IGF1 (BC012881) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC012881 edited GAGCCACCTCCCTGAGGCCGCTGCATCGCTTCCTTACAGCAGTCAACCACAGACTTTAAT TAATACAATAAGGTACAGACAACAGGGTGCCACGGACATTTAGGCCCAAGGAATATTATG CAAAAGCAGTGTTTTAAGGGAGTTTCACGTGACAGACCAGCAGATTACACTGAACAAAGA AACAAGAAGTGATGTTGTCCCTGCCTGGAAGTGAACTTGGGGAGGAGAGAGGGAGAATTA TTCACAAAATCTCCTTCCCCAAACAGTCAACACCCAGAACCAACATGGAGCTCCTACCCC ACTGGGACCTGCTGACTGAATTCTTTCTGAGACCCAGTAAGGAGAACTGGTTCCGGCAGC TGAGAAGCCAGGCTGTCTGCTAACGGCCTACAAGGGGCTCTCTCGCTGCTGTTAGGGCTG GTCTGCTCTGTGTCCGTGGTCCAGTTGGGTACAACAAACCCATTCACTCAACAGTGCCAG AGACCCCTCTGGAGACTGGGGAAGCAGCTTCCTTGTGGTCGAGAGCTGTGCAGCCGGGAC AGGAGCCTGCATTCGTTATCACACATCACGGCCTTCGGCTTGGCTGGGGGCCAGGTGGGC ATGGATGCTGGCATGCCCACAGGCAGATCTCAGCTTTCCAGTGAGGTAGCAGCCTGCCGC ACCAAGACTCAGGGCTGTCATGAGGAAGAGCCCCTGGGACCCCCAGCATCCTCGCGAACT CCCTGGAGCTTCATCATGGCCCATGCGCAGCACACGGCAGACAACCGGTTCAGCAGTCCC TGGAGCCCACGTGCCTCCCCTTCCTTTTGCCTTGGCTCCCTTATGCCCACCCACCAGCTC TTCCAGGCTCCAAAGGGGAAGTTGAAGAAGAATCTGGTACCTGGCCCAGCTGCCAAGCCC CAAGGTCACACAGCCCTAGAACCCTGCTGCCAAGTCCGTCTCGGGGGACCTAGGAGAGGA AGTCCGCCAGCCTGGGAAGCTGCCTCTCCAGCCTTGGCCAAGCACCACCTGGCAAACTGC AGCTGAAATGCCGAGCACACTACAGGGGGATACCCCCTCCACCCCAGCCCTGCTGAGAAG CCCAGCCACACTCTTGATCTCCCTGAAATCCTTGAATCTAGGTCAAGGGTGGTGCTAGGG GTTGGATGCACAGGGAAGAGGCTGAGCACCAAGACATGTGGACCAGTCAGGACTAGAGAC CTCCTTCCTTCCAGACATGCACACTCAGCCACGCTGGAGATGACAAAACCTTACACACTC AAAGCCAATACATGCGTAGGGGCGGCCACATGCCACGCACTGTTCTAAATATCTTGTACA AACTCAGCTAATCCTCAGGACAATTCTCATTTTACAGATGAGGAGACCGAGGCACAGCGT GGTGAAGTGACTTGTTCATGGTCACATAGGTGGTAAGGCAGTGAGGTGGAACTTGAACCC AGACAAGCAGCCTCCACTCCCCCCACCTGCTCACCTCTAACTCAGGCCGCTCTTCGAAGA CAACCTGAAGATTTTTCTTTGCAATCGATAGTCTAGAGCAGGAGTCTCCAAGGGGAGAGA CCATGTCCCACTGGGAGCCCAAAGCACTCCAGGGAGGGTAGGAAAAAAATATGAGAGCTT TTGTACTGATTTTGTTTCCTCACTCCTGTTTCCTTCTCTCCCCTTGTGGGTGAGGGCGAG TGAGCAGTTGATCATCTCTTAAACCAGGGCTGCCAGAGCCAGAGCCAGAGCCAGAAAATG GAGTGCCTTCCTTCCTTCCTTTCCTTCCTTCCTTTCCTTCCTTCCTTCCTCCCTCCCTCT CTTTTTTTTTGATGGAGTCTAGCTCTGTCGAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC012881 unedited AACCTAAGATTTGTATACGACTTTTTATAGGCGGCCGCGCATTCTTTCTGAAACCCAGTA AGGAGAACTGGTTCCGGCAGCTGAGAAGCCAGGCTGTCTGCTAACGGCCTACAAGGGGCT CTCTCGCTGCTGTTAGGGCTGGTCTGCTCTGTGTCCGTGGTCCAGTTGGGTACAACAAAC CCATTCACTCAACAGTGCCAGAGACCCCTCTGGAGACTGGGGAAGCAGCTTCCTTGTGGT CGAGAGCTGTGCAGCCGGGACAGGAGCCTGCATTCGTTATCACACATCACGGCCTTCGGC TTGGCTGGGGGCCAGGTGGGCATGGATGCTGGCATGCCCACAGGCAGATCTCAGCTTTCC AGTGAGGTAGCAGCCTGCCGCACCAAGACTCAGGGCTGTCATGAGGAAGAGCCCCTGGGA CCCCCAGCATCCTCGCGAACTCCCTGGAGCTTCATCATGGCCCATGCGCAGCACACGGCA GACAACCGGTTCAGCAGTCCCTGGAGCCCACGTGCCTCCCCTTCCTTTTGCCTTGGCTCC CTTATGCCCACCCACCAGCTCTTCCAGGCTCCAAAGGGGAAGTTGAAGAAGAATCTGGTA CCTGGCCCAGCTGCCAAGCCCCAAAGTCACACAGCCCTAGAACCCTGCTGCCAAGTCCGT CTCGGGGGACCTAGGAGAGGAAGTCCGCCAGCCTGAGAAGCTGCCTCTCCAGCCTTGGCC AAGCACCACCTGGCATACTGCAGCTGATATGCCGAGCACACTACAGGGGGGATACCCCCT CCACCCCCAGCCCTGCTGAGAAGCCCAACCACACTCTTGATCTCCCTGAAATCCCTGAAT CTAGGTCAAGGGTGGGTGCTAGGGGTTGGATGCACAGGGAAAAGCTGAGCACCAGACTGG GGGCCAGTCAGGACAGAGAACCTCT
Restriction Sites	Please inquire
ACCN	BC012881
Insert Size	1832 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC012881.1
RefSeq Size	1832 bp
Locus ID	3479
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways	Dilated cardiomyopathy, Focal adhesion, Glioma, Hypertrophic cardiomyopathy (HCM), Long-term depression, Melanoma, mTOR signaling pathway, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer
Gene Summary	The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]

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