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NNT1 (CLCF1) (NM_013246) Human Untagged Clone
CAT＃: SC122789
CLCF1 (untagged)-Human cardiotrophin-like cytokine factor 1 (CLCF1), transcript variant 1
CNY 2,400.00
CNY 2,950.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BSF-3; BSF3; CISS2; CLC; NNT-1; NNT1; NR6 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_013246 edited
GCCGCGGCTCGCCCTCCCACTCCGCCAGCCTCCGGGAGAGGAGCCGCACCCGGCCGGCCC GGCCCCAGCCCCATGGACCTCCGAGCAGGGGACTCGTGGGGGATGTTAGCGTGCCTGTGC ACGGTGCTCTGGCACCTCCCTGCAGTGCCAGCTCTCAATCGCACAGGGGACCCAGGGCCT GGCCCCTCCATCCAGAAAACCTATGACCTCACCCGCTACCTGGAGCACCAACTCCGCAGC TTGGCTGGGACCTATCTGAACTACCTGGGCCCCCCTTTCAACGAGCCAGACTTCAACCCT CCCCGCCTGGGGGCAGAGACTCTGCCCAGGGCCACTGTTGACTTGGAGGTGTGGCGAAGC CTCAATGACAAACTGCGGCTGACCCAGAACTACGAGGCCTACAGCCACCTTCTGTGTTAC TTGCGTGGCCTCAACCGTCAGGCTGCCACTGCTGAGCTGCGCCGCAGCCTGGCCCACTTC TGCACCAGCCTCCAGGGCCTGCTGGGCAGCATTGCGGGCGTCATGGCAGCTCTGGGCTAC CCACTGCCCCAGCCGCTGCCTGGGACTGAACCCACTTGGACTCCTGGCCCTGCCCACAGT GACTTCCTCCAGAAGATGGACGACTTCTGGCTGCTGAAGGAGCTGCAGACCTGGCTGTGG CGCTCGGCCAAGGACTTCAACCGGCTCAAGAAGAAGATGCAGCCTCCAGCAGCTGCAGTC ACCCTGCACCTGGGGGCTCATGGCTTCTGACTTCTGACCTTCTCCTCTTCGCTCCCCCTT CAAACCCTGCTCCCACTTTGTGAGAGCCAGCCCTGTATGCCAACACCTGTTGAGCCAGGA GACAGAAGCTGTGAGCCTCTGGCCCTTTCCTGGACCGGCTGGGCGTGTGATGCGATCAGC CCTGTCTCCTCCCCACCTCCCAAAGGTCTACCGAGCTGGGGAGGAGGTACAGTAGGCCCT GTCCTGTCCTGTTTCTACAGGAAGTCATGCTCGAGGGAGTGTGAAGTGGTTCAGGTTGGT GCAGAGGCGCTCATGGCCTCCTGCTTCTTGCCTACCACTTGGCCAGTGCCCACCCAGCCC CTCAGGTGGCACATCTGGAGGGCAGGGGTTGAGGGGCCACCACCACACATGCCTTTCTGG GGTGAAGCCCTTTGGCTGCCCCACTCTCCTTGGATGGGTGTTGCTCCCTTATCCCCAAAT CACTCTATACATCCAATTCAGGAAACAAACATGGTGGCAATTCTACACAAAAAGAGATGA GATTAACAGTGCAGGGTTGGGGTCTGCATTGGAGGTGCCCTATAAACCAGAAGAGAAAAT ACTGAAAGCACAGGGGCAGGGACAGACCAGACCAGACCCAGGAGTCTCCAAAGCACAGAG TGGCAAACAAAACCCGAGCTGAGCATCAGGACCTTGCCTCGAATTGTCTTCCAGTATTAC GGTGCCTCTTCTCTGCCCCCTTTCCCAGGGTATCTGTGGGTTGCCAGGCTGGGGAGGGCA ACCATAGCCACACCACAGGATTTCCTGAAAGTTTACAATGCAGTAGCATTTTGGGGTGTA GGGTGGCAGCTCCCCAAGGCCCTGCCCCCCAGCCCCACCCACTCATGACTCTAAGTGTGT TGTATTAATATTTATTTATTTGGAGATGTTATTTATTAGATGATATTTATTGCAGAATTT CTATTCTTGTATTAACAAATAAAATGCTTGCCCCAGAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_013246 unedited
NGAGCTTGGCATTTGTAATACGACTTCCTATAGGGCGGCCGCGACTTCCCGGGGTATCGT CGACCCACGCGTCCGGCCGCGGCTCGCCCTCCCACTCCGCCAGCCTCCGGGAGAGGAGCC GCACCCGGCCGGCCCGGCCCCAGCCCCATGGACCTCCGAGCAGGGGACTCGTGGGGGATG TTAGCGTGCCTGTGCACGGTGCTCTGGCACCTCCCTGCAGTGCCAGCTCTCAATCGCACA GGGGACCCAGGGCCTGGCCCCTCCATCCAGAAAACCTATGACCTCACCCGCTACCTGGAG CACCAACTCCGCAGCTTGGCTGGGACCTATCTGAACTACCTGGGCCCCCCTTTCAACGAG CCAGACTTCAACCCTCCCCGCCTGGGGGCAGAGACTCTGCCCAGGGCCACTGTTGACTTG GAGGTGTGGCGAAGCCTCAATGACAAACTGCGGCTGACCCAGATCTACGAGGCCTACAGC CACCTTCTGTGTTACTTGCGTGGCCTCAACCGTCAGGCTGCCACTGCTGAGCTGCGCCGC AGCCTGGCCCACGGCTGCACCAGCCTCCAGGGCCTGCTGGGCAGCATTGCGGGCGTCATG GCAGCTCTGGGCTAACCACTGCCCCAGCCGCTGCCTGGGACTGAACCCACTTGGACTCCT GGCCCTGCCACAGTGACTTCCCTCCAAAAGATGGACAACTTNCTGGCTGCTAGAGGGAGC TGCAAGACCTGGCCTGTGGGCGCTCGGGCCAAGGGATTTCACCCGGCTCAAGAAAGAATA TTCAGNCCTCCAACAGCTGGAGGTACCCTGTGCCCTGGGGGCCTAAGGGTTTCTGACTTC TGAACTTTTCCTTTTTGCCTCCCCCTTAAAACCCTGGTCCCCTTTTGGAGAAACCCACCC CTGGTTGGCAACCCCTGTTGGAN |
| Restriction Sites | Please inquire |
| ACCN | NM_013246 |
| Insert Size | 1736 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013246.2, NP_037378.1 |
| RefSeq Size | 1860 bp |
| RefSeq ORF | 678 bp |
| Locus ID | 23529 |
| UniProt ID | Q9UBD9 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway |
| Gene Summary | This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009] Transcript Variant: This variant (1) encodes the longer isoform (1). Though this isoform has a predicted signal peptide at amino acids 1-27, experiments show it is retained within the cell. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Differential secretion of the mutated protein is a major component affecting phenotypic severity in CRLF1-associated disorders
,Jana Herholz, Alessandra Meloni, Mara Marongiu, Francesca Chiappe, Manila Deiana, Carmen Roche Herrero, Giuseppe Zampino, Hanan Hamamy, Yusra Zalloum, Per Erik Waaler, et al.,
European Journal of Human Genetics (16 February 2011) doi:10.1038/ejhg.2010.253 Article
[CLCF1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204427 | CLCF1 (Myc-DDK-tagged)-Human cardiotrophin-like cytokine factor 1 (CLCF1), transcript variant 1 |
CNY 2,400.00 |
|
| RC204427L1 | Lenti ORF clone of Human cardiotrophin-like cytokine factor 1 (CLCF1), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC204427L2 | Lenti ORF clone of Human cardiotrophin-like cytokine factor 1 (CLCF1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC204427L3 | Lenti ORF clone of Human cardiotrophin-like cytokine factor 1 (CLCF1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204427L4 | Lenti ORF clone of Human cardiotrophin-like cytokine factor 1 (CLCF1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG204427 | CLCF1 (tGFP-tagged) - Human cardiotrophin-like cytokine factor 1 (CLCF1), transcript variant 1 |
CNY 4,000.00 |
