Protein Kinase D2 (PRKD2) (BC025307) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HSPC187; nPKC-D2; PKD2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC025307 edited TGAAGAGCTGAGCCGTAGCACCACCGAACTCCTGCCTCGCCGTCCCCCGTCATCCTCTTC CTCCTCTTCTGCCTCATCGTATACGGGCCGCCCCATTGAGCTGGACAAGATGCTGCTCTC CAAGGTCAAGGTGCCGCACACCTTCCTCATCCACAGCTATACACGGCCCACCGTTTGCCA GGCTTGCAAGAAACTCCTCAAGGGCCTCTTCCGGCAGGGCCTGCAATGCAAAGACTGCAA GTTTAACTGTCACAAACGCTGCGCCACCCGCGTCCCTAATGACTGCCTGGGGGAGGCCCT TATCAATGGAGATGTGCCGATTTCAGCGAGGCTGACAAGAGCGCCCTCATGGATGAGTCA GAGGACTCCGGTGTCATCCCTGGCTCCCACTCAGAGAATGCGCTCCACGCCAGTGAGGAG GAGGAAGGCGAGGGAGGCAAGGCCCAGAGCTCCCTGGGGTACATCCCCCTAATGAGGGTG GTGCAATCGGTGCGACACACGACGCGGAAATCCAGCACCACGCTGCGGGAGGGTTGGGTG GTTCATTACAGCAACAAGGACACGCTGAGAAAGCGGCACTATTGGCGCCTGGACTGCAAG TGTATCACGCTCTTCCAGAACAACACGACCAACAGATACTATAAGGAAATTCCGCTGTCA GAAATCCTCACGGTGGAGTCCGCCCAGAACTTCAGCCTTGTGCCGCCGGGCACCAACCCA CACTGCTTTGAGATCGTCACTGCCAATGCCACCTACTTCGTGGGCGAGATGCCTGGCGGG ACTCCGGGTGGGCCAAGTGGGCAGGGGGCTGAGGCCGCCCGGGGCTGGGAGACAGCCATC CGCCAGGCCCTGATGCCCGTCATCCTTCAGGACGCACCCAGCGCCCCAGGCCACGCGCCC CACAGACAAGCTTCTCTGAGCATCTCTGTGTCCAACAGTCAGATCCAAGAGAATGTGGAC ATTGCCACTGTCTACCAGATCTTCCCTGACGAAGTGCTGGGCTCAGGGCAGTTTGGAGTG GTCTATGGAGGAAAACACCGGAAGACAGGCCGGGACGTGGCAGTTAAGGTCATTGACAAA CTGCGCTTCCCTACCAAGCAGGAGAGCCAGCTCCGGAATGAAGTGGCCATTCTGCAGAGC CTGCGGCATCCCGGGATCGTGAACCTGGAGTGCATGTTCGAGACGCCTGAGAAAGTGTTT GTGGTGATGGAGAAGCTGCATGGGGACATGTTGGAGATGATCCTGTCCAGTGAGAAGGGC CGGCTGCCTGAGCGCCTCACCAAGTTCCTCATCACCCAGATCCTGGTGGCTTTGAGACAC CTTCACTTCAAGAACATTGTCCACTGTGACTTGAAACCAGAAAACGTGTTGCTGGCATCA GCAGACCCATTTCCTCAGGTGAAGCTGTGTGACTTTGGCTTTGCTCGCATCATCGGCGAG AAGTCGTTCCGCCGCTCAGTGGTGGGCACGCCGGCCTACCTGGCACCCGAGGTGCTGCTC AACCAGGGCTACAACCGCTCGCTGGACATGTGGTCAGTGGGCGTGATCATGTACGTCAGC CTCAGCGGCACCTTCCCTTTCAACGAGGATGAGGACATCAATGACCAGATCCAGAACGCC GCCTTCATGTACCCGGCCAGCCCCTGGAGCCACATCTCAGCTGGAGCCATTGACCTCATC AACAACCTGCTGCAGGTGAAGATGCGCAAACGCTACAGCGTGGACAAATCTCTCAGCCAC CCCTGGTTACAGGAGTACCAGACGTGGCTGGACCTCCGAGAGCTGGAGGGGAAGATGGGA GAGCGATACATCACGCATGAGAGTGACGACGCGCGCTGGGAGCAGTTTGCAGCAGAGCAT CCGCTGCCTGGGTCTGGGCTGCCCACGGACAGGGATCTCGGTGGGGCCTGTCCACCACAG GACCACGACATGCAGGGGCTGGCGGAGCGCATCAGTGTTCTCTGAGGTCCTGTGCCCTCG TCCAGCTGCTGCCCTCCACAGCGGTTCTTCACAGGATCCCAGCAATGAACTGTTCTAGGG AAAGTGGCTTCCTGCCCAAACTGGATGGGACACGTGGGGAGTGGGGTGGGGGGAGCTATT TCCAAGGCCCCTCCCTGTTTCCCCAGCAATTAAAACGGACTCATCTCTGGCCCCATGGCC TTGATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC025307 unedited TTTGGNNNCCGTACAACATTTGTNATACCACTCACTATAGGGCGGCCGCGAATTCGCACG GGTGAAGAGCTGAGCCGTAGCACCACCGAACTCCTGCCTCGCCGTCCCCCGTCATCCTCT TCCTCCTCTTCTGCCTCATCGTATACGGGCCGCCCCATTGAGCTGGACAAGATGCTGCTC TCCAAGGTCAAGGTGCCGCACACCTTCCTCATCCACAGCTATACACGGCCCACCGTTTGC CAGGCTTGCAAGAAACTCCTCAAGGGCCTCTTCCGGCAGGGCCTGCAATGCAAAGACTGC AAGTTTAACTGTCACAAACGCTGCGCCACCCGCGTCCCTAATGACTGCCTGGGGGAGGCC CTTATCAATGGAGATGTGCCGATTTCAGCGAGGCTGACAAGAGCGCCCTCATGGATGAGT CAGAGGACTCCGGTGTCATCCCTGGCTCCCACTCAGAGAATGCGCTCCACGCCAGTGAGG AGGAGGAAGGCGAGGGAGGCAAGGCCCAGAGCTCCCTGGGGTACATCCCCCTAATGAGGG TGGTGCAATCGGTGCGACACACGACGCGGAAATCCAGCACCACGCTGCGGGAGGGTTGGG TGGTTCATTACAGCAACAAGGACACGCTGAGAAAGCGGCACTATTGGCGCCTGGACTGCA AGTGTATCACGCTCTTCCAGAACAACACGACCAACAGATACTATAAGGAAATTCCGCTGT CAGAAATCCTCACGGTGGAGTCCGCCCAGAACTTCAGCCTTGTGCCGCCGGGCACCAACC CACACTGCTTTGAGATCGTCACTGCCNATGCCACCTACTTCGTGGGCGAGATGCCTGGCG GGACTCCGGGTGGGCCAGTGGGCAGGGG
Restriction Sites	Please inquire
ACCN	BC025307
Insert Size	2199 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC025307.1
RefSeq Size	2199 bp
Locus ID	25865
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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