Calpain 3 (CAPN3) (BC007810) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	calpain, large polypeptide L3; calpain 3; calpain p94, large [catalytic] subunit; CANP3; CANPL3; LGMD2; LGMD2A; MGC4403; MGC10767; MGC11121; MGC14344; muscle-specific calcium-activated neutral protease 3; nCL-1; OTTHUMP00000161194; OTTHUMP00000161196; p94
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC007810 edited GGCACGAGGCACAGGCGATTGGTTTTAGTGGTAGGTGTGTGGGGATCTGTTCTGGTCATC TGGATGCTGGTCATCGGTGTGCAGTATTGATCAGGACCTGCAAACCCAAAAGCTTATGGG AGCTGGCACGTCACAAAAAGAAAAAAACCAAGCCACAGCCTGGCAGCTCTGATCAGGAAA GTGAGGAACAGCAACAATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGACATGGAGA TCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGA AGACACACGGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATG GCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGC AGAAAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGC GAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACATCATTACCA TGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTA GGCTGGAGGGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATGGAGATGGTATCATCA AGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGAACCAGGCTGGCCTCAT CCAAAGCCATGCAGGATCACTCAGGATTTCAGTTTCACCCTCTATTTTCAAAGCCATTTA CCTCAAAGGACCCAGCAGCTACACCCCTACAGGCTTCCAGGCACCTCATCAGTCATGTTC CTCCTCCATTTTACCCCCTACCCATCCTTGATCGGTCATGCCTAGCCTGACCCTTTAGTA AAGCAATGAGGTAGGAAGAACAAACCCTTGTCCCTTTGCCATGTGGAGGAAAGTGCCTGC CTCTGGTCCGAGCCGCCTCGGTTCTGAAGCGAGTGCTCCTGCTTACCTTGCTCTAGGCTG TCTGCAGAAGCACCTGCCGGTGGCACTCAGCACCTCCTTGTGCTAGAGCCCTCCATCACC TTCACGCTGTCCCACCATGGGCCAGGAACCAAACCAGCACTGGGTTCTACTGCTGTGGGG TAAACTAACTCAGTGGAATAGGGCTGGTTACTTTGGGCTGTCCAACTCATAAGTTTGGCT GCATTTTGAAAAAAGCTGATCTAAATAAAGGCATGTGTATGGCTGGAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC007810 unedited NAGTTCAGAATTTGTATACGACTCATATAGGCGGCACGCGAATTCGCACGAGGCACAGGC GATTGGTTTTAGTGGTAAGTGTGTGGGGATCTGTTCTGGTCATCTGGATGCTGGTCATCG GTGTGCAGTATTGATCAGGACCTGCAAACCCAAAAGCTTATGGGAGCTGGCACGTCACAA AAAGAAAAAAACCAAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACA ATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGTGCAGATGAGCT CAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCAC ACTGGAGTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAA CCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCAAACA CTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGA CGCAGGATTCCACCTCAACAACCAGCTCTATGACATCATTACCATGCGGTACGCAGACNA ACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGANGGCATGTT CAGAGCTTTTCATGCATTTGANCAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGA GTGGCTGCAGCTCACCATGTATGCCTGAACCANGCTGGCCTCATCCAAAGCCATGCAGGA TCACTCAGGATTNCAGTTTCACCCTCTATTTTCAAGCCATTNNACTCANAGACCCAGCAG CTACACCCCTACAGCTTCCAGGCCCTCATCAGTCATGTGCCTNCTNCATTTTACCCCTAC CATCCTGATCGGC
Restriction Sites	Please inquire
ACCN	BC007810
Insert Size	1287 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC007810.1, AAH07810.1
RefSeq Size	1287 bp
Locus ID	825
Protein Families	Druggable Genome, Protease
Gene Summary	Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq, Jul 2008]

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