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LIM kinase 2 (LIMK2) (BC013051) Human Untagged Clone
CAT＃: SC123647
LIMK2 (untagged)-Human LIM domain kinase 2 (cDNA clone MGC:8917 IMAGE:3861412), complete cds
CNY 12,144.00
货期*
现货
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for BC013051 edited
GGGAGGAGCCATCCCAGCCATGAGCCCCTGTGGGAATCTGCTGGGGGCCAAGTGGCCTGG AGTCCTCAGGCTCCCGCAGCTGCTCCGGAGGGAGAGGTGAGCTCAGGGCAGCCTGCCTGC AGCCAGAGGTGCCGGGAGCCCCGGGCCTGTCATGGTGGCCATCTACAGCCGGCCTGAGGC AGTCACAGACGGATTTGCAGCTGAGCCTGTCTATCTGGTGTGGGAAGAAGATGGGGAGTT ACTTGTCAGTCCCGGCTTACTTCACCTCCAGAGACCTGTTTCGGTGTTCAGAATGCCAGG ATTCCCTCACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCCAAGGACTACT GGGGGAAGTTTGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACAGGGCCTTTTATGG TGGCTGGGGAGTTCAAGTACCACCCAGAGTGCTTTGCCTGTATGAGCTGCAAGGTGATCA TTGAGGATGGGGATGCATATGCACTGGTGCAGCATGCCACCCTCTACTGTGGGAAGTGCC ACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTCCACAGAGTCTGTTCAGGAGC AGCTGCCCTACTCTGTCACGCTCATCTCCATGCCGGCCACCACTGAAGGCAGGCGGGGCT TCTCCGTGTCCGTGGAGAGTGCCTGCTCCAACTACGCCACCACTGTGCAAGTGAAAGAGG TCAACCGGATGCACATCAGTCCCAACAATCGAAACGCCATCCACCCTGGGGACCGCATCC TGGAGATCAATGGGACCCCCGTCCGCACACTTCGAGTGGAGGAGGTGGAGGATGCAATTA GCCAGACGAGCCAGACACTTCAGCTGTTGATTGAACATGACCCCGTCTCCCAACGCCTGG ACCAGCTGCGGCTGGAGGCCCGGCTCGCTCCTCACATGCAGAATGCCGGACACCCCCACG CCCTCAGCACCCTGGACACCAAGGAGAATCTGGAGGGGACACTGAGGAGACGTTCCCTAA GGCGCAGTAACAGTATCTCCAAGTCCCCTGGCCCCAGCTCCCCAAAGGAGCCCCTGCTGT TCAGCCGTGACATCAGCCGCTCAGAATCCCTTCGTTGTTCCAGCAGCTATTCACAGCAGA TCTTCCGGCCCTGTGACCTAATCCATGGGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGG CTATCAAGGTGACACACAAAGCCACGGGCAAAGTGATGGTCATGAAAGAGTTAATTCGAT GTGATGAGGAGACCCAGAAAACTTTTCTGACTGAGGTGAAAGTGATGCGCAGCCTGGACC ACCCCAATGTGCTCAAGTTCATTGGTGTGCTGTACAAGGATAAGAAGCTGAACCTCCTGA CAGAGTACATTGAGGGGGGCACACTGAAGGACTTTCTGCGCAGTATGGATCCGTTCCCCT GGCAGCAGAAGGTCAGGTTTGCCAAAGGAATCGCCTCCGGAATGGCCTATTTGCACTCTA TGTGCATCATCCACCGGGATCTGAACTCGCACAACTGCCTCATCAAGTTGGACAAGACTG TGGTGGTGGCAGACTTTGGGCTGTCACGGCTCATAGTGGAAGAGAGGAAAAGGGCCCCCA TGGAGAAGGCCACCACCAAGAAACGCACCTTGCGCAAGAACGACCGCAAGAAGCGCTACA CGGTGGTGGGAAACCCCTACTGGATGGCCCCTGAGATGCTGAACGGAAAGAGCTATGATG AGACGGTGGATATCTTCTCCTTTGGGATCGTTCTCTGTGAGATCATTGGGCAGGTGTATG CAGATCCTGACTGCCTTCCCCGAACACTGGACTTTGGCCTCAACGTGAAGCTTTTCTGGG AGAAGTTTGTTCCCACAGATTGTCCCCCGGCCTTCTTCCCGCTGGCCGCCATCTGCTGCA GACTGGAGCCTGAGAGCAGAGCCCCCCCCGGGGCCGCAGGAGAGGGCCCGGGCTGCGCGG ATGATGAGGGCCCAGTGAGGCGCCAAGGGAAGGTCACCATCAAGTATGACCCCAAGGAGC TACGGAAGCACCTCAACCTAGAGGAGTGGATCCTGGAGCAGCTCACGCGCCTCTACGACT GCCAGGAAGAGGAGATCTCAGAACTAGAGATTGACGTGGATGAGCTCCTGGACATGGAGA GTGACGATGCCTGGGCTTCCAGGGTCAAGGAGCTGCTGGTTGACTGTTACAAACCCACAG AGGCCTTCATCTCTGGCCTGCTGGACAAGATCCGGGCCATGCAGAAGCTGAGCACACCCC AGAAGAAGTGAGGGTCCCCGACCCAGGCGAACGGTGGCTCCCATAGGACAATCGCTACCC CCCGACCTCGTAGCAACAGCAATACCGGGGGACCCTGCGGCCAGGCCTGGTTCCATGAGC AGGGCTCCTCGTGCCCCTGGCCCAGGGGTCTCTTCCCCTGCCCCCTCAGTTTTCCACTTT TGGATTTTTTTATTGTTATTAAACTGATGGGACTTTGTGTTTTTATATTGACTCTGCGGC ACGGGCCCTTTAATAAAGCGAGGTAGGGTACGCCTTTGGTGCAGCTCAAAAAAAAAAAAA AAAATGATTTCCAGCGGTCCACATTAGAGTTGAAATTTTCTGGTGGGAGAATCTATACCT TGTTCCTTTATAGGCCAAGGACCGCAGTCCTTCAGTAACACCAGTGTAAAAGCTTGAGGA GAAATTGTGAAGCTACACAGTATTTGTTTTCTAATACCTCTTGTCATTCTAAATATCTTT AATTTATTAAAAAATATATATATACAGTAAAAAAAAAAAAAAAAA >OriGene 5' read for BC013051 unedited
CCGAATTTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCCCGGGATATCGTCGACC CACGCGTCCGGGGAGGAGCCATCCCAGCCATGAGCCCCTGTGGGAATCTGCTGGGGGCCA AGTGGCCTGGAGTCCTCAGGCTCCCGCAGCTGCTCCGGAGGGAGAGGTGAGCTCAGGGCA GCCTGCCTGCAGCCAGAGGTGCCGGGAGCCCCGGGCCTGTCATGGTGGCCATCTACAGCC GGCCTGAGGCAGTCACAGACGGATTTGCAGCTGAGCCTGTCTATCTGGTGTGGGAAGAAG ATGGGGAGTTACTTGTCAGTCCCGGCTTACTTCACCTCCAGAGACCTGTTTCGGTGTTCA GAATGCCAGGATTCCCTCACCAACTGGTACTATGAGAAGGATGGGAAGCTCTACTGCCCC AAGGACTACTGGGGGAAGTTTGGGGAGTTCTGTCATGGGTGCTCCCTGCTGATGACAGGG CCTTTTATGGTGGCTGGGGAGTTCAAGTACCACCCAGAGTGCTTTGCCTGTATGAGCTGC AAAGTGATCATTGAGGATGGNGATGCATATGCACTGGTGCAGCATGCCACCCTCTACTGT GGGAAGTGCCACAATGAGGTGGTGCTGGCACCCATGTTTGAGAGACTCTCACAGAGTCTG TTCAGGAGCAGCTGCCCTACTTTGTCACGCTCATCTTCATGGCCGGCCACCATGTAGGGG CAGCGGGGCCTCCTCCGGGTCGTGGGAGAGGGCCTGGCTCAACTACCCCACCCCTGGGCA GGTGAAAGAGTCAACCGGAATGCACTTAGTCCCCACAATCGAAACGCCTCCCACCCGGGG ACCCCATCCTGGAAACCAAGGGACCCCCGTCCCCACCTTTCGGGGGAGGAGGTGGGGGGT CAATTAGCCAGAAAGCCCCACACTTAGCCGTGGAGTGAAGAGAAC |
| Restriction Sites | Please inquire |
| ACCN | BC013051 |
| Insert Size | 2805 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC013051.1, AAH13051.1 |
| RefSeq Size | 2805 bp |
| Locus ID | 3985 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
| Gene Summary | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
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