United States
Germany
Japan
United Kingdom
China
Melanoma gp100 (PMEL) (NM_006928) Human Untagged Clone
CAT＃: SC122763
PMEL (untagged)-Human premelanosome protein (PMEL), transcript variant 3
CNY 7,392.00
| Cited in 3 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | D12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL17; SI; SIL; SILV |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_006928 edited
GAAGAACACAATGGATCTGGTGCTAAAAAGATGCCTTCTTCATTTGGCTGTGATAGGTGC TTTGCTGGCTGTGGGGGCTACAAAAGTACCCAGAAACCAGGACTGGCTTGGTGTCTCAAG GCAACTCAGAACCAAAGCCTGGAACAGGCAGCTGTATCCAGAGTGGACAGAAGCCCAGAG ACTTGACTGCTGGAGAGGTGGTCAAGTGTCCCTCAAGGTCAGTAATGATGGGCCTACACT GATTGGTGCAAATGCCTCCTTCTCTATTGCCTTGAACTTCCCTGGAAGCCAAAAGGTATT GCCAGATGGGCAGGTTATCTGGGTCAACAATACCATCATCAATGGGAGCCAGGTGTGGGG AGGACAGCCAGTGTATCCCCAGGAAACTGACGATGCCTGCATCTTCCCTGATGGTGGACC TTGCCCATCTGGCTCTTGGTCTCAGAAGAGAAGCTTTGTTTATGTCTGGAAGACCTGGGG CCAATACTGGCAAGTTCTAGGGGGCCCAGTGTCTGGGCTGAGCATTGGGACAGGCAGGGC AATGCTGGGCACACACACCATGGAAGTGACTGTCTACCATCGCCGGGGATCCCGGAGCTA TGTGCCTCTTGCTCATTCCAGCTCAGCCTTCACCATTACTGACCAGGTGCCTTTCTCCGT GAGCGTGTCCCAGTTGCGGGCCTTGGATGGAGGGAACAAGCACTTCCTGAGAAATCAGCC TCTGACCTTTGCCCTCCAGCTCCATGACCCTAGTGGCTATCTGGCTGAAGCTGACCTCTC CTACACCTGGGACTTTGGAGACAGTAGTGGAACCCTGATCTCTCGGGCACTTGTGGTCAC TCATACTTACCTGGAGCCTGGCCCAGTCACTGCCCAGGTGGTCCTGCAGGCTGCCATTCC TCTCACCTCCTGTGGCTCCTCCCCAGTTCCAGGCACCACAGATGGGCACAGGCCAACTGC AGAGGCCCCTAACACCACAGCTGGCCAAGTGCCTACTACAGAAGTTGTGGGTACTACACC TGGTCAGGCGCCAACTGCAGAGCCCTCTGGAACCACATCTGTGCAGGTGCCAACCACTGA AGTCATAAGCACTGCACCTGTGCAGATGCCAACTGCAGAGAGCACAGGTATGACACCTGA GAAGGTGCCAGTTTCAGAGGTCATGGGTACCACACTGGCAGAGATGTCAACTCCAGAGGC TACAGGTATGACACCTGCAGAGGTATCAATTGTGGTGCTTTCTGGAACCACAGCTGCACA GGTAACAACTACAGAGTGGGTGGAGACCACAGCTAGAGAGCTACCTATCCCTGAGCCTGA AGGTCCAGATGCCAGCTCAATCATGTCTACGGAAAGTATTACAGGTTCCCTGGGCCCCCT GCTGGATGGTACAGCCACCTTAAGGCTGGTGAAGAGACAAGTCCCCCTGGATTGTGTTCT GTATCGATATGGTTCCTTTTCCGTCACCCTGGACATTGTCCAGGGTATTGAAAGTGCCGA GATCCTGCAGGCTGTGCCGTCCGGTGAGGGGGATGCATTTGAGCTGACTGTGTCCTGCCA AGGCGGGCTGCCCAAGGAAGCCTGCATGGAGATCTCATCGCCAGGGTGCCAGCCCCCTGC CCAGCGGCTGTGCCAGCCTGTGCTACCCAGCCCAGCCTGCCAGCTGGTTCTGCACCAGAT ACTGAAGGGTGGCTCGGGGACATACTGCCTCAATGTGTCTCTGGCTGATACCAACAGCCT GGCAGTGGTCAGCACCCAGCTTATCATGCCTGGTCAAGAAGCAGGCCTTGGGCAGGTTCC GCTGATCGTGGGCATCTTGCTGGTGTTGATGGCTGTGGTCCTTGCATCTCTGATATATAG GCGCAGACTTATGAAGCAAGACTTCTCCGTACCCCAGTTGCCACATAGCAGCAGTCACTG GCTGCGTCTACCCCGCATCTTCTGCTCTTGTCCCATTGGTGAGAATAGCCCCCTCCTCAG TGGGCAGCAGGTCTGAGTACTCTCATATGATGCTGTGATTTTCCTGGAGTTGACAGAAAC ACCTATATTTCCCCCAGTCTTCCCTGGGAGACTACTATTAACTGAAATAAATACTCAGAG CCTGAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_006928 unedited
AAAAAATGTCACAATTGTATACGACTCATATAGGCGGCACGCGAATTCGCACGAGGGAAG AACACAATGGATCTGGTGCTAAAAAGAGCCTTCTTCATTTGGCTGTGATAGGTGCTTTGC TGGCTGTGGGGGCTACAAAAGTACCCAGAAACCAGGACTGGCTTGGTGTCTCAAGGCAAC TCATAACCAAAGCCTGGAACAGGCAGCTGTATCCAGAGTGGACAGAAGCCCAGAGACTTG ACTGCTGGAGAGGTGGTCAAGTGTCCCTCAAGGTCAGTAATGATGGGCCTACACTGATTG GTGCAAATGCCTCCTTCTCTATTGCCTTGAACTTCCCTGGAAGCCAAAAGGTATTGCCAG ATGGGCAGGTTATCTGGGTCAACAATACCATCATCAATGGGAGCCAGGTGTGGGGAGGAC AGCCAGTGTATCCCCAGGAAACTGACGATGCCTGCATCTTCCCTGATGGTGGACCTTGCC CATCTGGCTCTTGGTCTCAGAAGAGAAGCTTTGTTTATGTCTGGAAGACCTGGGGCCAAT ACTGGCAAGTTCTAGGGGGCCCAGTGTCTGGGCTGAGCATTGGGACAGGCAGGGCAATGC TGGGCACACACACCATGGAAGTGACTGTCTACCATCGCCGGGGATCCCGGAGCTATGTGC CTCTTGCTCATTCCAGCTCAGCCTTCACCATTACTGACCAGGTGCCTTTCTCCGTGAGCG TGTCCCAGTTGCGGGCCTTGGATGGAGGGAACAAGCACTTCCTGAGAAATCAGCCTCTGA CCTTTGCCCTNCAGCTNCATGACCCTAGTGGCTATCTGGCTGAAGCTGACCTCTCCTACA CCTGGGACTTTGGAGACAGTAGTGGAACCCTG |
| Restriction Sites | Please inquire |
| ACCN | NM_006928 |
| Insert Size | 2134 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006928.3, NP_008859.1 |
| RefSeq Size | 2143 bp |
| RefSeq ORF | 1986 bp |
| Locus ID | 6490 |
| UniProt ID | P40967 |
| Protein Families | Secreted Protein, Transmembrane |
| Gene Summary | This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011] Transcript Variant: This variant (3) has a longer 5' UTR and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (3, Pmel17-i) is shorter than isoform 1. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Mass Spectrometry and Imaging Analysis of Nanoparticle-Containing Vesicles Provide a Mechanistic Insight into Cellular Trafficking
,Hofmann, D;Tenzer, S;Bannwarth, MB;Messerschmidt, C;Glaser, SF;Schild, H;Landfester, K;Mailänder, V;,
ACS Nano
,PubMed ID 25244389
[PMEL]
|
|
The alarmin HMGN1 contributes to anti-tumor immunity and is a potent immunoadjuvant
,Wei, F;Yang, D;Tewary, P;Li, Y;Li, S;Chen, X;Howard, OM;Bustin, M;Oppenheim, JJ;,
Cancer Res.
,PubMed ID 25205103
[PMEL]
|
|
Artificial antigen-presenting cells expressing CD80, CD70, and 4-1BB ligand efficiently expand functional T cells specific to tumor-associated antigens
,Zeng, W;Su, M;Anderson, KS;Sasada, T;,
Immunobiology
[PMEL]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200663 | PMEL (Myc-DDK-tagged)-Human premelanosome protein (PMEL), transcript variant 3 |
CNY 7,384.00 |
|
| RC200663L1 | Lenti ORF clone of Human premelanosome protein (PMEL), transcript variant 3, Myc-DDK-tagged |
CNY 9,784.00 |
|
| RC200663L2 | Lenti ORF clone of Human premelanosome protein (PMEL), transcript variant 3, mGFP tagged |
CNY 9,784.00 |
|
| RC200663L3 | Lenti ORF clone of Human premelanosome protein (PMEL), transcript variant 3, Myc-DDK-tagged |
CNY 9,784.00 |
|
| RC200663L4 | Lenti ORF clone of Human premelanosome protein (PMEL), transcript variant 3, mGFP tagged |
CNY 9,784.00 |
|
| RG200663 | PMEL (tGFP-tagged) - Human premelanosome protein (PMEL), transcript variant 3 |
CNY 8,984.00 |
其它Melanoma gp100产品
