TMLHE (BC025269) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BBOX2; butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 2; epsilon-trimethyllysine 2-oxoglutarate; epsilon-trimethyllysine hydroxylase; FLJ10727; OTTHUMP00000024255; TMLH; trimethyllysine hydroxylase, epsilon; XAP130
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC025269 edited GGCGGGTGAAAGGCTGCCTCCCGAGACTCTCCTTGCTTGGAATTCTGCCCACTCTGCGGA GTTAGCAGTCACGACCTCCAGCACAGGATGTGGTACCACAGATTGTCCCACCTACACAGC AGGCTTCAGGACTTGCTGAAGGGAGGAGTCATATATCCGGCCCTTCCACAGCCCAACTTC AAAAGCTTACTTCCTTTAGCTGTCCATTGGCACCATACAGCCTCCAAGTCTCTGACTTGT GCTTGGCAGCAACATGAAGATCATTTTGAGCTGAAATATGCTAATACCGTGATGCGCTTT GATTACGTCTGGCTTCGAGACCACTGCCGCTCAGCATCGTGCTACAACTCTAAGACTCAC CAGCGCAGCCTGGATACTGCCAGTGTGGATTTATGTATCAAGCCAAAGACCATTCGTCTG GATGAGACCACACTCTTTTTCACTTGGCCAGATGGTCATGTGACTAAATATGATTTGAAT TGGCTGGTGAAAAACAGCTATGAAGGGCAGAAACAAAAAGTCATCCAGCCTAGAATACTA TGGAATGCTGAAATCTACCAGCAAGCCCAAGTTCCATCGGTAGATTGCCAGAGCTTCTTA GAAACCAACGAGGGACTGAAGAAGTTTCTGCAAAACTTTCTGCTCTATGGAATTGCATTC GTAGAAAATGTCCCTCCCACTCAAGAGCACACAGAGAAGTTGGCAGAAAGGATCAGCTTA ATCAGAGAAACCATTTATGGGAGGATGTGGTATTTCACTTCAGACTTCTCCAGAGGTGAC ACTGCGTACACCAAGCTAGCTCTGGATCGGCACACTGACACTACCTATTTTCAAGAGCCC TGTGGCATTCAAGTGTTTCATTGTCTTAAACATGAAGGAACTGGTGGCAGGACACTGCTA GTAGATGGATTCTATGCAGCAGAACAGGTACTTCAAAAGGCACCTGAGGAATTTGAACTC CTCAGTAAAGTGCCATTGAAGCATGAATATATTGAAGATGTTGGAGAATGTCACAACCAC ATGATTGGGATTGGGCCAGTCTTAAATATCTACCCATGGAATAAAGAGCTGTATTTGATC AGATTATTCAAAGAAAAACAAAACACGGTCAACAGGCAGTGGAACTCCTCACTCCAATGT GATATTCCTGAGAGAATATTGACTTATCGTCACTTCGTCTCTGGGACAAGTATTGAACAT AGGGGAAGCCTTATATAAAATTGTTCAATAAACAAAAGATGTCTTTAAAAAAAAAAAAAA AAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC025269 unedited NAAAGTTCAAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGATTCTGCCCACTCTG CGGAGTTAGCAGTCACGACCTCCAGCACAGGATGTGGTACCACAGATTGTCCCACCTACA CAGCAGGCTTCAGGACTTGCTGAAGGGAGGAGTCATATATCCGGCCCTTCCACAGCCCAA CTTCAAAAGCTTACTTCCTTTAGCTGTCCATTGGCACCATACAGCCTCCAAGTCTCTGAC TTGTGCTTGGCAGCAACATGAAGATCATTTTGAGCTGAAATATGCTAATACCGTGATGCG CTTTGATTACGTCTGGCTTCGAGACCACTGCCGCTCAGCATCGTGCTACAACTCTAAGAC TCACCAGCGCAGCCTGGATACTGCCAGTGTGGATTTATGTATCAAGCCAAAGACCATTCG TCTGGATGAGACCACACTCTTTTTCACTTGGCCAGATGGTCATGTGACTAAATATGATTT GAATTGGCTGGTGAAAAACAGCTATGAAGGGCAGAAACAAAAAGTCATCCAGCCTAGAAT ACTATGGAATGCTGAAATCTACCAGCAAGCCCAAGTTCCATCGGTAGATTGCCAGAGCTT CTTAGAAACCAACGAGGGACTGAAGAAGTTTCTGCAAAACTTTCTGCTCTATGGAATTGC ATTCGTAGAAAATGTCCCTCCCACTCAAGAGCACACAGAGAAGTTGGCAGANAGGATCAG CTTAATCAGAGAAACCATTTATGGGAGGATGTGGTATTTCACTTCAGACTTCTCCAGAGG TGACACTGCGTACACCAAGCTAGCTCTGGATCGGCACACTGACACTACCTATTTTTCAGA GCCCCTGTGCATTTCAGTGNTTCATTGTCTTAACATTGAAGACTGGNTGCAGGACACTGC TAGTAGATGAT
Restriction Sites	Please inquire
ACCN	BC025269
Insert Size	1264 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC025269.1, AAH25269.1
RefSeq Size	1264 bp
Locus ID	55217
Protein Pathways	Lysine degradation
Gene Summary	This gene encodes the protein trimethyllysine dioxygenase which is the first enzyme in the carnitine biosynthesis pathway. Carnitine play an essential role in the transport of activated fatty acids across the inner mitochondrial membrane. The encoded protein converts trimethyllysine into hydroxytrimethyllysine. A pseudogene of this gene is found on chromosome X. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

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