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FZR1 (NM_016263) Human Untagged Clone
CAT＃: SC123643
FZR1 (untagged)-Human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDC20C; CDH1; FZR; FZR2; HCDH; HCDH1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_016263 edited
GCGACCGCCGCCATATTAGGGACCGCGGAGCCCGGGATCCCGTCAGCGGCGGCCGCGGCC GGGCCTGCGGGAGCTGCGGAGGCCGGAGGCGGGCGCTGTGCGGTGCCAGGAGAGGCGGGG TCGGCGGGAGCCAGCGAGCCACGGGAGCGAGCCAGGCTAACCTTGCCGCGGGCCGAGCCC TGCCTCGCCATGGACCAGGACTATGAGCGGCGCCTGCTTCGCCAGATCGTCATCCAGAAT GAGAACACGATGCCACGCGTCACAGAGATGCGGCGGACCCTGACGCCTGCCAGCTCCCCA GTGTCCTCGCCCAGCAAGCACGGAGACCGCTTCATCCCCTCCAGAGCCGGAGCCAACTGG AGCGTGAACTTCCACAGGATTAACGAGAATGAGAAGTCTCCCAGTCAGAACCGGAAAGCC AAGGACGCCACCTCAGACAACGGCAAAGACGGCCTGGCCTACTCTGCCCTGCTCAAGAAT GAGCTGCTGGGTGCCGGCATCGAGAAGGTGCAGGACCCGCAGACTGAGGACCGCAGGCTG CAGCCCTCCACGCCTGAGAAGAAGGGTCTGTTCACGTATTCCCTTAGCACCAAGCGCTCC AGCCCCGATGACGGCAACGATGTGTCTCCCTACTCCCTGTCTCCCGTCAGCAACAAGAGC CAGAAGCTGCTCCGGTCCCCCCGGAAACCCACCCGCAAGATCTCCAAGATCCCCTTCAAG GTGCTGGACGCGCCCGAGCTGCAGGACGACTTCTACCTCAATCTGGTGGACTGGTCGTCC CTCAATGTGCTCAGCGTGGGGCTAGGCACCTGCGTGTACCTGTGGAGTGCCTGTACCAGC CAGGTGACGCGGCTCTGTGACCTCTCAGTGGAAGGGGACTCAGTGACCTCCGTGGGCTGG TCTGAGCGGGGGAACCTGGTGGCGGTGGGCACACACAAGGGCTTCGTGCAGATCTGGGAC GCAGCCGCAGGGAAGAAGCTGTCCATGTTGGAGGGCCACACGGCACGCGTCGGGGCGCTG GCCTGGAATGCTGAGCAGCTGTCGTCCGGGAGCCGCGACCGCATGATCCTGCAGAGGGAC ATCCGCACCCCGCCACTGCAGTCGGAGCGGCGGCTGCAGGGCCACCGGCAGGAGGTGTGC GGGCTCAAGTGGTCCACAGACCACCAGCTCCTCGCCTCGGGGGGCAACGACAACAAGCTG CTGGTCTGGAATCACTCGAGCCTGAGCCCCGTGCAGCAGTACACGGAGCACCTGGCGGCC GTGAAGGCCATCGCCTGGTCCCCACATCAGCACGGGCTGCTGGCCTCGGGGGGCGGCACA GCTGACCGCTGTATCCGCTTCTGGAACACGCTGACAGGACAACCACTGCAGTGTATCGAC ACGGGCTCCCAAGTGTGCAATCTGGCCTGGTCCAAGCACGCCAACGAGCTGGTGAGCACG CACGGCTACTCACAGAACCAGATCCTTGTCTGGAAGTACCCCTCCCTGACCCAGGTGGCC AAGCTGACCGGGCACTCCTACCGCGTGCTGTACCTGGCAATGTCCCCTGATGGGGAGGCC ATCGTCACTGGTGCTGGAGACGAGACCCTGAGGTTCTGGAACGTCTTTAGCAAAACCCGT TCGACAAAGGAGTCTGTGTCTGTGCTCAACCTCTTCACCAGGATCCGGTAAACCTGCCGG GCAGGACCGTGCCACACCAGCTGTCCAGAGTCGGAGGACCCCAGCTCCTCAGCTTGCATG GACTCTGCCTTCCCAGCGCTTGTCCCCCGAGGAAGGCGGCTGGGCGGGCGGGGAGCTGGG CCTGGAGGATCCTGGAGTCTCATTAAATGCCTGATTGTGAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_016263 unedited
AAGGCCCTGAGGTTTTGTTATCCCACTTACTATAGGCGGCCGCGCAATTCGCACGAGGGC GACCGCCGCCATATTAGGGACCGCGGAGCCCGGGATCCCGTCAGCGGCGGCCGCGGCCGG GCCTGCGGGAGCTGCGGAGGCCGGAGGCGGGCGCTGTGCGGTGCCAGGAGAGGCGGGGTC GGCGGGAGCCAGCGAGCCACGGGAGCGAGCCAGGCTAACCTTGCCGCGGGCCGAGCCCTG CCTCGCCATGGACCAGGACTATGAGCGGCGCCTGCTTCGCCAGATCGTCATCCAGAATGA GAACACGATGCCACGCGTCACAGAGATGCGGCGGACCCTGACGCCTGCCAGCTCCCCAGT GTCCTCGCCCAGCAAGCACGGAGACCGCTTCATCCCCTCCAGAGCCGGAGCCAACTGGAG CGTGAACTTCCACAGGATTAACGAGAATGAGAAGTCTCCCAGTCAGAACCGGAAAGCCAA GGACGCCACCTCAGACAACGGCAAAGACGGCCTGGCCTACTCTGCCCTGCTCAAGAATGA GCTGCTGGGTGCCGGCATCGAGAAGGTGCAGGACCCGCAGACTGAGGACCGCAGGCTGCA GCCCTCCACGCCTGAGAAGAAGGGTCTGTTCACGTATTCCCTTAGCACCAAGCGCTCCAG CCCCGATGACGGCAACGATGTGTCTCCCTACTCCCTGTCTCCCGTCAGCAACAAGAGCCA GAAGCTGCTCCGGTCCCCCCGGAAACCCACCCGCAAGATCTCCAAGATCCCCTTCAAGGT GCTGGACGCGCCCGAGCTGCAGGACGACTTCTACCTCAATCTGGTGGACTGGTCGTCCCT CAATGTGCTCAGCGTGGGGCTAGGCACCTGCGTGTACCTGTGNAGTGCCTGTACCCAGCC AGTGATCGCGCTCTGTGC >OriGene 3' read for NM_016263 unedited
GGGGACTTGGGCTTGTATGCACACTCTTGNATCCTCCAGACCCAGCTCCCCGCCCGCCCA GCCGCCTTCCTCGGGGGACAAGCGCTGGGAAGGCAGAGTCCATGCAAGCTGAGGAGCTGG GGTCCTCCGACTCTGGACAGCTGGTGTGGCACGGTCCTGCCCGGCAGGTTTACCGGATCC TGGTGAAGAGGTTGAGCACAGACACAGACTCCTTTGTCGAACGGGTTTTGCTAAAGACGT TCCAGAACCTCAGGGTCTCGTCTCCAGCACCAGTGACGATGGCACTCCCCATCAGGGGAC ATTGCCAGGTACAGCACGCGGTAGGAGTGCCCGGTCAGCTTGGCCACCTGGGTCAGGGAG GGGTACTTCCAGACAAGGATCTGGTTCTGTGAGTAGCCGTGCGTGCTCACCAGCTCGTTG GCGTGCTTGGACCATGCCAGATTGCACACTTGGGAGCCCGTGTCGATACACTGCAGTGGT TGTCCTGTCAGCGTGTTCCAGAAGCGGATACAGCGGTCAGCTGTGCCGCCCCCCGAGGCC AGCAGCCCGTGCTGATGTGGGGACCAGGCGATGGCCTTCACGGCCGCCAGGTGCTCCGTG TACTGCTGCACGGGGCTCAGGCTCGAGTGATTCCAGACCAGCAGCTTGTTGTCGTTGCCC CCCGAGGCGAGGAGCTGGTGGTCTGTGGACCACTTGAGCCCGCACACCTCCTGCCGGTGG CCCTGCAGCCGCCGCTCCGACTGCAGTGGCGGGGTGCGGATGTCCCTCTGCAGGATCATG CNGTCGCGGCTCCCGGACGACAGCTGCTCAGCATTCCAGGCCAGCGCCCCGACGCGTGCC GTGTGGCCCTTCCACATGGACAGCTTTCTTCCTGCGGCTGCGTCCCAGATCTGCACGAAC CCTT |
| Restriction Sites | Please inquire |
| ACCN | NM_016263 |
| Insert Size | 1866 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016263.2, NP_057347.2 |
| RefSeq Size | 5008 bp |
| RefSeq ORF | 1482 bp |
| Locus ID | 51343 |
| UniProt ID | Q9UM11 |
| Domains | WD40 |
| Protein Families | Druggable Genome |
| Protein Pathways | Cell cycle, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis |
| Gene Summary | Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed:25349192).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks a short in-frame segment in the 3' coding region compared to variant 1. The encoded protein (isoform 2) has a shorter C-terminus compared to isoform 1. This isoform is also known as isoform alpha. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201413 | FZR1 (Myc-DDK-tagged)-Human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2 |
CNY 3,656.00 |
|
| RC201413L1 | Lenti ORF clone of Human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201413L2 | Lenti ORF clone of Human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC201413L3 | Lenti ORF clone of Human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201413L4 | Lenti ORF clone of Human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG201413 | FZR1 (tGFP-tagged) - Human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2 |
CNY 5,256.00 |
