BCAT1 (BC033864) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BCATC; BCT1; ECA39; MECA39; PNAS121; PP18
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC033864 edited GGTGGATGCTGCGGCATCGGAGGACCCTGCTGGTGGAGGAAATGGTTCACGCCCGTCCCC GTTCCCTTTGCAGGCTTGCTATTGTGCGTCTGTGATTGACAAGACCACGAGGCTGAGCGC GCCCTGGAGATTTTTCTATAAATGGCTTAACACCCCAGTCTAGACTATTTGCTCGGATAT AAGGGAGACAATTGTTTTTTTGTTCTTTGCCGGCGAACCCTGGCTCTGTAGGGCTGACCT GGAATTTAACCAGTCTTCCCTGAGCCGGCGGAGGAGGACAAAAACCGCCGCGACCCCGGC AGGGTGGGAAGTGCAGGGCAGCGCTCCCAAGACACGCTTGTTGGAGGTTCGGGCCTGGGT GCTTGGTTGTCTGAGCCTCCTTTTTTGTGTTTGCCTGGGTCCTGGAGAGGAGCGCACGGT ATCATGGATTGCAGTAACGGATGCTCCGCAGAGTGTACCGGAGAAGGAGGATCAAAAGAG GTGGTGGGGACTTTTAAGGCTAAAGACCTAATAGTCACACCAGCTACCATTTTAAAGGAA AAACCAGACCCCAATAATCTGGTTTTTGGAACTGTGTTCACGGATCATATGCTGACGGTG GAGTGGTCCTCAGAGTTTGGATGGGAGAAACCTCATATCAAGCCTCTTCAGAACCTGTCA TTGCACCCTGGCTCATCAGCTTTGCACTATGCAGTGGAATTATTTGAAGGATTGAAGGCA TTTCGAGGAGTAGATAATAAAATTCGACTGTTTCAGCCAAACCTCAACATGGATAGAATG TATCGCTCTGCTGTGAGGGCAACTCTGCCGGTATTTGACAAAGAAGAGCTCTTAGAGTGT ATTCAACAGCTTGTGAAATTGGATCAAGAATGGGTCCCATATTCAACATCTGCTAGTCTG TATATTCGTCCTACATTCATTGGAACTGAGCCTTCTCTTGGAGTCAAGAAGCCTACCAAA GCCCTGCTCTTTGTACTCTTGAGCCCAGTGGGACCTTATTTTTCAAGTGGAACCTTTAAT CCAGTGTCCCTGTGGGCCAATCCCAAGTATGTAAGAGCCTGGAAAGGTGGAACTGGGGAC TGCAAGATGGGAGGGAATTACGGCTCATCTCTTTTTGCCCAATGTGAAGCAGTAGATAAT GGGTGTCAGCAGGTCCTGTGGCTCTATGGAGAGGACCATCAGATCACTGAAGTGGGAACT ATGAATCTTTTTCTTTACTGGATAAATGAAGATGGAGAAGAAGAACTGGCAACTCCTCCA CTAGATGGCATCATTCTTCCAGGAGTGACAAGGCGGTGCATTCTGGACCTGGCACATCAG TGGGACACAGAACTCAGCTTGTTTTCAATTAATTTGCCTGATTTTCTGCAGTTCATTTAC TTTTGAACAACATAATTGCAATTGTAGACTGAGAGAAATTGAAACTTTCAAAGAGCCATA TTTCTATTGCAGATATATTTTCCTGCTCTTCCAAATCTACTTACAGCATGAGTTCTTCTT TTAAATATTCAAATATTTTGAATATTGCCAAGAGCTTTGATTTCCATTTTTATCTCTTGT GGGTTTATAAATTAAGAAAAAATACTCATCTTATTTTTTTAAACCTCTCTATTTTTATTG CCCTTTATTCAAATAACTTGTTGACAAACTTTGAACTTGAACCACTGAGGTAAAAGAACA AGAATTAAACAGATAGTTTAAACACATAGCTTAAAAGGATCTTTTTCCCATTTCCTATCC TTGAGCAAAGAATATATTCAAACACTTTGGCAGAAGTCAATGAGGTTATACCACTAATTC CATGATGAAAATCAACTGAATGTGATACTGAAAGAGAAGGAAGAGAATTGTCACTGTAAA GTCAACTGTTAGTCATATTAGGAAAAAAAATACATACAATACAATTTCTCAAATAAAGTC CAAATATACATTCAATGTTTAAAAATAATGAGTATTTCAGATATTTGAACTCAGTCTGTT CTTTATTCCATAAAAGATATAGGTAAGCCGTGCACGGTGGCTCACAACTATAATCCCAGC ACTTTGGCACTTTGGGAGGCTGAGGTGGGAGGATCACATGAGCCCAGCCTGGGCAACATA GGGAGACCGCTATCTTTACAAAATAAAATATAAAATATAAAACCTAGTTGGGCATGGCAG CATACACCTGTAGTCCCAGGTGCTCGGGAGACTGAGACAGGAGGATCGCTTGGGCCTGGG AGGTCGAGGCTGCAGTGAGCCAAGATTATGCCACTGCATTCCAGCCTGGGTGACAGGGCA AGACCCTGTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC033864 unedited CCGTTCCGCATAGNAAACNATCATATAGGCGGCCGCCATTCCCGGGAGGTGGATGCTGCG GCATCGGAAGACCCTGCTGGTGGAGGAAATGGTTCACGCCCGTCCCCGTTCCCTTTGCAG GCTTGCTATTGTGCGTCTGTGATTGACAAGACCACGAGGCTGAGCGCGCCCTGGAGATTT TTCTATAAATGGCTTAACACCCCAGTCTAGACTATTTGCTCGGATATAAAGGAGACAATT GTTTTTTTGTTCTTTGCCGGCGAACCCTGGCTCTGTAGGGCTGACCTGGAATTTAACCAG TCTTCCCTGAGCCGGCGGAGGAGGACAAAAACCGCCGCGACCCCGGCAGGGTGGGAAGTG CAGGGCAGCGCTCCCAAGACACCGCTTGTTGGAAGTTCGGGCCTGAGTGCTTGGTTGTCT GAGCCTCCTTTTTTGTGTTTGCCTGGGTCCTGGAGAGGAGCGCACGGTATCATGGATTGC AGTAACGGATGCTCCGCAGAGTGTACCGGATAAGGAGGATCAAAAGAAGTGGTGGGGACT TTTAAAGGCTAAAAACCTAATATTCACACCAGCTACCATTTTAAAGAAAAACCAGACCCC ATAATCTGGTTTTTGGAACTGGGTTCACGGATCATATGCTGACGGTGGATTGGGTCTCAG AGTTTGGATGGGAGAAACCCTATATCAAGCCTTTTTAGAAACTTGGTCTTGCACCCTGGC TTATAAGCTTTTGCACCATGCATGGGAATTCTTTGAAGATTGGAAGGCATTTCCAAGAGT AAATAATTAAAATTCGCTGGTTTAGNCCTAACCTCAACATGGAAAAAAAGGATTCATCCG GTGGGAAGGGAAATTTGCCGGGATTTTGAAAAAAAAGAACCCTTTAAGTGTATTTAAAAA CTTGGGAAAATTGGTN
Restriction Sites	Please inquire
ACCN	BC033864
Insert Size	2321 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC033864.1
RefSeq Size	2321 bp
Locus ID	586
Protein Families	Druggable Genome
Protein Pathways	Metabolic pathways, Pantothenate and CoA biosynthesis, Valine, leucine and isoleucine biosynthesis, Valine, leucine and isoleucine degradation
Gene Summary	This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]

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