OTUB2 (BC009615) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C14orf137; FLJ21916; MGC3102; OTB2; OTU2; OTU domain, ubiquitin aldehyde binding 2; ubiquitin-specific protease otubain 2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC009615 edited CTTTGGTTTGCGGAGCGGTCGGGTGTATTCTCCGCCGCCCCCACGCCCTCGAGGTCCCCG CCACCGAACCAGCGGCGGAGCCCGCCCGCGCCTCCCGCGGCATTCCCGCACCGGATCGCT CCTCGCTGGGGCGGGACCTGGCCTGGCGGCTCTGGTCACTATGAGTGAAACATCTTTCAA CCTAATATCAGAAAAATGTGACATTCTATCCATTCTTCGGGACCATCCTGAAAACAGGAT TTACCGGAGGAAAATCGAGGAACTCAGCAAAAGGTTCACCGCCATCCGCAAGACCAAAGG GGATGGGAACTGCTTCTACAGGGCCTTGGGCTATTCCTACCTGGAGTCCCTGCTGGGGAA GAGCAGGGAGATCTTCAAGTGAGTGCCGGGGCCCCTTGGCCTGTCAGGGCTGTGTTCTCT GTGCTAGGTCAGCCTCAAGTCTCTCGGAGGTTTTCGTTACACTCAGGCTGGTTAACCCAG AGAATGAGCTGAAGAGCTGCCTGGCAGAGGCGTTTGATGTTGGGGGTGCCCAGCTGCCTG CTGTGGTGAAGTTGGGGGCAGCCAAGGGGTGGAGGATGGAATCTGTGACCTCCCAGATGC TTTCCAGCCTCTGCTTCCACCACAGGCCTGGGTCAGCCCAGGTGAGTCGCAGGGAGCAGG TACTTCGTGACCCACTCTGAAAAGAAAAGAAAAGACAAACAAAAAGAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC009615 unedited NCCCGAGCTTTATTTGTAAACGACTTATATAGGCGGCCGCGNAATCCCGGGNAATCGTCG ACCCACGCGTCCGCTTTGGTTTGCGGAGCGGTCGGGTGTATTCTCCGCCGCCCCCACGCC CTCGAGGTCCCCGCCACCGAACCAGCGGCGGAGCCCGCCCGCGCCTCCCGCGGCATTCCC GCACCGGATCGCTCCTCGCTGGGGCGGGACCTGGCCTGGCGGCTCTGGTCACTATGAGTG AAACATCTTTCAACCTAATATCAGAAAAATGTGACATTCTATCCATTCTTCGGGACCATC CTGAAAACAGGATTTACCGGAGGAAAATCGAGGAACTCAGCAAAAGGTTCACCGCCATCC GCAAGACCAAAGGGGATGGGAACTGCTTCTACAGGGCCTTGGGCTATTCCTACCTGGAGT CCCTGCTGGGGAAGAGCAGGGAGATCTTCAAGTGAGTGCCGGGGCCCCTTGGCCTGTCAG GGCTGTGTTCTCTGTGCTAGGTCAGCCTCAAGTCTCTCGGAGGTTTTCGTTACACTCAGG CTGGTTAACCCAGAGAATGAGCTGAAGAGCTGCCTGGCAGAGGCGTTTGATGTTGGGGGT GCCCAGCTGCCTGCTGTGGTGAAGTTGGGGGCAGCCAAGGGGTGGAGGATGGAATCTGTG ACCTCCCAGATGCTTTCCAGCCTCTGCTTCCACCACAGGCCTGGGTCAGCCCAGGTGAGT CGCAGGNAGCANGTACTTCGTGACCCACTCTGAAAAGAAAAGAAAAGACCAACCNNANGN ANAAAAANNAANNNNANANNNNAAAANNNNNNAAAAANNNNNNAAANAAAAANNAAAAAA AANAAAAAAANAAAAAAAAAGGCGGGCCCCGGTCATAGCTGTTTCCTGAACA
Restriction Sites	Please inquire
ACCN	BC009615
Insert Size	790 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC009615.1, AAH09615.1
RefSeq Size	790 bp
Locus ID	78990
Protein Families	Protease
Gene Summary	This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain. [provided by RefSeq, Aug 2011]

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