KIAA0859 (METTL13) (BC029083) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	5630401D24Rik; CGI-01; feat; KIAA0859
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC029083 edited CACTGCAGTGTCCCGGAGCCTGCGTGTGGTGGGCAAGCTCCTCAAATGGTATCTCACAGG GAATAGGGGAGTCTTGAAAACGCAGCTTCGGCAGTAGGAACATGAACCTCTTACCTAAAA GTTCCAGGGAGTTTGGCTCCGTTGACTATTGGGAGAAGTTCTTCCAGCAGCGAGGAAAGA AAGCTTTCGAGTGGTATGGAACCTACCTGGAACTGTGCGGGGTGCTACATAAATATATCA AGCCCAGGGAAAAGGTGCTGGTGATTGGGTGTGGCAACTCAGAACTGAGTGAGCAACTGT ATGATGTGGGCTATCGGGATATAGTGAACATCGACATCAGTGAGGTTGTCATCAAGCAAA TGAAGGAATGTAATGCCACCCGACGGCCCCAGATGAGCTTCTTGAAGATGGACATGACGC AGATGGAGTTTCCTGATGCCTCGTTCCAGGTGGTGTTGGACAAGGGCACCCTGGATGCTG TCCTGACAGATGAGGAAGAGAAGACCTTACAACAGGTGGACAGGATGCTGGCTGAGGTTG GCCGTGTCCTGCAGGTGGGCGGTCGCTATCTCTGCATCTCCCTGGCTCAGGCTCACATCC TGAAGAAAGCAGTGGGCCACTTCTCCCGGGAGGGGTGGATGGTGAGGGTGCACCAAGTGG CCAACAGCCAGGACCAGGTGTTGGAAGCAGAGCCTCAGTTCTCCTTGCCTGTCTTTGCCT TCATCATGACCAAGTTCAGGCCAGTCCCTGGCTCTGCCCTTCAGATCTTTGAGCTGTGTG CTCAGGAGCAGCGCAAGCCTGTGCGGCTGGAGAGTGCCGAGCGGCTGGCCGAGGCGGTGC AGGAGCGACAGCAGTATGCCTGGCTGTGCAGCCAGCTGCGCCGCAAGGCCAGGCTGGGGA GTGTGTCTCTGGACTTGTGCGATGGGGACACGGGGGAGCCACGCTACACCCTCCACGTGG TGGACAGCCCCACTGTGAAACCATCGCGGGACAATCATTTTGCGATTTTCATCATCCCTC AGGGCCGGGAGACCGAGTGGCTCTTTGGCATGGATGAGGGCCGGAAACAGCTGGCGGCCA GTGCTGGCTTCAGGAGGTTGATTACAGTGGCCCTTCACCGAGGTCAGCAGTATGAAAGCA TGGACCACATCCAAGCTGAGCTGTCGGCTAGAGTCATGGAGCTGGCCCCAGCTGGGATGC CCACCCAGCAGCAGGTCCCCTTTCTGTCTGTGGGTGGGGACATTGGGGTCCGGACCGTTC AGCACCAAGACTGCAGCCCCTTGAGCGGTGACTATGTCATTGAGGATGTGCAAGGGGATG ACAAGCGATACTTCCGTCGACTGATCTTCCTCAGCAACAGGAATGTGGTGCAGTCCGAAG CCAGGTTGCTGAAGGATGTGTCTCACAAAGCCCAGAAGAAGCGGAAAAAGGACAGGAAGA AGCAGCGGCCTGCTGATGCGGAGGACCTCCCTGCAGCCCCGGGGCAGTCCATTGATAAGA GTTACCTGTGTTGTGAACACCACAAAGCCATGATCGCTGGCCTTGCCCTGCTGAGAAACC CAGAGCTACTCCTAGAGATCCCACTGGCATTGTTGGTGGTAGGCCTGGGCGGGGGCAGCC TCCCCCTCTTTGTCCACGATCATTTTCCAAAGTCCTGCATTGATGCTGTGGAGATCGATC CCTCCATGTTGGAAGTGGCCACCCAGTGGTTTGGCTTCTCCCAGAGTGACCGAATGAAGG TCCACATTGCAGATGGCCTGGACTATATCGCCAGCTTGGCAGGAGGAGGAGAAGCACGGC CTTGCTACGATGTCATAATGTTTGATGTTGACAGTAAGGACCCAACACTGGGAATGAGTT GTCCGCCCCCAGCATTTGTGGAGCAATCTTTTCTACAGAAGGTTAAAAGCATCTTGACTC CTGAAGGTGTTTTTATTCTCAACCTTGTGTGCCGAGACTTGGGGCTAAAAGACTCAGTGC TGGCTGGGCTCAAGGCAGTGTTCCCCCTCCTATATGTCCGGCGAATTGAGGGTGAAGTGA ATGAGATCCTGTTCTGTCAGCTGCACCCTGAGCAAAAACTTGCCACACCAGAGCTCCTAG AAACAGCCCAGGCTTTGGAGCGGACCCTGAGGAAGCCTGGGAGGGGTTGGGATGACACGT ATGTCTTGTCAGATATGCTCAAGACGGTGAAAATTGTGTGACTGCTTAGGCCAAGCAGCC CTCCTGCCTAGACTGACCTTGGACTCCCAGCCTGCCAGAGAATGAAGAAATACAACGCCC AGTACTTTTGAAGCTTCGTATTTTTCTTGGTTTCACACTCAGCTACATGTGACCTCCAGC TTGGTGAGGTTGCCTGAAGATTAGGGAAAATAAAAATGTCCTTCCCAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC029083 unedited NGGTTTTGATTTGTATACGACTCTTATAGGCGGCCGCGNATTCCCGGGATATCGTCGACC CACGCGTCCGCACTGCAGTGTCCCGGAGCCTGCGTGTGGTGGGCAAGCTCCTCAAATGGT ATCTCACAGGGAATAGGGGAGTCTTGAAAACGCAGCTTCGGCAGTAGGAACATGAACCTC TTACCTAAAAGTTCCAGGGAGTTTGGCTCCGTTGACTATTGGGAGAAGTTCTTCCAGCAG CGAGGAAAGAAAGCTTTCGAGTGGTATGGAACCTACCTGGAACTGTGCGGGGTGCTACAT AAATATATCAAGCCCAGGGAAAAGGTGCTGGTGATTGGGTGTGGCAACTCAGAACTGAGT GAGCAACTGTATGATGTGGGCTATCGGGATATAGTGAACATCGACATCAGTGAGGTTGTC ATCAAGCAAATGAAGGAATGTAATGCCACCCGACGGCCCCAGATGAGCTTCTTGAAGATG GACATGACGCAGATGGAGTTTCCTGATGCCTCGTTCCAGGTGGTGTTGGACAAGGGCACC CTGGATGCTGTCCTGACAGATGAGGAAGAGAAGACCTTACAACAGGTGGACAGGATGCTG GCTGAGGTTGGCCGTGTCCTGCAGGTGGGCGGTCGCTATCTCTGCATCTCCCTGGCTCAG GCTCACATCCTGAAGAAAGCAGTGGGCCACTTCTCCCGGGAGGGGTGGATGGGTGAGGGT GCACCAAGTGGCCAACAGCCAGGACCAGGTGTTGGAAGCAGAGCCTCAGTTCTCCTTGCC TGTCTTTGCCTTCATCATGACCAAGTTCANGCCAGTCCCTGGCTCTGCCCTTCAGATCTT TGAGCTGTGTGCTCAGGAGCAGCGCAAGCCTGTGCGGCTGGAGAGTGCCTAGCGGCTGGC CCAGGCGGTGCANGAA
Restriction Sites	Please inquire
ACCN	BC029083
Insert Size	2420 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC029083.1, AAH29083.1
RefSeq Size	2420 bp
Locus ID	51603
Protein Families	Druggable Genome
Gene Summary	Dual methyltransferase that catalyzes methylation of elongation factor 1-alpha (EEF1A1 and EEF1A2) at two different positions, and is therefore involved in the regulation of mRNA translation (PubMed:30612740, PubMed:30143613). Via its C-terminus, methylates EEF1A1 and EEF1A2 at the N-terminal residue 'Gly-2' (PubMed:30143613). Via its N-terminus dimethylates EEF1A1 and EEF1A2 at residue 'Lys-55' (PubMed:30612740, PubMed:30143613). Has no activity towards core histones H2A, H2B, H3 and H4 (PubMed:30612740).[UniProtKB/Swiss-Prot Function]

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