RNF181 (BC002803) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HSPC238
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC002803 edited GGCTGTGTCAGAAGGCTGGGCAGCCATGGCGTCCTATTTCGATGAACACGACTGCGAGCC GTCGGACCCTGAGCAGGAGACGCGAACCAACATGCTGCTGGAGCTCGCAAGGTCACTTTT CAATAGGATGGACTTTGAAGACTTGGGGTTGGTAGTAGATTGGGACCACCACCTGCCTCC ACCAGCTGCCAAGACTGTGGTTGAGAACCTCCCCAGGACAGTCATCAGAGGCTCTCAGGC TGAGCTCAAGTGCCCCGTGTGTCTTTTGGAATTTGAGGAGGAGGAGACTGCCATTGAGAT GCCTTGCCATCACCTTTTCCATTCCAGCTGCATTCTGCCCTGGCTAAGCAAGACAAATTC CTGTCCCTTGTGCCGCTATGAGCTGCCCACTGATGACGACACTTATGAGGAGCACAGACG AGATAAGGCTCGAAAACAGCAGCAGCAACACCGACTGGAGAACCTCCATGGAGCCATGTA CACGTGAGGAGGTTGGGGCTGAGTGCTGGCCCTCTGCGTCTTCCTTATTAACCTTGAATC CTCATTAAAGGTTTCTTTACCCACAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC002803 unedited NNGGAACCGGTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACNGGGGC TGTGTCAGAAGGCTGGGCAGCCATGGCGTCCTATTTCGATGAACACGACTGCGAGCCGTC GGACCCTGAGCAGGAGACGCGAACCAACATGCTGCTGGAGCTCGCAAGGTCACTTTTCAA TAGGATGGACTTTGAAGACTTGGGGTTGGTAGTAGATTGGGACCACCACCTGCCTCCACC AGCTGCCAAGACTGTGGTTGAGAACCTCCCCAGGACAGTCATCAGAGGCTCTCAGGCTGA GCTCAAGTGCCCCGTGTGTCTTTTGGAATTTGAGGAGGAGGAGACTGCCATTGAGATGCC TTGCCATCACCTTTTCCATTCCAGCTGCATTCTGCCCTGGCTAAGCAAGACAAATTCCTG TCCCTTGTGCCGCTATGAGCTGCCCACTGATGACGACACTTATGAGGAGCACAGACGAGA TAAGGCTCGAAAACAGCAGCAGCAACACCGACTGGAGAACCTCCATGGAGCCATGTACAC GTGAGGAGGTTGGGGCTGAGTGCTGGCCCTCTGCGTCTTCCTTATTAACCTTGAATCCTC ATTAAAGGTTTCTTTACCCACAAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGC GGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCC AGTGCCTCTCCTGGCCCTGGAAAGTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAA ATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAAATTATGGGTGAGGGGGGG GGGGTTTTTGGANANGGGGCAATTTTTGGGAAAACACCCTGAGGGCCGGGGGGGTTCTAT GGGGACCAAAGCTGAATA
Restriction Sites	Please inquire
ACCN	BC002803
Insert Size	596 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC002803.2, AAH02803.1
RefSeq Size	587 bp
Locus ID	51255
Protein Families	Druggable Genome
Gene Summary	RNF181 binds the integrin alpha-IIb (ITGA2B; MIM 607759)/beta-3 (ITGB3; MIM 173470) complex and has E3 ubiquitin ligase activity (Brophy et al., 2008 [PubMed 18331836]).[supplied by OMIM, Dec 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.