TIA1 (BC015944) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	TIA-1; WDM
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC015944 edited GGCGCCGCCGCGACAGCAGCAGCCATGGAGGACGAGATGCCCAAGACTCTATACGTCGGT AACCTTTCCAGAGATGTGACAGAAGCTCTAATTCTGCAACTCTTTAGCCAGATTGGACCT TGTAAAAACTGCAAAATGATTATGGATACAGCTGGAAATGATCCCTATTGTTTTGTGGAG TTTCATGAGCATCGTCATGCAGCTGCAGCATTAGCTGCTATGAATGGACGGAAGATAATG GGTAAGGAAGTCAAAGTGAATTGGGCAACAACCCCTAGCAGTCAAAAGAAAGATACAAGC AGTAGTACCGTTGTCAGCACACAGCGTTCACAAGATCATTTCCATGTCTTTGTTGGTGAT CTCAGCCCAGAAATTACAACTGAAGATATAAAAGCTGCTTTTGCACCATTTGGAAGAATA TCAGATGCCCGAGTGGTAAAAGACATGGCAACAGGAAAGTCTAAGGGATATGGCTTTGTC TCCTTTTTCAACAAATGGGATGCTGAAAACGCCATTCAACAGATGGGTGGCCAGTGGCTT GGTGGAAGACAAATCAGAACTAACTGGGCAACCCGAAAGCCTCCCGCTCCAAAGAGTACA TATGAGTGTAGGTGTATTGGAGAAGAAAAGGAAATGTGGAATTTTGGAGAAAAATACGCT AGATTTTAAATGTTAGAGCTGTTCCCGGAGACTTATTGCAGAAATAGATGAGAAGCAAAT CAAGACTACTATTCAAAAATGTACTTAGTTTTCATTTTTGTAATTATAAATAATATTATT TCTAATGTCAAGTCTCCTATTAAATAGAAAATACTGGGTAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC015944 unedited NNGGTCAGAATTTGTATACGACTCATATAGGCGGCCGCGNAATCCCGGGATATCGTCGAC CCACGCGTCCGGGCGCCGCCGCGACAGCAGCAGCCATGGAGGACGAGATGCCCAAGACTC TATACGTCGGTAACCTTTCCAGAGATGTGACAGAAGCTCTAATTCTGCAACTCTTTAGCC AGATTGGACCTTGTAAAAACTGCAAAATGATTATGGATACAGCTGGAAATGATCCCTATT GTTTTGTGGAGTTTCATGAGCATCGTCATGCAGCTGCAGCATTAGCTGCTATGAATGGAC GGAAGATAATGGGTAAGGAAGTCAAAGTGAATTGGGCAACAACCCCTAGCAGTCAAAAGA AAGATACAAGCAGTAGTACCGTTGTCAGCACACAGCGTTCACAAGATCATTTCCATGTCT TTGTTGGTGATCTCAGCCCAGAAATTACAACTGAAGATATAAAAGCTGCTTTTGCACCAT TTGGAAGAATATCAGATGCCCGAGTGGTAAAAGACATGGCAACAGGAAAGTCTAAGGGAT ATGGCTTTGTCTCCTTTTTCAACAAATGGGATGCTGAAAACGCCATTCAACAGATGGGTG GCCAGTGGCTTGGTGGAAGACAAATCAGAACTAACTGGGCAACCCGAAAGCCTCCCGCTC CAAAGAGTACATATGAGTGTAGGTGTATTGGGAGAAGAAAAGGAAATGTGGAATTTTGGG AGAAAAATACGCTAGATTTTAAATGTTAGAGCTGTTCCCGGAGACTTATTGCAGAAATAG ATGAGAAGCANATCAAGACTACTATTCAAAAATGTACTTAGTTNTCATTTTTGTAATTAT AAATAATATTATTTCTAATGTCAAGTCTCCTATTAAATAGAAAATACTGGGTAAAAAAAA AAAAAGGGCNGCCGCNGTCATAGCTGTTTCTGAACGATCCCG
Restriction Sites	Please inquire
ACCN	BC015944
Insert Size	834 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC015944.1, AAH15944.1
RefSeq Size	834 bp
Locus ID	7072
Protein Families	Druggable Genome
Gene Summary	The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms has been found for this gene. [provided by RefSeq, May 2017]

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