, Metabolic pathways
Gene Summary	CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

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