Probable hydrolase PNKD (PNKD) (BC021118) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	brain protein 17; BRP17; DKFZp564N1362; DYT8; FKSG19; FPD1; KIAA1184; KIPP1184; MGC31943; MR-1; MR1; myofibrillogenesis regulator 1; paroxysmal nonki; paroxysmal nonkinesiogenic dyskinesia; PDC; TAHCCP2; trans-activated by hepatitis C virus core protein 2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC021118 edited GAGCGCGGTGAAGCGGGGGTGGGATCTGAACATGGCGGCGGTGGTAGCTGCTACGGCGCT GAAGGGCCGGGGGGCGAGAAATGCCCGCGTCCTCCGGGGGATTCTCGCAGGAGCCACAGC TAACAAGGCTTCTCATAACAGGACCCGGGCCCTGCAAAGCCACAGCTCCCCAGAGGGCAA GGAGGAACCTGAACCCCTATCCCCGGAGCTGGAATACATTCCCAGAAAGAGGGGCAAGAA CCCCATGAAAGCTGTGGGACTGGCCTGGGCCATCGGCTTCCCTTGTGGTATCCTCCTCTT CATCCTCACCAAGCGGGAAGTGGACAAGGACCGTGTGAAGCAGATGAAGGCTCGGCAGAA CATGCGGTTGTCCAACACGGGCGAGTATGAGAGCCAGAGGTTCAGGGCTTCCTCCCAGAG TGCCCCGTCCCCTGATGTTGGGTCTGGGGTGCAGACCTGAGGAGCGCTGCGACCCTCCTA GGCTATTGACTGTTAAGTCCTCAGGTTTGGCCCAGATTCCAGTTCGTGCCTCTGAGGTCC ACCAGAGGGCGCATGAAGCCCAGGCTGTTGCCAAACCCTACCCTGCCCCACACCAAGGAG CCAGCCAAAGGCAAATAAAGTTATTGAGTGTTTAGTAGAAAGGGAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC021118 unedited TTTNGGNNGACGCTCAGCATTTGTNATCCAACTCACTANAGGGCGGCCGCGCAATTCGCA CGAGGGAGCGCGGTGAAGCGGGGGTGGGATCTGAACATGGCGGCGGTGGTAGCTGCTACG GCGCTGAAGGGCCGGGGGGCGAGAAATGCCCGCGTCCTCCGGGGGATTCTCGCAGGAGCC ACAGCTAACAAGGCTTCTCATAACAGGACCCGGGCCCTGCAAAGCCACAGCTCCCCAGAG GGCAAGGAGGAACCTGAACCCCTATCCCCGGAGCTGGAATACATTCCCAGAAAGAGGGGC AAGAACCCCATGAAAGCTGTGGGACTGGCCTGGGCCATCGGCTTCCCTTGTGGTATCCTC CTCTTCATCCTCACCAAGCGGGAAGTGGACAAGGACCGTGTGAAGCAGATGAAGGCTCGG CAGAACATGCGGTTGTCCAACACGGGCGAGTATGAGAGCCAGAGGTTCAGGGCTTCCTCC CAGAGTGCCCCGTCCCCTGATGTTGGGTCTGGGGTGCAGACCTGAGGAGCGCTGCGACCC TCCTAGGCTATTGACTGTTAAGTCCTCAGGTTTGGCCCAGATTCCAGTTCGTGCCTCTGA GGTCCACCAGAGGGCGCATGAAGCCCAGGCTGTTGCCAAACCCTACCCTGCCCCACACCA AGGAGCCAGCCAAAGGCAAATAAAGTTATTGGAGTGTTTAGTNNGAAAGGAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTCGACTCTAGATTGCGGCCGCGGTCATA GCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCAGTGCCTCTCCTGG CCCTGG
Restriction Sites	Please inquire
ACCN	BC021118
Insert Size	684 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021118.1, AAH21118.1
RefSeq Size	684 bp
RefSeq ORF	429 bp
Locus ID	25953
Protein Families	Transmembrane
Gene Summary	This gene is thought to play a role in the regulation of myofibrillogenesis. Mutations in this gene have been associated with the movement disorder paroxysmal non-kinesigenic dyskinesia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

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