TTYH1 (BC019358) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	4930459B04Rik; 6330408P11Rik; OTTMUSP00000024583; tty; tweety 1; tweety homolog 1 (Drosophila)
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC019358 edited GTCCTGTCTCATGATAACACTATTTCACAGAGGACCTGGGCTGTGGCCCAGACACAGTAA ACAGCAGGAGGCTGAGCTTGGAGGGTGGTGAATGTGGCTGGCCTACGTCCTCCTGCTGCT CCTGGAGCTGCTGGTCTGCCTCTTCACCCTCCTGGGCCTGGCGAAGCAGAGCAAGTGGCT GGTGATCGTGATGACAGTCATGAGTCTCCTGGTTCTCGTCCTGAGCTGGGGCTCCATGGG CCTGGAGGCAGCCACGGCCGTGGGCCTCAGTGACTTCTGCTCCAATCCAGACCCTTATGT TCTGAACCTGACCCAGGAGGAGACAGGGCTCAGCTCAGACATCCTGAGCTATTATCTCCT CTGCAACCGGGCCGTCTCCAACCCCTTCCAACAGAGGCTGACTCTGTCCCAGCGAGCTCT GGCCAACATCCACTCCCAGCTGCTGGGCCTGGAGCGAGAAGCTGTGCCTCAGTTCCCTTC AGCGCAGAAGCCTCTGCTGTCCTTGGAGGAGACTCTGAATGTGACAGAAGGAAATTTCCA CCAGTTGGTGGCACTGCTACACTGCCGCAGCCTGCACAAGGACTATGGTGCAGCCCTGCG GGGCCTGTGCGAAGACGCCCTGGAAGGCCTGCTCTTCCTGCTGCTCTTCTCCCTGCTGTC TGCAGGAGCGCTGGCCACTGCCCTCTGCAGCCTGCCCCGAGCCTGGGCCCTCTTCCCACC CAGTGACGACTACGATGACACAGACGATGACGACCCTTTCAACCCTCAGCAGGAATCCAA GCGCTTTGTGCAGTGGCAGTCGTCTATCTGAGCCCCTCCTCCCGGCTGGACTGGAGCCTG GCTCCCCTCTTCGTTCCTTCCCTGGCTGCCGGAGGAGACCCCACTAACCCAGCCTGCCTG GGCTCTGACCACTAACACTCTTGGCCATGGACAGCCTGCACAGGACCGCCTCCCTGCTCT TGGCCACTGTGCTCCCATTTCTGTCCTTGGCCTTGGGAGTAGCTGAGGGGGCAGACTAGG GAGTAGGGCTGGCAGGGGAGGGGGCAGACAGCCTCGCCTCGCACCCTTCATCCCTGGCTG CCGGTCCCATCCTTGGAGGGACTAAGCTGGGGGTGGGGGACATGAGTCCCCCTGCTGCCC CTGCCACATCCCAGTGGGCTCTGACCCCCTGATCTCAACTCGTGGCACTAACTTGGAAAA GGGTTGATTTAAAATAAAAGGGAAGACTATTTTACAAGCAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC019358 unedited TAGTTAGAATTGTATACGACTTACTATAGGCGGCCGCGAATTCGCACGAGGTCCTGTCTC ATGATAACACTATTTCACAGAGGACCTGGGCTGTGGCCCAGACACAGTAAACAGCAGGAG GCTGAGCTTGGAGGGTGGTGAATGTGGCTGGCCTACGTCCTCCTGCTGCTCCTGGAGCTG CTGGTCTGCCTCTTCACCCTCCTGGGCCTGGCGAAGCAGAGCAAGTGGCTGGTGATCGTG ATGACAGTCATGAGTCTCCTGGTTCTCGTCCTGAGCTGGGGCTCCATGGGCCTGGAGGCA GCCACGGCCGTGGGCCTCAGTGACTTCTGCTCCAATCCAGACCCTTATGTTCTGAACCTG ACCCAGGAGGAGACAGGGCTCAGCTCAGACATCCTGAGCTATTATCTCCTCTGCAACCGG GCCGTCTCCAACCCCTTCCAACAGAGGCTGACTCTGTCCCAGCGAGCTCTGGCCAACATC CACTCCCAGCTGCTGGGCCTGGAGCGAGAAGCTGTGCCTCAGTTCCCTTCAGCGCAGAAG CCTCTGCTGTCCTTGGAGGAGACTCTGAATGTGACAGAAGGAAATTTCCACCAGTTGGTG GCACTGCTACACTGCCGCAGCCTGCACAAGGACTATGGTGCAGCCCTGCGGGGCCTGTGC GAAGACGCCCTGGAAGGCCTGCTCTTCCTGCTGCTCTTCTCCCTGCTGTCTGCAGGAGCG CTGGCCACTGCCCTCTGCAGCCTGCCCCGAGCCTGGGCCCTCTTCCCACCCAGTGACGAC TACGATGACACAGACGATGACGACCCTTTCAACCCTCAGCAGGAATCCAAGCGCTTTGTG CAGTGGCAGTCGTCTATCTGAGCCCCTCCTNCCNNGCTGACTGGAGCCTGGCTCCNCTCT TCGTTCCTTCCCCTGCTGCGNAGAGACCCCACTAACCAGCCTGCTGGCTCTGACACTACA C
Restriction Sites	Please inquire
ACCN	BC019358
Insert Size	1257 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC019358.1, AAH19358.1
RefSeq Size	1257 bp
RefSeq ORF	717 bp
Locus ID	57348
Protein Families	Ion Channels: Other, Transmembrane
Gene Summary	This gene encodes a member of the tweety family of proteins. Members of this family function as chloride anion channels. The encoded protein functions as a calcium(2+)-independent, volume-sensitive large conductance chloride(-) channel. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jan 2011]

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