CDK6 (BC027989) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MCPH12; PLSTIRE
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC027989 edited CCACGCGTCCGCTTTCTCTTAGGTTTCAGGAAGACTGTCACATACAGTAAGCTTTTTTCC TTCTGACTTATAATAGAAAATGTTTTGAAAGTAAAAAAAAAAAATCTAATTTGGAAATTT GACTTGTTAGTTTCTGTGTTTGAAATCATGGTTCTAGAAATGTAGAAATTGTGTATATCA GATACTCATCTAGGCTGTGTGAACCAGCCCAAGATGACCAACATCCCCACACCTCTACAT CTCTGTCCCCTGTATCTCTTCCTTTCTACCACTAAAGTGTTCCCTGCTACCATCCTGGCT TGTCCACATGGTGCTCTCCATCTTCCTCCACATCATGGACCACAGGTGTGCCTGTCTAGG CCTGGCCACCACTCCCAACTTGACCTAGCCACATTCATCTAGAGATGGTTCCTGATGCTG GGCACAGACTGTGCTCATGGCACCCATTAGAAATGCCTCTAGCATCTTTGTATGCATCTT GATTTTTAAACCAAGTCATTGTACAGAGCATTCAGTTTTGGCTGTGGTACCAAGAGAAAA ACTAATCAAGAATATAAACCACATTCCAGGCTGCTGTTTTCTCTCCATCTACAGGCCACA CTTTTACTGTATTTCTTCATACTTGAAATTCATTCTGCTATTTTCATATCAGGGTACAGA CTTATAAGGGTGCATGTTCCTTAAAGGTGCATAATTATTCTTATTCCGTTTGCTTATATT GCTACAGAATGCTCTGTTTTGGTGCTTTGAGTTCTGCAGACCCAAGAAGCAGTGTGGAAA TTCACTGCCTGGGACACAGTCTTATAAGAATGTTGGCAGGTGACTTTGTATCAGATGTTG CTTCTCTTTTCTCTGTACACAGATTGAGAGTTACCACAGTGGCCTGTCGGGTCCACCCTG TGGGTGCAGCACAGCTCTCTGAAAGCAAGAACCTTCCTACCTATTCTAACGTTTTTGCCC TCTAAGAAAAATGGCCTCAGGTATGGTATAGACATAGCAAGAGGGGAAGGGCTGTCTCAC TCTAGCAACCATCCCTCCATTACACACAGAAAGCCCTCTTGAAGCAAAAGAAGAAGAAAG AAAGAAAGCTTATCTCTAAGGCTACTGTCTTCAGAATGCTCTGAGCTGAATGCTCTTGCT CCTTTCCCAAGAGGCAGATGAAAATATAGCCAGTTTATCTATACCCTTCCTATCTGAGGA GGAGAATAGAAAAGTAGGGTAAATATGTAACGTAAAATATGTCATTCAAGGACCACCAAA ACTTTAAGTACCCTATCATTAAAAATCTGGTTTTAAAAGTAGCTCAAGTAAGGGATGCTT TGTGACCCAGGGTTTCTGAAGTCAGATAGCCATTCTTACCTGCCCCTTACTCTGACTTAT TGGGAAAGGGAGAACTGCAGTGGTGTTTCTGTTGCAGTGGCAAAGGTAACATGTCAGAAA ATTCAGAGGGTTGCATACCAATAATCCTTTGGAAACTGGATGTCTTACTGGGTGCTAGAA TGAAAATGTAGGTATTTATTGTCAGATGATGAAGTTCATTGTTTTTTTCAAAATTGGTGT TGAAATATCACTGTCCAATGTGTTCACTTATGTGAAAGCTAAATTGAATGAGGCAAAAAG AGCAAATAGTTTGTATATTTGTAATACCTTTTGTATTTCTTACAATAAAAATATTGGTAG CAAATAAAAATAATAAAAACAATAACTTTAAACTGCAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC027989 unedited GGGTTANGATTTGTATACGACTCACTATAGGGCGGCCGCGATTCCCGGGATATCGTCGAC CCACGCGTCCGCTTTCTCTTAGGTTTCAGGAAGACTGTCACATACAGTAAGCTTTTTTCC TTCTGACTTATAATAGAAAATGTTTTGAAAGTAAAAAAAAAAAATCTAATTTGGAAATTT GACTTGTTAGTTTCTGTGTTTGAAATCATGGTTCTAGAAATGTAGAAATTGTGTATATCA GATACTCATCTAGGCTGTGTGAACCAGCCCAAGATGACCAACATCCCCACACCTCTACAT CTCTGTCCCCTGTATCTCTTCCTTTCTACCACTAAAGTGTTCCCTGCTACCATCCTGGCT TGTCCACATGGTGCTCTCCATCTTCCTCCACATCATGGACCACAGGTGTGCCTGTCTAGG CCTGGCCACCACTCCCAACTTGACCTAGCCACATTCATCTAGAGATGGTTCCTGATGCTG GGCACAGACTGTGCTCATGGCACCCATTAGAAATGCCTCTAGCATCTTTGTATGCATCTT GATTTTTAAACCAAGTCATTGTACAGAGCATTCAGTTTTGGCTGTGGTACCAAGAGAAAA ACTAATCAAGAATATAAACCACATTCCAGGCTGCTGTTTTCTCTCCATCTACAGGCCACA CTTTTACTGTATTTCTTCATACTTGAAATTCATTCTGCTATTTTCATATCAGGGTACAGA CTTATAAGGGTGCATGTTCCTNTAAGGTGCATAATTATTCTTATTCCGTTTGCTTATATT GCTACAGAATGCTCTGTTTTGGTGCTTTGAGTTCTGCAGACCCAAGAAGCAGTGTGGGAA TTCACTGCCTGGGGACACAGTCTATAAGAATGTNGGCAGGTGACTTTGTATCAGATGTGG CTTCTCTTTTTCTGN
Restriction Sites	Please inquire
ACCN	BC027989
Insert Size	1748 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC027989.1
RefSeq Size	1748 bp
Locus ID	1021
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Small cell lung cancer
Gene Summary	The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly. [provided by RefSeq, Aug 2017]

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