CLYBL (BC034360) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA134O15.1; citrate lyase beta like; CLB; OTTHUMP00000018626; OTTHUMP00000018628; OTTHUMP00000040737
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC034360 edited CGCGCGTCGGGAAGATGGCGCTACGTCTGCTGCGGAGGGCGGCGCGCGGAGCTGCGGCGG CGGCGCTGCTGAGGCTGAAAGCGTCTCTAGCAGCTGATATCCCCAGACTTGGATATAGTT CCTCATCCCATCACAAGTACATCCCCCGGAGGGCAGTGCTTTATGTACCTGGAAATGATG AAAAGAAAATAAAGAAGATTCCATCCCTGAATGTAGATTGTGCAGTGCTCGACTGTGAGG ATGGAGTGGCTGCAAACAAAAAGAATGAAGCTCGACTGAGAATTGTAAAAACTCTTGAAG ACATTGATCTGGGCCCTACTGAAAAATGTGTGAGAGTCAACTCAGTTTCCAGTGGTCTGG CGGAAGAAGACCTAGAGACCCTTTTGCAATCCCGGGTCCTTCCTTCCAGCCTGATGCTAC CAAAGGTGGAAAGTCCTGAAGAAATCCAGTGGTTTGCAGACAAATTTTCATTCCACTTAA AAGGCCGAAAACTTGAACAACCAATGAATTTAATCCCTTTTGTGGAAACTGCAATGGGTT TGCTCAATTTTAAGGCAGTGTGTGAAGAAACCCTGAAGGTCGGGCCTCAAGTAGGTCTCT TTCTAGATGCAGTCGTTTTTGGAGGAGAAGACTTTCGAGCCAGCATAGGTGCAACAAGTA GTAAAGAAACCCTGGATATTCTCTACGCCCGGCAAAAGATTGTTGTCATAGCGAAAGCCT TTGGTCTCCAAGCCGTAGATCTGGTGTACATTGACTTTCGAGATGGAGCTGGGCTGCTTA GACAGTCACGAGAAGGAGCCGCCATGGGCTTCACTGGTAAGCAGGTGATTCACCCTAACC AAATTGCCGTGGTCCAGGAGCAGTTTTCTCCTTCCCCTGAAAAAATTAAGTGGGCTGAAG AACTGATTGCTGCCTTTAAAGAACATCAACAATTAGGAAAGGGGGCCTTTACTTTCCAAG GGAGTATGATCGACATGCCATTACTGAAGCAGGCCCAGAACACTGTTACGCTTGCCACCT CCATCAAGGAAAAATGATCTGTTAAATGAAGCTGTCATCAGGCTAAAGGGTATTGAAGCT GCAGAGGGATCAACTTGTGCTTGCCAGAGGACGCCAATGAAGTTTGAAACACCAACAATC AGAGATTTTGTTTCTGTTCCTCATTAAATCATGAGCTTTTGTGCCGAGAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC034360 unedited CAAATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCCCGGGATCGCGCGTCGGGAA GATGGCGCTACGTCTGCTGCGGAGGGCGGCGCGCGGAGCTGCGGCGGCGGCGCTGCTGAG GCTGAAAGCGTCTCTAGCAGCTGATATCCCCAGACTTGGATATAGTTCCTCATCCCATCA CAAGTACATCCCCCGGAGGGCAGTGCTTTATGTACCTGGAAATGATGAAAAGAAAATAAA GAAGATTCCATCCCTGAATGTAGATTGTGCAGTGCTCGACTGTGAGGATGGAGTGGCTGC AAACAAAAAGAATGAAGCTCGACTGAGAATTGTAAAAACTCTTGAAGACATTGATCTGGG CCCTACTGAAAAATGTGTGAGAGTCAACTCAGTTTCCAGTGGTCTGGCGGAAGAAGACCT AGAGACCCTTTTGCAATCCCGGGTCCTTCCTTCCAGCCTGATGCTACCAAAGGTGGAAAG TCCTGAAGAAATCCAGTGGTTTGCAGACAAATTTTCATTCCACTTAANAGGCCGAANACT TGAACAACCAATGAATTTAATCCCTTTTGTGGAAACTGCAATGGGTTTGCTCAATTTTAA GGCAGTGTGTGAAGAAACCCTGAAAGTCGGGNCCTCAGTANGTCTCTTTCTAGATGCAGT CGTTTTTGGGAGAGAAGAACTTCGAGCCAGCATANGTGCCAACAGTAGTAAAGAAACCCT GGNATATCTCTACGCCCCGCAAAGAATTGTGTCATAGCGAAAGCTTTTGGGTCTCAGCCG TAGATCCTGTGGACATTGACTTTNCAGATGGACCTGGGCTGCTTANACAGTCCCGAGAAG GAGCCCGCCTGGGCTTCACTGGTAAGCAAGTGAATCACCCTAACACATTGCCCTGGTCCA GGAGCAAGTTTCTCCTTCCCTGGAAAAATTAATGGGCTGAAAAATGGATG
Restriction Sites	Please inquire
ACCN	BC034360
Insert Size	1224 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC034360.1, AAH34360.1
RefSeq Size	1224 bp
Locus ID	171425
Gene Summary	Mitochondrial citramalyl-CoA lyase indirectly involved in the vitamin B12 metabolism (PubMed:29056341). Converts citramalyl-CoA into acetyl-CoA and pyruvate in the C5-dicarboxylate catabolism pathway (PubMed:29056341). The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite (PubMed:29056341). Also acts as a malate synthase in vitro, converting glyoxylate and acetyl-CoA to malate (PubMed:29056341, PubMed:24334609). Also displays malyl-CoA thioesterase activity (PubMed:29056341). Also acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate (PubMed:24334609, PubMed:29056341). Also has very weak citramalate synthase activity in vitro (PubMed:24334609, PubMed:29056341).[UniProtKB/Swiss-Prot Function]

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