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NEDD4 (NM_198400) Human Untagged Clone
CAT＃: SC123396
NEDD4 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2
CNY 8,688.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NEDD4-1; RPF1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_198400 edited
ATGGCACAAAGCTTACGATTGCACTTTGCAGCCAGAAGAAGCAATACTTACCCTTTGTCA GAAACCTCCGGAGATGACTTGGATAGCCATGTTCACATGTGCTTCAAAAGACCAACACGG ATTTCAACGTCTAACGTTGTTCAAATGAAGCTGACTCCCAGACAGACTGCACTAGCTCCG TTAATAAAGGAAAACGTTCAGTCTCAAGAAAGATCATCTGTTCCCTCATCTGAAAATGTT AATAAAAAGAGCAGCTGTCTACAGATTTCACTACAGCCAACAAGGTACAGTGGATATCTT CAGTCTAGCAATGTCTTAGCTGATAGTGATGATGCTTCGTTTACTTGTATCTTGAAGGAT GGTATTTACAGTAGTGCTGTGGTCGATAATGAATTGAATGCTGTGAATGATGGTCACCTT GTAAGCAGTCCAGCCATTTGTAGTGGTAGCCTTAGTAACTTTTCAACCAGTGATAATGGG TCTTACAGCAGCAACGGTAGTGATTTTGGGTCATGTGCAAGTATCACAAGTGGAGGTTCA TATACTAACAGTGTCATCAGTGACAGTAGTAGTTATACTTTTCCACCAAGTGATGATACT TTTTTGGGTGGAAACTTACCTTCTGACAGCACCTCCAATAGAAGTGTGCCAAACAGGAAT ACTACTCCTTGTGAAATTTTTTCAAGAAGTACAAGTACAGATCCTTTTGTCCAGGATGAC TTGGAACATGGATTAGAGATTATGAAATTGCCAGTGAGCAGGAACACAAAAATTCCACTA AAACGTTACTCCTCCTTAGTCATTTTTCCTAGGAGTCCTTCAACTACCCGACCGACTTCT CCAACAAGTCTGTGTACTCTTCTGAGCAAAGGATCCTATCAAACTTCACACCAGTTTATT ATTTCTCCTAGTGAAATTGCACATAATGAGGATGGCACTAGTGCTAAAGGATTTCTTTCA ACAGCTGTCAATGGACTTCGGTTATCTAAAACAATTTGTACCCCCGGAGAAGTAAGAGAC ATACGGCCGCTTCACAGGAAGGGCTCGTTACAGAAGAAAATTGTTCTTTCGAATAATACT CCCAGACAGACTGTCTGTGAAAAGTCATCTGAAGGATATTCTTGTGTTTCAGTGCATTTC ACCCAACGAAAAGCAGCTACATTAGACTGTGAAACAACAAATGGTGATTGTAAACCAGAA ATGTCAGAAATTAAGCTTAATTCTGATTCAGAGTATATTAAGCTCATGCATAGGACATCT GCATGTTTGCCATCCTCCCAAAATGTAGATTGTCAAATAAATATCAATGGAGAATTGGAA AGACCACATTCACAGATGAACAAAAACCATGGTATTTTACGAAGAAGTATTTCATTGGGA GGAGCTTATCCAAATATTTCCTGTCTATCCAGCCTTAAGCACAATTGTTCTAAAGGGGGA CCATCTCAGTTACTCATAAAGTTTGCATCTGGAAATGAAGGTAAAGTGGATAATTTATCA AGAGACAGCAACAGAGATTGCACAAATGAACTGTCTAATTCTTGCAAGCCTGGCTGGGTT GTTTTGGACCAACCAGATGCTGCTTGCCATTTGCAGCAACAACAAGAACCTTCTCCTCTA CCTCCAGGGTGGGAAGAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAACCATGAA TCTAGAAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTGAGAAT GGCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGGCAGATATCCGAGGAA ACAGAAAGTGTTGACAACCGAGAGTCTTCCGAGAACTGGGAAATTATAAGAGAAGATGAA GCCACCATGTATAGCAACCAGGCCTTCCCATCACCTCCACCGTCAAGTAACTTGGATGTT CCAACTCATCTTGCAGAAGAATTGAATGCCAGACTCACCATTTTTGGAAATTCAGCCGTG AGCCAGCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAGCTTACAAGCCTATACT TTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCTGGATTACCACCAGGT TGGGAAGAAAAACAAGATGAAAGAGGAAGATCATATTATGTAGATCACAATTCCAGAACG ACTACTTGGACAAAGCCCACTGTACAGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGC CAGAGTTCTGCAGGCCCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACC CAGCCATCTGAAATTGAGCAAGGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATGCACCA AATGGGAGGCCTTTCTTTATTGACCACAACACTAAAACCACCACCTGGGAAGATCCAAGA TTGAAAATTCCAGCCCATCTGAGAGGAAAGACATCACTTGATACTTCCAATGATCTAGGG CCTTTACCTCCAGGATGGGAAGAGAGAACTCACACAGATGGAAGAATCTTCTACATAAAT CACAATATAAAAAGAACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGA CCAGCAGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAAAGTTG AAGAAGCAGAATGACATTCCAAACAAATTTGAAATGAAACTTCGCCGAGCAACTGTTCTT GAAGACTCTTACCGGAGAATTATGGGTGTCAAGAGAGCAGACTTCCTGAAGGCTCGACTG TGGATTGAGTTTGATGGTGAAAAGGGATTGGATTATGGAGGAGTTGCCAGAGAATGGTTC TTCCTGATCTCAAAGGAAATGTTTAACCCTTATTATGGGTTGTTTGAATATTCTGCTACG GACAATTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCACCTCTCT TACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCATGGCAAACTGTTGGAT GGTTTTTTCATCCGCCCATTTTACAAGATGATGCTTCACAAACCAATAACCCTTCATGAT ATGGAATCTGTGGATAGTGAATATTACAATTCCCTAAGATGGATTCTTGAAAATGACCCA ACAGAATTGGACCTCAGGTTTATCATAGATGAAGAACTTTTTGGACAGACACATCAACAT GAGCTGAAAAATGGTGGATCAGAAATAGTTGTCACCAATAAGAACAAAAAGGAATATATT TATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCAAATGGCTGCTTTTAAA GAGGGATTCTTTGAACTAATACCACAGGATCTCATCAAAATTTTTGATGAAAATGAACTA GAGCTTCTTATGTGTGGACTGGGAGATGTTGATGTGAATGACTGGAGGGAACATACAAAG TATAAAAATGGCTACAGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTA ATGATGGATTCAGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTCGGGTG CCTATGAATGGATTTGCTGAACTATACGGTTCAAATGGACCACAGTCATTTACAGTTGAA CAGTGGGGTACTCCTGAAAAGCTGCCAAGAGCTCATACCTGTTTTAATCGCCTGGACTTG CCACCTTATGAATCATTTGAAGAATTATGGGATAAACTTCAGATGGCAATTGAAAACACC CAGGGCTTTGATGGAGTTGATTAG |
| Restriction Sites | Please inquire |
| ACCN | NM_198400 |
| Insert Size | 5500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The ORF was fully sequenced and matches to NM_198400.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_198400.1, NP_940682.1 |
| RefSeq Size | 7084 bp |
| RefSeq ORF | 3744 bp |
| Locus ID | 4734 |
| UniProt ID | P46934 |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis, Ubiquitin mediated proteolysis |
| Gene Summary | This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (2) encodes isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212738 | NEDD4 (Myc-DDK-tagged)-Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2 |
CNY 8,680.00 |
|
| RC212738L1 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, Myc-DDK-tagged |
CNY 11,080.00 |
|
| RC212738L2 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, mGFP tagged |
CNY 14,350.00 |
|
| RC212738L3 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, Myc-DDK-tagged |
CNY 11,080.00 |
|
| RC212738L4 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, mGFP tagged |
CNY 14,350.00 |
|
| RG212738 | NEDD4 (tGFP-tagged) - Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2 |
CNY 13,680.00 |
