FRMD8 (BC033851) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FERM domain containing 8; FKSG44; FLJ32216; FLJ90369; MGC31785
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC033851 edited GTCAGCTGCGTCCTTAGCGGGAGCCCGAGTGCGGGCGGTGGCGGGCTTGGCGGCGGGGCA GGATTCCAGGCAGGAGCCTTGCCTCTCAGGTGGCGGGCTCTGCGACCCTGCGAGATGGAC GGGACAGAAGGCAGTGCCGGGCAGCCCGGCCCCGCTGAGCGATCCCACCGAAGCAGCGTG TCCTCCGTGGGAGCCCGAGGTGGGTCCCACCCGCATCTCCTCTTCCACACCCTCCTGGGA CCCCAGCCCTGGAGAAGTAGCTCTGGCTTGTCTTCTTTTTCATTAAAGATAATTTATGTA TCGACTAAGAAATAATAAGGTACATTCAGATGTGGAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC033851 unedited CATTCTTGGAGTTTTGTNAATACCCACTTCCTGTNAGGGNCGGACCGCGAAATTCCCGGG ATGTCAGCTGCGTCCTTAGCGGGAGCCCGAGTGCGGGCGGTGGCGGGCTTGGCGGCGGGG CAGGATTCCAGGCAGGAGCCTTGCCTCTCAGGTGGCGGGCTCTGCGACCCTGCGAGATGG ACGGGACAGAAGGCAGTGCCGGGCAGCCCGGCCCCGCTGAGCGATCCCACCGAAGCAGCG TGTCCTCCGTGGGAGCCCGAGGTGGGTCCCACCCGCATCTCCTCTTCCACACCCTCCTGG GACCCCAGCCCTGGAGAAGTAGCTCTGGCTTGTCTTCTTTTTCATTAAAGATAATTTATG TATCGACTAAGAAATAATAAGGTACATTCAGATGTGGAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAGGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGG GTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGT GCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTT CTATAATATTATGGGGTGGAGGGGGGTGAGTATGGGAGCAAGGGGCAAGTTGGGAAGACA ACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGG CTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTG TTGGGATTTCAGGCATGCTTGACCAGGCTCAACTAATTTTGGTTTTTTGGTAGAAACCGG GTTTCACATTTTGGCCAGGCTGGTCTCCACTCCTAATCTCAGGTGATCTACCACCTTGGG CTCCAAATTGCTGGATTTCAGGGTGAAACACTGCTCCCTTCCTGTCCTTTGAT
Restriction Sites	Please inquire
ACCN	BC033851
Insert Size	377 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC033851.1, AAH33851.1
RefSeq Size	377 bp
Locus ID	83786
Gene Summary	Promotes the cell surface stability of iRhom1/RHBDF1 and iRhom2/RHBDF2 and prevents their degradation via the endolysosomal pathway. By acting on iRhoms, involved in ADAM17-mediated shedding of TNF, amphiregulin/AREG, HBEGF and TGFA from the cell surface (PubMed:29897333, PubMed:29897336). Negatively regulates Wnt signaling, possibly by antagonizing the recruitment of AXIN1 to LRP6 (PubMed:19572019).[UniProtKB/Swiss-Prot Function]

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