HSH2D (BC025237) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	adaptor in lymphocytes of unknown function X; ALX; FLJ14886; hematopoietic SH2 domain containing; hematopoietic SH2 protein; HSH2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC025237 edited CCAGGTGACCTTCCCTCCACCCCAGGAAGCTATGACAGAGGCCGGGAAGCTGCCCCTACC GCTACCCCCACGGCTGGACTGGTTTGTGCACACCCAGATGGGCCAGCTGGCCCAAGACGG GGTCCCCGAGTGGTTCCATGGTGCAATCTCAAGAGAGGATGCTGAGAACTTGCTGGAGTC ACAGCCACTGGGATCCTTTCTCATCAGGGTCAGTCACAGCCATGTGGGCTACACACTCTC CTACAAAGCCCAAAGCAGCTGCTGCCATTTCATGGTGAAGCTCTTGGATGATGGGACTTT CATGATCCCCGGGGAGAAGGTGGCCCACACCTCGCTGGACGCCCTGGTCACCTTCCACCA GCAGAAGCCAATTGAGCCGCGCAGGGAGCTGCTGACACAGCCCTGCAGGCAGAAGGATCC CGCAAACGTGGATTACGAGGATCTCTTCCTCTACTCCAACGCAGTGGCCGAGGAAGCTGC CTGCCCGGTGTCTGCCCCTGAGGAGGCCTCCCCAAAGCCAGTCCTGTGTCACCAATCAAA GGAAAGGAAGCCGTCAGCAGAGATGAACAGAATAACCACCAAGGAAGCCACTTCCTCCTG CCCCCCAAAATCCCCTCTTGGAGAGACCCGCCAGAAACTCTGGAGGAGCCTCAAAATGCT CCCCGAGAGAGGCCAGAGGGTCCGGCAGCAGCTAAAAAGCCACCTCGCCACTGTGAACTT GTCGTCACTCTTGGATGTCCGGAGATCCACGGTGATCTCAGGCCCTGGGACCGGAAAAGG CAGCCAAGATCACTCAGGGGATCCCACCTCGGGGGACAGAGGCTACACGGATCCCTGTGT GGCCACATCTCTCAAAAGCCCCTCACAGCCCCAGGCACCAAAAGACAGAAAGGTCCCCAC CAGGAAGGCCGAGAGGTCGGTCAGCTGCATTGAGGTGACCCCAGGGGACAGGAGTTGGCA CCAAATGGTAGTGAGAGCCCTATCCTCCCAGGAGTCCAAGCCAGAGCACCAGGGCTTGGC AGAGCCTGAGAACGACCAGCTCCCGGAGGAGTACCAACAACCGCCACCCTTTGCCCCTGG GTACTGCTAGAGAACAGGTCCACCCTGGCTCTGGGACTCGCTGCCAGGGGCTGCCACACT CCTGAATGCCTTAACATTTCTTCCATGGCCCCACACCATGGCATCCGGGGGTCTTCGGGA ACCCGGGAAATGGAATAAAGATGTTTTTGGGGTCTGTTCCTGCACTCACCCATGGGGTGA GCTGGTTATTTTAGCAACAATCATCAGAGTGACGCTGATGGTTTGGGGCACCAGCTATAC ATCAGCCCCAGTGCCAGACCTTCTATTCATTATTTTACGCCTCAGAGCAAGGCCCTCAGG GAGGGTCATCCTCCATGTTTTGAAGAAGAGACTGAGGTTCAGAGAGGATAAGAGGCGTGA CCAAGGCCACAGAGCTATGGGTGTCAGCACCAGGATTTGAAGCCAGGTGAATCCGAGCCC TTTTCCCATATCATCTGTTTGTTCTGTTGTCTAAAAGCACACTGCAAGCCGGGCTCAGTG GCTCATGCCTGTAGTCCCAGCACTCTGTGGGGCCGAGGCAGGCAGATCGCTTGAGGTCAG GAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCCGTCTATACTAAAAAATTCAAAAA TTACCCGGACGTGGTGGCGCATGCCTGTAATCCCAGCTACTTGGGAGCCTGAGGCGGGAG AATTGCTTGAACCCGGGAGGCAGAGGTTGCAGTGAGCCGAGATCGCATCACTGCAGTCCA GCCTGGATGACAGAGTGAGACTCCATCTCAAAAAATAAATAAATAAATAAAAATGAAATT AAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC025237 unedited NAAGTCAACATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGAGGGCCT CGGCCATCCAAGGTCTCCCAGTGACCTTCCCTCCACCCCAGGAAGCTATGACAGAGGCCG GGAAGCTGCCCCTACCGCTACCCCCACGGCTGGACTGGTTTGTGCACACCCAGATGGGCC AGCTGGCCCAAGACGGGGTCCCCGAGTGGTTCCATGGTGCAATCTCAAGAGAGGATGCTG AGAACTTGCTGGAGTCACAGCCACTGGGATCCTTTCTCATCAGGGTCAGTCACAGCCATG TGGGCTACACACTCTCCTACAAAGCCCAAAGCAGCTGCTGCCATTTCATGGTGAAGCTCT TGGATGATGGGACTTTCATGATCCCCGGGGAGAAGGTGGCCCACACCTCGCTGGACGCCC TGGTCACCTTCCACCAGCAGAAGCCAATTGAGCCGCGCAGGGAGCTGCTGACACAGCCCT GCAGGCAGAAGGATCCCGCAAACGTGGATTACGAGGATCTCTTCCTCTACTCCAACGCAG TGGCCGAGGAAGCTGCCTGCCCGGTGTCTGCCCCTGAGGAGGCCTCCCCAAAGCCAGTCC TGTGTCACCAATCAAAGGAAAGGAAGCCGTCAGCAGAGATGAACAGAATAACCACCAAGG AAGCCACTTCCTCCTGCCCCCCAAAATCCCCTCTTGGAGAGACCCGCCAGAAACTCTGGA GGAGCCTCAAAATGCTCCCCGAGAGAGGCCAGAGGGTCCGGCAGCAGCTAAAAAGCCACC TCGCCACTGTGAACTTGTCGTCACTCTTGGATGTCCGGAGATCCACGGTGATCTCAGGCC CTGGGACGGNAAATGCAGCCAAAGATCACTCAGGGATCCCCACTCGGGGGACAGAG
Restriction Sites	Please inquire
ACCN	BC025237
Insert Size	1928 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC025237.2, AAH25237.1
RefSeq Size	1903 bp
Locus ID	84941
Gene Summary	T-cell activation requires 2 signals: recognition of antigen by the T-cell receptor (see TCR; MIM 186880) and a costimulatory signal provided primarily by CD28 (MIM 186760) in naive T cells. HSH2 is a target of both of these signaling pathways (Greene et al., 2003 [PubMed 12960172]).[supplied by OMIM, Mar 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.