Cezanne (OTUD7B) (BC020622) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	cellular zinc finger anti-NF-kappaB Cezanne; CEZANNE; OTTHUMP00000014192; OTU domain containing 7B; ZA20D1; zinc finger, A20 domain containing 1; zinc finger protein Cezanne
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC020622 edited GGCAAAAAGCCACTGGGGGAGGCCAGTCCCGATCAGTCCTTTCTGGCTCCAGCTCCAGAT CCCGTGCGGAGCAACAGCTGCGGCCTGAATTTTGATCTATGTTCTACCTACATCCAGCTG GCTGAAAGTACTTGAGGATCACATGACCCTGGACATGGATGCTGTTCTGTCAGATTTTGT CCGTTCCACAGGAGCAGAGCCAGGGCTAGCGCGAGATCTCCTAGAAGGAAAGAATTGGGA TGTGAATGCCGCCCTCAGTGATTTTGAACAGCTACGTCAAGTCCATGCTGGAAACCTACC CCCATCCTTTAGTGAGGGGAGTGGTGGCTCCAGGACCCCTGAAAAAGGGTTTTCTGACAG AGAGCCTACTCGCCCTCCCCGACCCATCCTCCAGCGGCAGGATGACATCGTTCAAGAAAA ACGCCTGTCTAGGGGCATCTCCCACGCCAGCTCCAGCATTGTTTCCCTGGCCCGGTCCCA TGTCTCCTCCAATGGTGGGGGTGGGGGGAGCAATGAGCACCCCCTGGAAATGCCCATCTG TGCCTTCCAGCTTCCAGATCTCACTGTATACAATGAAGACTTCCGCAGCTTCATAGAGAG AGACCTCATTGAGCAGTCCATGCTGGTTGCCTTGGAACAGGCAGGGCGTTTGAACTGGTG GGTGAGTGTGGATCCCACCTCTCAGAGGCTGCTTCCTTTGGCAACTACTGGAGATGGGAA CTGCCTCCTGCATGCAGCCTCCCTTGGAATGTGGGGTTTCCATGATCGGGACTTGATGCT GCGGAAAGCTTTGTATGCACTGATGGAGAAGGGAGTTGAGAAGGAAGCGTTGAAAAGGCG CTGGAGGTGGCAGCAGACACAGCAGAATGAAGAGTCAGGGCTGGTATACACAGAAGATGA ATGGCAGAAGGAGTGGAATGAACTGATCAAGCTTGCCTCAAGTGAACCCCGAATGCATCT AGGTACCAATGGAGCCAACTGTGGTGGGGTGGAGAGTTCTGAGGAGCCTGTATATGAGAG CCTTGAAGAGTTTCACGTCTTTGTCCTTGCTCATGTGCTTAGGAGGCCCATAGTCGTCGT GGCAGACACCATGCTGAGGGACTCCGGAGGGGAAGCATTTGCCCCTATTCCCTTTGGAGG AATCTATCTGCCTTTGGAGGTCCCAGCCAGCCAGTGTCACCGCTCCCCTCTGGTGCTCGC CTATGATCAGGCCCACTTTTCTGCACTCGTGTCCATGGAGCAGAAGGAGAATACCAAGGA ACAAGCTGTGATCCCACTTACAGATTCAGAGTATAAGCTGCTGCCCTTGCACTTTGCTGT GGACCCTGGAAAGGGCTGGGAGTGGGGCAAAGATGATAGTGACAATGTCCGATTGGCCAG TGTAATTCTGTCCCTAGAGGTCAGATTGCATCTGCTGCATAGCTAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC020622 unedited GGGGACTAGGATTTGTAATACGACTTACTATAGGCGGCCGCGACATTCGCCATTACGGCC GGGGGGCAAAAAGCCACTGGGGGAGGCCAGTCCCGATCAGTCCTTTCTGGCTCCAGCTCC AGATCCCGTGCGGAGCAACAGCTGCGGCCTGAATTTTGATCTATGTTCTACCTACATCCA GCTGGCTGAAAGTACTTGAGGATCACATGACCCTGGACATGGATGCTGTTCTGTCAGATT TTGTCCGTTCCACAGGAGCAGAGCCAGGGCTAGCGCGAGATCTCCTAGAAGGAAAGAATT GGGATGTGAATGCCGCCCTCAGTGATTTTGAACAGCTACGTCAAGTCCATGCTGGAAACC TACCCCCATCCTTTAGTGAGGGGAGTGGTGGCTCCAGGACCCCTGAAAAAGGGTTTTCTG ACAGAGAGCCTACTCGCCCTCCCCGACCCATCCTCCAGCGGCAGGATGACATCGTTCAAG AAAAACGCCTGTCTAGGGGCATCTCCCACGCCAGCTCCAGCATTGTTTCCCTGGCCCGGT CCCATGTCTCCTCCAATGGTGGGGGTGGGGGGAGCAATGAGCACCCCCTGGAAATGCNCA TCTGTGCCTTCCAGCTTCCAGATCTCACTGTATACAATGAAGACTTCCGCAGCTTCATAG AGAGAGACCTCATTGAGCAGTCCATGCTGGTTGCCTTGGAAACAGCAGGNCGTTTGAACT GGTGGGTGAGTGTGGATCCCACCTCTCAGAGGCTGCTTCCTTTGGCAACTACTGGAGATG GGAACTGCCTNCTGCATGCAGCCTCCCTTGGAAATGTGGGGTTTCCATGATCCGGACTTG ATGCTGCGGGAAGCTTTGTATGCACTGATGGAGAAGGAGTTGAGAAGAAGCGTTGAAAAG CGCTGGNAGTGGCA
Restriction Sites	Please inquire
ACCN	BC020622
Insert Size	1454 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC020622.1, AAH20622.1
RefSeq Size	1454 bp
Locus ID	56957
Gene Summary	Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections (By similarity). Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241). Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2 (By similarity). Mediates deubiquitination of EGFR (PubMed:22179831). Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains (PubMed:27732584). Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro); however the physiological significance of these data are unsure (PubMed:27732584). Hydrolyzes both linear and branched forms of polyubiquitin.[UniProtKB/Swiss-Prot Function]

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