WDR59 (BC004519) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ12270; FP977; MGC11230; WD repeat domain 59
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC004519 edited CCGGGATACTGGGGACGGCGGGGTGGGAGGGCGCCGTCCTGGGGCCGCGGCGGCCGGGCG GGGGAGATGGCGGCGCGATGGAGCAGCGAAAACGTGGTTGTAGAGTTCCGTGACTCCCAG GCAACTGCGATGTCTGTGGACTGTCTTGGGCAGCATGCAGTGCTTTCTGGCCGCAGATTC TTATACATCGTCAATCTAGATGCCCCTTTCGAAGGTCACCGAAAGATCTCTCGCCAGAGC AAATGGGACATTGGAGCTGTGCAGTGGAATCCTCATGACAGCTTTGCACACTATTTTGCG GCTTCGAGTAACCAACGAGTAGACCTTTACAAGTGGAAAGACGGCAGTGGGGAAGTTGGC ACAACCTTACAAGGCCACACTCGTGTCATCAGCGACTTGGACTGGGCGGTGTTTGAGCCT GACCTCCTGGTTACCAGCTCTGTGGACACCTACATCTACATTTGGGATATCAAAGACACA AGGAAACCTACTGTTGCACTGTCTGCTGTTGCGGGTGCCTCCCAGGTCAAATGGAATAAA AAAAATGCTAACTGCCTTGCCACCAGCCATGACGGCGATGTGCGGATATGGGATAAGAGG AAACCCAGTACAGCAGTGGAATATCTAGCCGCCCACCTCTCCAAAATCCATGGCCTGGAC TGGCACCCAGACAGCGAGCACATTCTTGCTACCTCCAGTCAAGACAATTCTGTGAAGTTC TGGGATTACCGCCAGCCTCGGAAATACCTCAATATTCTTCCTTGCCAGGTGCCTGTCTGG AAGGCCAGATACACACCTTTCAGCAATGGATTGGTGACTGTGATGGTTCCCCAGCTGCGG AGGGAAAACAGCCTTCTCCTGTGGAATGTCTTTGACTTGAACACCCCAGTCCACACCTTC GTGGGGCATGATGATGTGGTCCTGGAGTTCCAGTGGAGGAAGCAGAAGGAAGGGTCCAAG GACTATCAACTGGTGACGTGGTCCCGGGATCAGACCTTGAGAATGTGGCGGGTGGATTCC CAGATGCAGAGGCTTTGTGCAAATGACATATTAGATGGTGTTGATGAGTTCATTGAGAGT ATTTCCCTTCTGCCGGAACCTGAGAAGACCCTGCACACTGAAGATACAGATCACCAGCAC ACTGCAAGCCATGGGGAGGAAGAAGCCCTAAAAGAAGATCCCCCTAGAAATCTCCTGGAA GAGAGGAAATCAGATCAACTGGGGCTGCCTCAGACCTTGCAGCAGGAATTCTCCCTGATC AATGTGCAAATCCGGAATGTCAATGTGGAGATGGATGCGGCAGACAGGAGCTGCACAGTG TCTGTGCACTGCAGCAACCATCGTGTCAAGATGCTGGTGAAGTTCCCTGCACAGTACCCA AACAACGCCGCCCCTTCCTTCCAGTTTATTAACCCCACAACCATCACATCCACCATGAAA GCTAAGCTGCTGAAGATCCTGAAGGACACAGCCCTGCAGAAAGTGAAGCGTGGCCAGAGC TGCCTGGAGCCCTGCCTGCGCCAGCTCGTCTCCTGCCTTGAGTCCTTTGTGAACCAGGAA GACAGCGCTTCCAGCAACCCGTTTGCACTCCCCAACTCTGTCACTCCCCCCTTACCGACG TTTGCGCGGGTGACCACGGCTTACGGGTCGTACCAGGACGCCAACATTCCCTTTCCTAGG ACTTCTGGGGCCAGGTTCTGCGGAGCAGGTTACCTGGTATATTTCACAAGGCCCATGACA ATGCATCGGGCGGTGTCTCCCACAGAGCCTACTCCGAGGTGAGTCAGGGAGATGCAGAAG CCGCTCTACGTTCCCGGGGCCAGAAGTGGCCAGAGACCTGGGAAGGACCACTGAGTTTGC TTTTGGACAGTGCATTTCAGTTCTTCAGTAAACTCCCATTATAAGATTTATTTGAGAAAG ATTGAAGTCAATTAAATTGCAAAAAAGCTGTGAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC004519 unedited NNCCAGTGTCGGGATTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGCACGAGGC CGGGATACTGGGGACGGCGGGGTGGGAGGGCGCCGTCCTGGGGCCGCGGCGGCCGGGCGG GGGAGATGGCGGCGCGATGGAGCAGCGAAAACGTGGTTGTAGAGTTCCGTGACTCCCATG CAACTGCGATGTCTGTGGACTGTCTTGGGCAGCATGCAGTGCTTTCTGGCCGCAGATTCT TATACATCGTCAATCTAGATGCCCCTTTCGAAGGTCACCGAAAGATCTCTCGCCAGAGCA AATGGGACATTGGAGCTGTGCAGTGGAATCCTCATGACAGCTTTGCACACTATTTTGCGG CTTCGAGTAACCAACGAGTAGACCTTTACAAGTGGAAAGACGGCAGTGGGGAAGTTGGCA CAACCTTACAAGGCCACACTCGTGTCATCAGCGACTTGGACTGGGCGGTGTTTGAGCCTG ACCTCCTGGTTACCAGCTCTGTGGACACCTACATCTACATTTGGGATATCAAAGACACAA GGAAACCTACTGTTGCACTGTCTGCTGTTGCGGGTGCCTCCCANGTCAAATGGAATAAAA AAAATGCTAACTGCCTTGCCACCAGCCATGACGGCGATGTGCGGATATGGGATAAGATGA AACCCAGTACAGCAGTGGAATATCTAGCCGCCCACCTCTCCAAAATCATGGCCTGNACTG GCACCCAGACAGCGAGCACATTCTTGCTACCTCCAGTCAAGACAATTCTGTGAAGTTCTG GGATTACCGNCAGCCTCGGAAATACCTCAATATTCTTNCTTGCCAGGTGCCTGTCTGGAA TGCCAGATACACACCTTTCAGCATGGNATGGTGACTGTGATGGTTCCCCAGCTGCGGA
Restriction Sites	Please inquire
ACCN	BC004519
Insert Size	1985 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC004519.2, AAH04519.1
RefSeq Size	1976 bp
Locus ID	79726
Gene Summary	As a component of the GATOR subcomplex GATOR2, functions within the amino acid-sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex (PubMed:23723238). It is negatively regulated by the upstream amino acid sensors SESN2 and CASTOR1 (PubMed:25457612, PubMed:27487210).[UniProtKB/Swiss-Prot Function]

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