HIST1H2BN (BC011372) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	H2B/d; H2BFD
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC011372 edited GTTGACAGAGCTACCGTCTTCCTGTTTTTTTCCTCCAATTTTCCGGCAGTTACTCCCAGT CATGCCCGAGCCCTCAAAGTCCGCTCCTGCCCCGAAGAAAGGCTCCAAGAAGGCAGTGAC AAAGGCCCAGAAGAAGGACGGCAAGAAGCGCAAGCGCAGCCGCAAGGAGAGCTACTCCGT GTACGTGTACAAGGTGCTGAAGCAGGTCCACCCCGACACCGGTATCTCGTCCAAGGCCAT GGGCATCATGAACTCCTTCGTCAATGACATCTTCGAGCGCATCGCCGGCGAGGCTTCCCG CCTGGCGCATTACAACAAGCGCTCGACCATCACCTCCAGGGAGATCCAGACGGCCGTGCG CCTGCTGCTGCCAGGGGAGCTGGCCAAGCACGCGGTGTCGGAGGGCACCAAGGCCGTCAC CAAGTACACCAGTTCCAAGAAGAGAAAAAGAAGATTCACAGAGAGCATTAAGAAGGCCCA TGGATTCAGAAAGCTCAAGATTTGGCTAAAATTAAGAGTCAGCAACCAATCTCCAGATGA CATTTATATTGCAAGAGATTGAATGCAGAAAGAAGAATTCAGCTTTCTTTTATTAAACAA GGCATTAGAGAGATTTGCAAAATTGTAAAACAGTGTCACTCTTTTTATTTTAAAAAATAG CTATTTTTCATAAAATATGTATGCTAACATAAAATGGGTTTATTACTGTTATTTTTAAAT GAATTAACCAAATTTTTTTGTTTTACTATATAATACGGTAAATGTCAATAGTTATAACCT ACAGAAACACAAATTCTTTGGATTGCAAAGCTCCACCACCTAATATGCTATAGATCAAGG TTCAAAAGAGAGCTCCCTGGAAAACTCCTTGGTGTTTTGTTCACTGAAATATTATTTAAG GAGAACATATTTTATTTTAATGACTGTCATTGCTGGCATCCGAACTCTAATTCCCAAGGC ATCCTCAAATTATCATCTGCATCACTTCATTACTTTTTAAGAGTGTAAGTGGGTCATGAG ACCAGAAGGTTCAAGAACTGCTGCCTTAGGGTATATACCACACTAGTAATGTTCATTTGT GTTCAAATGACTATTTTTCAAAGTATTTGGAGTATCCCTTCCTGCACACTTATGTTATGA TCTAGATATACAGATCCATTTACTTTGTTTTGCTGTTGATGTCTAGGATTGGACTTTTTA ATGTTTGGTTCTATCATTACATAAAATATTTTTACATAGTCCCAAGGTCAAATCAAATTA TATCCAAAGAAATCTTTCTCCTTCATGCTAATAGCCTCTCTTGCCTTAGAGTAATAATTT TAAAGGTTATGATATATCCTTCTGTTGTTTATTATAAGCATATATGTATGTATTCTTAGG GAATATTAACTTCATTTTGCTGTTGATGTCTAGGGATTGGACTTTTTAATATTTGTTTCT ACCGTTACATAAAATATTTTTACATAGTCCCAAGGTCAAATCAAATTATATCCAAAGAAA TCTTTCTCCTTCATCCTAATAGCCTCTCTTACCTTAGAGTAATAATTTTAAATGTTATGA TATATCCTTCTGTTGTTTATTATAAGCATATATATATGTATTCTAGGGAAGATTAACTCG TATCACAGGAGAGGAGACTGGAAGTTGATACTATGCAAGATAAACTTTGTCACAGACAGT CACCTGTTCTTTGGAGGGCCCATTCATCTTTCCCAAAAATAATTTCCTCTCCCCTACATT GCCTACATCCCCCTTCCTGTCTTCCCTATGAAGACAGTATATAAGCTTCTAGATCTCACT GGGCTTGGGGGGCATTCACTTATTTTTCATGTGATACCTCCATGCACAAAATAAAATTTT TTTTTTTCGAGACGGAGTCTTGCCGCGTCTCCCAGGCTGGAGTGCAGTGGCGCGATCTCG GCTCACTGCAAGCTCCGCCTCCGGGGTTTAGGCCATTCTCCTGCCCCAGCCTCCTGAGTA GCTGGGACTACAGGCGCCCGCCACCATGCCCGGCTAATTTTTTTTGTGTTTTCAGTACCG ATGGGGTTTCACTGTGTTAGCTAGGATGGTCTCGATCTCCTGACCTCGTGATCCGCCCGC CTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCACGCCCGGCCACAAAATAA ATTTTTATATCTTTCTCTTATTAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC011372 unedited NCCAGTTCAGATTTGTATACGACTCATATAGGGCGGCCGCGATTCCCGGGATATCGTCGA CCCACGCGTCCGCCCACGCGTCCGGTTGACAGAGCTACCGTCTTCCTGTTTTTTTCCTCC AATTTTCCGGCAGTTACTCCCAGTCATGCCCGAGCCCTCAAAGTCCGCTCCTGCCCCGAA GAAAGGCTCCAAGAAGGCAGTGACAAAGGCCCAGAAGAAGGACGGCAAGAAGCGCAAGCG CAGCCGCAAGGAGAGCTACTCCGTGTACGTGTACAAGGTGCTGAAGCAGGTCCACCCCGA CACCGGTATCTCGTCCAAGGCCATGGGCATCATGAACTCCTTCGTCAATGACATCTTCGA GCGCATCGCCGGCGAGGCTTCCCGCCTGGCGCATTACAACAAGCGCTCGACCATCACCTC CAGGGAGATCCAGACGGCCGTGCGCCTGCTGCTGCCAGGGGAGCTGGCCAAGCACGCGGT GTCGGAGGGCACCAAGGCCGTCACCAAGTACACCAGTTCCAAGAAGAGAAAAAGAAGATT CACAGAGAGCATTAAGAAGGCCCATGGATTCAGAAAGCTCAAGATTTGGCTAAAATTAAG AGTCAGCAACCAATCTCCAGATGACATTTATATTGCAAGAGATTGAATGCAGAAAGAAGA ATTCAGCTTTCTTTTATTAAACAAGGCATTAGAGAGATTTGCAAAATTGTAAAACAGTGT CACTCTTTTTATTTTAAAAAATAGCTATTTTTCATAAAATATGTATGCTAACATAAAATG GGTTTATTACTGTTATTTTTAAATGAATTAACCAAATTTTTTTGTTNTACTATATAATAC GGTAAATGTCAATAGTTATAACCTACAGAAACACAAATCTTTGGATGCAAAGCTCCCCAC CTAATATGCTT
Restriction Sites	Please inquire
ACCN	BC011372
Insert Size	2200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC011372.1
RefSeq Size	2200 bp
Locus ID	8341
Protein Pathways	Systemic lupus erythematosus
Gene Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

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