RNF158 (SH3RF2) (BC073914) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FLJ23654; MGC90410; MGC149788; MGC149789; RNF158; SH3 domain containing ring finger 2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC073914 edited GCTGCCACGTCCGGAGGAGGGCAGCAAGAATGAAAGACCTCTAGTTTTCCAGACTCCCGG AGCCCTGGTCTCTACACCACATGGACGTTATCCACCTCCTCTGTGTCCTCCCAAGGCAGC ATTTCAGAAGGTGATCCACGGCAAAGCCGTCCCTTCAAATCCGTCTTTGTGCCCACTGCC ATAGTCAACCCCGTGAGAAGCACAGCCGGCCCTGGGACTTTAGGACAAGGGTCTCTTCGG AAAGGGCGGAGCAGCATGAGAAAGAGTAAGTGGTGGCAGAGAGATGGATCCCTGCAGAGA CCCCTCCAGTCCGGGATCCCCACTCTCGTGGTAGGCTCCCTCAGACGCAGCCCCACCATG GTCCTTCGGCCTCAGCAGTTCCAATTCTACCAGCCACAGGGGATCCCCTCCTCCCCCTCA GCCGTGGTGGTGGAGATGGGGTCCAAGCCTGCCCTCACGGGGGAGCCCGCCCTCACGTGC ATCAGCAGGGGCAGTGAGGCCCGGATCCACTCCGCGGCCAGCTCCCTCATTATGGAAGAC AAAGAAATCCCCATCAAGAGTGAGCCTCTGCCAAAACCGCCCGCATCTGCCCCACCATCC ATCCTGGTGAAACCAGAAAACTCAAGAAATGGCATCGAAAAGCAAGTCAAAACCGTGAGA TTTCAGAATTACAGCCCTCCTCCCACCAAACATTACACCTCCCATCCCACCTCCGGAAAG CCTGAACAGCCAGCCACCCTCAAGGCGTCCCAGCCTGAAGCAGCGTCCTTGGGCCCAGAG ATGACCGTCCTATTTGCCCACCGAAGTGGCTGCCACTCCGGACAGCAGACAGACCTCCGG AGAAAGTCAGCTCTTGCCAAGGCCACAACCCTGGTGTCCACTGCCTCAGGCACGCAGACC GTGTTTCCCAGCAAATGAACCTACGGGTGGCTTTTCCTAGACCCCAAAGAGGTGAATTGC ATTTAAATACAGTCTGCCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC073914 unedited TTAATGGGATTTGNNAAACGACATANTATAGGCGGCCACGCCTTCCCGGGNAGCTGCCAC GTCCTTTAGAGGGCAGCAAGAATGAAGAGACTCTAGTTTTCCAGACTCCCGGAGCCCTGG TCTCTACACCACATGGACGTTATCCACCTCCTCTGTGTCCTCCCAAGGCAGCATTTCAGA AGGTGATCCACGGCAAAGCCGTCCCTTCAAATCCGTCTTTGTGCCCACTGCCATAGTCAA CCCCGTGAGAAGCACAGCCGGCCCTGGGACTTTAGGACAAGGGTCTCTTCGGAAAGGGCG GAGCAGCATGAGAAAGAGTAAGTGGTGGCAGAGAGATGGATCCCTGCAGAGACCCCTCCA GTCCGGGATCCCCACTCTCGTGGTAGGCTCCCTCAGACGCAGCCCCACCATGGTCCTTCG GCCTTAGCAGTTCCAATTCTACCAGCCACAGGGGATCCCCTCCTCCCCCTCAACCGTGGT GGTGGAGATGGGGTCCAAGCCTGCCCTCACGGGGGAGCCCGCCCTCACGGTGCATTATCA AGGGCAGTGATGCCCGGATCCACTCCGCGGGCAGCTCCCTCATTATGGACACAAACAAAT CCCATCAAAGTGAGCCTCTGCCAAAACCGCCCGGATTCTGCCCAACCTCCATCCTGGGGA AACCAGGAAACTGACGAAATGGCCTTCTAAAAGCAAGTCACACCCTTGGGATTTCATAAA TACAGCCGTCTGCCACCAAAAATTAACACTGCCATTCCCACTTACAGAAAGTCCTGAAAA GCCAACCACCCCTTCAGGGCTTCCAAGCTTGAGAGCAAGCAGTCTTGGACCCCACAAAGA GACCGTCTCAATTAGCTCACCGAATTTGTCCGTCCTATATCGGTGCTGATATAACGAACT CTTCCGATAAAATTCGACTCTTTTTGCCACAGGCCCTGACCCAATGGAGTTCCCAGT
Restriction Sites	Please inquire
ACCN	BC073914
Insert Size	1058 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC073914.1, AAH73914.1
RefSeq Size	1065 bp
Locus ID	153769
Gene Summary	Has E3 ubiquitin-protein ligase activity (PubMed:24130170). Acts as an anti-apoptotic regulator of the JNK pathway by ubiquitinating and promoting the degradation of SH3RF1, a scaffold protein that is required for pro-apoptotic JNK activation (PubMed:22128169). Facilitates TNF-alpha-mediated recruitment of adapter proteins TRADD and RIPK1 to TNFRSF1A and regulates PAK4 protein stability via inhibition of its ubiquitin-mediated proteasomal degradation (PubMed:24130170). Inhibits PPP1CA phosphatase activity (PubMed:19945436, PubMed:19389623).[UniProtKB/Swiss-Prot Function]

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