MITF (BC012503) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bHLHe32; CMM8; MI; WS2; WS2A
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC012503 edited CTTTGCCAGTCCATCTTCAAATTGGAATTATAGAAAGTAGAGGGAGGGATAGTCTACCGT CTCTCACTGGATTGGTGCCACCTAAAACATTGTTATGCTGGAAATGCTAGAATATAATCA CTATCAGGTGCAGACCCACCTCGAAAACCCCACCAAGTACCACATACAGCAAGCCCAACG GCAGCAGGTAAAGCAGTACCTTTCTACCACTTTAGCAAATAAACATGCCAACCAAGTCCT GAGCTTGCCATGTCCAAACCAGCCTGGCGATCATGTCATGCCACCGGTGCCGGGGAGCAG CGCACCCAACAGCCCCATGGCTATGCTTACGCTTAACTCCAACTGTGAAAAAGAGTTTAT GAAGCAGTGAGAATGCAGAGAGAGGAGAAGGGGAGGTGGAAAAGGAAAAGCAAAAATAGA AGAGGTGTGGGACATGCTGTTTAGAAGTTCCGCTTGTTGTGAATGTCTGGAATATTATTT TTATTTCTCCCTGAGTTGGGGGAAGAAAGAATGGAATATGCATGGATGGATTTGAATCAT ATAGCACATGAGACTTTAACGGAAACGCAAAGGTTTAATTGCTGGATACATTCTGTTTCA TAATAAAATTGCCACTGCCCGTTAAATCTGCTTTGGTGAAGGCTGGATTGGAAACAAGAC TCAAACTACCTTCAAGCTAATTGGTGCATCAAAATTTGCAGCATACAAATACCTGAGAGC TGTGATTTAATGCTCATTATTTCCAAATTATGAGATGATGAGCTTCATCTCAATGGGATT TACCGTACTATGGACTATGAAGTGTTTATGCAAATTCGGAGGCAACTTTTCTAGAGTTGG ATTGATTTTAATTTCTAGAGGGACTAAAATCTTTGCCCCTATGCCCAAACCAACTGCTTT ATTTTTCTCTACCCAAATTTGTCATCTAGCAAGATGATTTGACACAAGTTCTTCCTTCAT TATTTCATCTTTTGGTCAGATTCCACTTTGTTTGAAAGCTTAGTTCATCTTGTTGCTGTG CCATCAGCTTTGTGTGAACAGGTCATTAAAAAGTCATTTGCAAATCCAAAAAAAAAAAAA AA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC012503 unedited GGGCGGTTCAAATTTGTATACGACTCACTATGGGCGGCCGCGATTCCCGGGATATCGTCG ACCCACGCGTCCGCTTTGCCAGTCCATCTTCAAATTGGAATTATAGAAAGTAGAGGGAGG GATAGTCTACCGTCTCTCACTGGATTGGTGCCACCTAAAACATTGTTATGCTGGAAATGC TAGAATATAATCACTATCAGGTGCAGACCCACCTCGAAAACCCCACCAAGTACCACATAC AGCAAGCCCAACGGCAGCAGGTAAAGCAGTACCTTTCTACCACTTTAGCAAATAAACATG CCAACCAAGTCCTGAGCTTGCCATGTCCAAACCAGCCTGGCGATCATGTCATGCCACCGG TGCCGGGGAGCAGCGCACCCAACAGCCCCATGGCTATGCTTACGCTTAACTCCAACTGTG AAAAAGAGTTTATGAAGCAGTGAGAATGCAGAGAGAGGAGAAGGGGAGGTGGAAAAGGAA AAGCAAAAATAGAAGAGGTGTGGGACATGCTGTTTAGAAGTTCCGCTTGTTGTGAATGTC TGGAATATTATTTTTATTTCTCCCTGAGTTGGGGGAAGAAAGAATGGAATATGCATGGAT GGATTTGAATCATATAGCACATGAGACTTTAACGGAAACGCAAAGGTTTAATTGCTGGAT ACATTCTGTTTCATAATAAAATTGCCACTGCCCGTTAAATCTGCTTTGGTGAAGGCTGGA TTGGAAACAAGACTCANACTACCTTCAAGCTAATTGGTGCATCAAAATTTGCAGCATACA AATACCTGAGAGCTGTGATTTAATGCTCATTATTTCCAATTATGAGATGATGAGCTTCAT CTCAATGGGATTTACCGTACTATGGGACTATGAAGTGTTTATGCCAATTCGGAGGCAACT TTNCTAGATTGGNA
Restriction Sites	Please inquire
ACCN	BC012503
Insert Size	1082 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC012503.1
RefSeq Size	1082 bp
Locus ID	4286
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Melanogenesis, Melanoma, Pathways in cancer
Gene Summary	The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017]

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