BPNT1 (BC017801) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	3'(2'), 5'-bisphosphate nucleotidase 1; bisphosphate 3'-nucleotidase 1; BPntase; OTTHUMP00000035564; PAP-inositol-1,4-phosphatase; PIP
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC017801 edited ACTTATTGACAGTGCCAAAGCTCGGTACTGGACACAACGAGGGACCTGGGTCTACGATAA CGCGCTTTTGCTCCTCCTGAAGTGTCTTTGGTCCAACGTTGTTCCAGAGTGTACCATGGC TTCCAGTAACACTGTGTTGATGCGGTTGGTAGCCTCCGCATATTCTATTGCTCAAAAGGC AGGAATGATAGTCAGACGTGTTATTGCTGAAGGAGACCTGGGTATTGTGGAGAAGACCTG TGCAACAGACCTGCAGACCAAAGCTGACCGATTGGCACAGATGAGCATATGTTCTTCATT GGCCCGGAAATTCCCCAAACTCACAATTATAGGGGAAGAGGATCTGCCTTCTGAGGAAGT GGATCAAGAGCTGATTGAAGACAGTCAGTGGGAAGAAATACTGAAGCAACCATGCCCATC GCAGTACAGTGCTATTAAAGAAGAAGATCTCGTGGTCTGGGTTGATCCTCTGGATGGAAC CAAGGAATATACCGAAGGTCTTCTTGACAATGTAACAGTTCTTATTGGAATTGCTTATGA AGGAAAAGCCATAGCAGGAGTTATTAACCAGCCATATTACAACTATGAGGCAGGACCAGA TGCTGTGTTGGGGAGGACAATCTGGGGAGTTTTAGGTTTAGGCGCCTTTGGGTTTCAGCT GAAAGAAGTCCCTGCTGGGAAACACATTATCACAACTACTCGATCCCATAGCAACAAGTT GGTTACTGACTGTGTTGCTGCTATGAACCCCGATGCTGTGCTGCGAGTAGGAGGAGTAGG AAATAAGATTATTCAGCTGATTGAAGGCAAAGCCTCTGCTTATGTATTTGCAAGTCCTGG TTGTAAGAAGTGGGATACTTGTGCTCCAGAAGTTATTTTACATGCTGTGGGAGGCAAGTT AACCGATATCCATGGGAATGTTCTTCAGTACCACAAGGATGTGAGCCACCGCACCTGGCC TAAACCAGATTTCTTTAGGGCACAATTTTTTCTGGAATCTCACTCTTGTTTTTCACGTAA TTTTAAAAACGTTTCTACTCCAATTAAGAATATATATGATGTTATTATATATGCTTATGA AACAGATTTATGAGAAAAGTTTTTTTTAAATAAATTATTTAATCAAAAAAAAAAAAAAAA AAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC017801 unedited NGAGATCCTGGATTTGTTATACGATTTTATATAGGCGGCCGCGCAATTCGCCATTACGGC CGGGGATTTATTGACAGTGCCAAAGCTCGGTACTGGACACAACGAGGGACCTGGGTCTAC GATAACGCGCTTTTGCTCCTCCTGAAGTGTCTTTGGTCCAACGTTGTTCCAGAGTGTACC ATGGCTTCCAGTAACACTGTGTTGATGCGGTTGGTAGCCTCCGCATATTCTATTGCTCAA AAGGCAGGAATGATAGTCAGACGTGTTATTGCTGAAGGAGACCTGGGTATTGTGGAGAAG ACCTGTGCAACAGACCTGCAGACCAAAGCTGACCGATTGGCACAGATGAGCATATGTTCT TCATTGGCCCGGAAATTCCCCAAACTCACAATTATAGGGGAAGAGGATCTGCCTTCTGAG GAAGTGGATCAAGAGCTGATTGAAGACAGTCAGTGGGAAGAAATACTGAAGCAACCATGC CCATCGCAGTACAGTGCTATTAAAGAAGAAGATCTCGTGGTCTGGGTTGATCCTCTGGAT GGAACCAAGGAATATACCGAAGGTCTTCTTGACAATGTAACAGTTCTTATTGGAATTGCT TATGAAGGAAAAGCCATAGCAGGAGTTATTAACCAGCCATATTACAACTATGAGGCAGGA CCAGATGCTGTGTTGGGGAGGACAATCTGGGGAGTTTTAGGTTTAGGCGCCTTTGGGTTT CAGCTGANAGAGTCCCTGCTNGGAAACACATTATCACAACTACTCGATCCCATAGCACCA GTTGGTTACTGACTGTGTTGCTGCTATGAACCCCGATGCTGTGCTGCGAGTTAGGAGAGT AGGAAATAAAAATATTCAGCTGATTGGAGGGCAAGCCTCTGCCTATGTATTTGCAAGTCC TGGTTGTAANAAC
Restriction Sites	Please inquire
ACCN	BC017801
Insert Size	1153 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC017801.1, AAH17801.1
RefSeq Size	1153 bp
Locus ID	10380
Protein Pathways	Sulfur metabolism
Gene Summary	BPNT1, also called bisphosphate 3-prime-nucleotidase, or BPntase, is a member of a magnesium-dependent phosphomonoesterase family. Lithium, a major drug used to treat manic depression, acts as an uncompetitive inhibitor of BPntase. The predicted human protein is 92% identical to mouse BPntase. BPntase's physiologic role in nucleotide metabolism may be regulated by inositol signaling pathways. The inhibition of human BPntase may account for lithium-induced nephrotoxicity. [provided by RefSeq, Jul 2008]

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