ANAPC13 (BC005398) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	anaphase promoting complex subunit 13; APC13; cyclosome subunit 13; DKFZp566D193; homolog of yeast Swm1; SWM1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC005398 edited GCAGGAGCAAGCGTCTGCCGCGGTGGCCGGGTGCCGATTTGACAAGATCAAAGCTGCAGG AAAATGGACAGTGAGGTTCAGAGAGATGGAAGGATCTTGGATTTGATTGATGATGCTTGG CGAGAAGACAAGCTGCCTTATGAGGATGTCGCAATACCACTGAATGAGCTTCCTGAACCT GAACAAGACAATGGTGGCACCACAGAATCTGTCAAAGAACAAGAAATGAAGTGGACAGAC TTAGCCTTACAGTACCTCCATGAGAATGTTCCCCCCATTGGAAACTGACGCTTGGCTCCT TTCTTGTGGATGGATTTTCTCAAAGTACACAGATAAAGCACGGTTTGTTTCAGTCTCCAA ATTCAAACCTTTGAGTAATAAATCAGCACTCAAAAATGTACACCCATTTAGTTTGTGGTA GCAAAGTGCAATGCGAAATTGAATGAGAAACTGAGATTTCTCAGTAATGGTGAATATTTC GCTCTTTAAACCTAAAACTCTTCATTGAGTAGCTTATATTTGAACATGATTGGTTAAACA TTTGCCTCTACCTCTGATTTTGCTTTGCTGTCAAAGTTTAACACCTTCCAACTACTTATG TGTGTCCTGTAACACAGGTGATTGAACGTATGAGAGGGAAAGGCAAAGAAAAAGGAAGCC AGACACTAGGAGAATTATTAACTTCTCATACTTCCCCACATTGAGAAGCATTCGGAGTGT ATTTAGCCTGTAGATGTTGTGATATGCAAATATCCCATTCCCTGGTTACTGGCATTCCTA AGATTCTTCATGGTATTTTCAAACTTTGGATAAATTTACAGATTAGAAAGATATCTGACA GTTAATCTCTGTTCTCCTTACAAATTCCTTTTGTGCTGCTGGAAAGGATCTTTGGCTAGG TGGATGACTAGTTTTATTCAAAGCCTTTTCTCAAAGCCCTTTCAGTTACAACCACCCCAC TATGGAATCAGTATTTAGTTATACATTTGTATAAGAACCTGTATTTTGAAAAACACATTC ATGTATATTTATTCCTGGAATTATTTGCCTGTTAAACAGTGTCTTTCATGTTCTCTCCCC AGATTGTAAACTCTGTAAGAAGCTGCTTGTATCTGTATCCCTTGTTGAAACTCTGAAAAC ACTGAATAACTAAAATCTCTTCTCATCCTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA A 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC005398 unedited AGTCAGAATATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCCATTACGGCCGGG GGCAGGAGCAAGCGTCTGCCGCGGTGGCCGGGTGCCGATTTGACAAGATCAAAGCTGCAG GAAAATGGACAGTGAGGTTCAGAGAGATGGAAGGATCTTGGATTTGATTGATGATGCTTG GCGAGAAGACAAGCTGCCTTATGAGGATGTCGCAATACCACTGAATGAGCTTCCTGAACC TGAACAAGACAATGGTGGCACCACAGAATCTGTCAAAGAACAAGAAATGAAGTGGACAGA CTTAGCCTTACAGTACCTCCATGAGAATGTTCCCCCCATTGGAAACTGACGCTTGGCTCC TTTCTTGTGGATGGATTTTCTCAAAGTACACAGATAAAGCACGGTTTGTTTCAGTCTCCA AATTCAAACCTTTGAGTAATAAATCAGCACTCAAAAATGTACACCCATTTAGTTTGTGGT AGCAAAGTGCAATGCGAAATTGAATGAGAAACTGAGATTTCTCAGTAATGGTGAATATTT CGCTCTTTAAACCTAANACTCTTCATTGAGTAGCTTATATTTGAACATGATTGGTTAAAC ATTTGCCTCTACCTCTGATTTTGCTTTGCTGTCAAAGTTTAACACCTTCCAACTACTTAT GTGTGTCCTGGTACACAGGTGATTGAACGTATGAGAGGGGAAGGCANNAGAAAAGGAGCC AGACACTAGGAGAATTATTAACTTCTCATACTTCCCCACATTTGAGAGCATTCCGAGTGT ATTTAGCCTGTAGATGTTGTGATATGCAAATATCCATTCCCTGGNTACTGGCATTCCCTA GATTCTTCATGTAATTTTCAACTTTTGGATAATTTACCGATTANAAGGAATTCTGACAGT AAACTCTGGTCTCCTACAATTCCTTTGG
Restriction Sites	Please inquire
ACCN	BC005398
Insert Size	1201 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005398.1, AAH05398.1
RefSeq Size	1201 bp
Locus ID	25847
Protein Pathways	Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
Gene Summary	This gene encodes a component of the anaphase promoting complex, a large ubiquitin-protein ligase that controls cell cycle progression by regulating the degradation of cell cycle regulators such as B-type cyclins. The encoded protein is evolutionarily conserved and is required for the integrity and ubiquitin ligase activity of the anaphase promoting complex. Pseudogenes and splice variants have been found for this gene; however, the biological validity of some of the splice variants has not been determined. [provided by RefSeq, Nov 2008]

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