EPB41 (BC039079) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	4.1R; EL1; erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked); erythrocyte surface protein band 4.1; HE; OTTHUMP00000003772; OTTHUMP00000003773; OTTHUMP00000003774
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC039079 edited CGGGAGGGCGCGCGCCAGGGTCGCTCGCTCGCTCCCTCCCTCCGCTGGTGCGTTTAGTCA GTCAGCCAGCAGCAGCCGGCGGGCCCGCGAGCCGCAGAGGGCCCGAGCCTCGGACGCCGG CGCCTCCTCCTGCCATTGTTCGTCGGGCTGCAGCAGTGGCGGGCGCAGGAGCCCGGCCCC GGAGCCACCGCAACATCATGACAACAGAGAAGAGTTTAGTGACTGAGGCCGAAAATTCAC AGCACCAACAGAAGGAAGAGGGTGAGGAAGCCATAAACTCAGGCCAACAAGAACCTCAGC AGGAGGAATCTTGTCAAACAGCAGCTGAAGGAGATAATTGGTGTGAACAGAAGCTGAAAG CTTCTAATGGAGACACTCCTACACATGAAGACTTGACCAAGAACAAGGAGCGGACATCAG AAAGCAGAGGACTTTCACGACTATTCTCCTCGTTTCTCAAAAGGCCCAAATCTCAGGTGT CCGAGGAAGAAGGCAAAGAAGTAGAGTCAGATAAAGAAAAAGGTGAAGGAGGTCAGAAAG AGATAGAATTTGGAACCAGTCTTGATGAAGAGATCATTTTAAAGGCCCCAATTGCAGCTC CTGAACCGGAACTCAAAACAGACCCATCTTTGGATCTTCATTCATTAAGCAGTGCAGAAA CACAGCCTGCTCAGGAAGAACTCAGAGAAGATCCAGATTTTGAAATTAAGGAAGGAGAAG GACTTGAAGAGTGCTCCAAAATAGAAGTAAAAGAAGAAAGCCCTCAATCAAAAGCAGAAA CAGAATTAAAAGCTTCCCAAAAACCAATCAGAAAACACAGGAACATGCACTGCAAGGTTT CTTTGTTGGATGACACAGTTTATGAATGTGTTGTGGAGAAACATGCTAAGGGACAAGATT TGCTTAAACGAGTATGTGAGCATCTCAATCTTTTGGAAGAAGACTATTTTGGTCTAGCCA TTTGGGATAACGCAACCTCTAAGACATGGCTGGATTCCGCCAAAGAAATAAAAAAGCAGG TTCGTGGTGTCCCTTGGAATTTTACATTTAATGTAAAGTTTTATCCACCTGACCCAGCAC AGTTAACAGAAGACATAACAAGATATTATTTATGTCTTCAGCTTCGGCAGGACATAGTTG CAGGACGTCTGCCCTGTTCCTTTGCAACCTTAGCATTATTAGGTTCTTACACCATCCAGT CTGAACTGGGAGACTACGACCCAGAACTCCATGGCGTGGATTATGTTAGTGATTTTAAAC TGGCCCCGAATCAGACCAAGGAACTTGAAGAGAAGGTCATGGAACTGCATAAGTCATACA GGTCCATGACTCCAGCTCAGGCTGACTTGGAGTTTCTTGAGAATGCCAAAAAGTTGTCTA TGTATGGAGTTGATCTTCATAAAGCAAAGGACTTGGAAGGAGTAGATATCATCCTAGGTG TCTGCTCTAGTGGCCTTCTGGTTTACAAAGATAAGCTGAGAATTAACCGCTTCCCTTGGC CCAAAGTGCTGAAGATTTCTTATAAACGTAGTAGCTTTTTCATCAAGATTCGGCCTGGAG AGCAAGAGCAGTATGAAAGTACCATCGGATTCAAACTTCCCAGTTACCGAGCAGCTAAGA AATTATGGAAAGTCTGTGTAGAACATCACACGTTTTTCAGATTGACATCTACAGACACCA TTCCCAAAAGCAAATTTCTTGCGCTAGGATCCAAATTTCGATACAGTGGCCGGACTCAAG CTCAGACCAGGCAAGCTAGTGCTCTAATTGACAGGCCTGCCCCACACTTCGAGCGTACAG CAAGTAAACGGGCGTCCCGGAGCCTCGATGGAGCAGCAGCTGTCGATTCGGCAGACCGAA GTCCTCGGCCCACTTCTGCACCTGCCATTACTCAGGGTCAGGTTGCAGAAGGTGGCGTCC TAGATGCCTCTGCTAAAAAAACAGTGGTCCCTAAAGCACAGAAGGAAACAGTGAAGGCTG AAGTGAAAAAGGAAGACGAGCCACCTGAGCAAGCTGAGCCAGAGCCCACAGAAGCATGGA AGGTGGAAAAAACCCACATCGAGGTGACAGTACCCACCTCAAATGGTGACCAAACACAGA AAAAGAGAGAAAGACTAGATGGTGAAAACATTTATATCAGACATAGCAATTTAATGTTGG AGGATTTAGACAAGAGTCAAGAGGAGATCAAAAAACATCATGCCAGCATCAGTGAGCTGA AAAAGAACTTCATGGAGTCTGTACCAGAACCACGGCCTAGTGAATGGGATAAACGCTTAT CCACTCACTCACCCTTCCGAACTCTTAACATCAATGGGCAAATCCCCACAGGAGAAGGAG TGAGTACTTTGTCCACATGACCAATTGTGAAAATGGAGGGAATAAATGTTTTTATGTATT AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC039079 unedited ACTCTGATTTGTAAACGACTCCTTATAGGCGGCCGACGATTCCCGGGATCGGGAGGGCGC GCGCCAGGGTCGCTCGCTCGCTCCCTCCCTCCGCTGGTGCGTTTAGTCAGTCAGCCAGCA GCAGCCGGCGGGCCCGCGAGCCGCAGAGGGCCCGAGCCTCGGACGCCGGCGCCTCCTCCT GCCATTGTTCGTCGGGCTGCAGCAGTGGCGGGCGCAGGAGCCCGGCCCCGGAGCCACCGC AACATCATGACAACAGAGAAGAGTTTAGTGACTGAGGCCGAAAATTCACAGCACCAACAG AAGGAAGAGGGTGAGGAAGCCATAAACTCAGGCCAACAAGAACCTCAGCAGGAGGAATCT TGTCAAACAGCAGCTGAAGGAGATAATTGGTGTGAACAGAAGCTGAAAGCTTCTAATGGA GACACTCCTACACATGAAGACTTGACCAAGAACAAGGAGCGGACATCAGAAAGCAGAGGA CTTTCACGACTATTCTCCTCGTTTCTCAAAAGGCCCAAATCTCAGGTGTCCGAGGAAGAA GGCAAAGAAGTAGAGTCAGATAAAGAAAAAGGTGAAGGAGGTCAGATAGAGATAGAATTT GGAACCAGTCTTGATGAAGAGATCATTTTAAAGGCCCCAATTGCAGCTCCTGAACCGGAA CTCANAACAGACCCATCTTTGGATCTTCATTCATTAAGCAGTGCAGAAACACAGCCTGCT CAGGAAGAACTCAGAGAAGATCCAGATTTTGAATTAAGGNAGGAGAANNGACTGAAGAGT GCTCCCAAATAGAAGTAAAAGAAAGAAGCCCCCTCATCAAAGCAGAACAAGATGAAAGCT TCCNNAAAACCATCAGAAACACAGGACATGCACTGCAAGGGTTCTTGTAGATGACACAGT TATGATGTGTTGTGAGAAA
Restriction Sites	Please inquire
ACCN	BC039079
Insert Size	2486 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC039079.1, AAH39079.1
RefSeq Size	2486 bp
Locus ID	2035
Protein Families	Druggable Genome
Protein Pathways	Tight junction
Gene Summary	The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. This complex plays a critical role in erythrocyte shape and deformability. Mutations in this gene are associated with type 1 elliptocytosis (EL1). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Oct 2009]

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