ZNFN1A2 (IKZF2) (BC028936) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HELIOS; IKAROS family zinc finger 2 (Helios); MGC34330; zinc finger DNA binding protein Helios; zinc finger protein, subfamily 1A, 2 (Helios); ZNF1A2; ZNFN1A2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC028936 edited AACCCTGCTCCTCGCTGAAGATGGAGGAAGTAAAAACAGGATTACCCTTAGCTACAGATC CACTGCCTTAGTTTCCACCACCAACTGCAGTGCACAAACACACGTTAGGCACAGGAAAGA AAGAAAGACAGAGGACACATTAACAGTAAACACAAACAAAAGGGTGATGGGATTATTTTA CTGCATGCACTGCTGAGCCCGACATTGTCACCTCCTCTTTGAGGGGTTAGAAGAAGCTGA GATCTCCCGACAGAGCTGGAAATGGTGATGAATCTTTTTTAATCAAAGGACAATTTCTTT TCATTGCACTTTGACTATGGAAACAGAGGCTATTGATGGCTATATAACGTGTGACAATGA GCTTTCACCCGAAAGGGAGCACTCCAATATGGCAATTGACCTCACCTCAAGCACACCCAA TGGACAGCATGCCTCACCAAGTCACATGACAAGCACAAATTCAGTAAAGCTAGAAATGCA GAGTGATGAAGAGTGTGACAGGAAACCCCTGAGCCGTGAAGATGAGATCAGGGGCCATGA TGAGGGTAGCAGCCTAGAAGAACCCCTAATTGAGAGCAGCGAGGTGGCTGACAACAGGAA AGTCCAGGAGCTTCAAGGCGAGGGAGGAATCCGGCTTCCGAATGGTGAACGCCCCTTCCA CTGTAACCAGTGTGGAGCTTCTTTTACTCAGAAGGGCAACCTTCTGAGACACATAAAGTT ACACTCTGGAGAGAAGCCGTTCAAATGTCCTTTCTGTAGCTACGCCTGTAGAAGAAGGGA CGCCCTCACAGGACACCTCAGGACCCATTCTGTGGGTAAACCTCACAAGTGCAACTACTG TGGACGAAGCTACAAGCAGCGCAGTTCACTGGAGGAGCACAAGGAACGCTGCCACAACTA TCTCCAGAATGTCAGCATGGAGGCTGCTGGGCAGGTCATGAGTCACCATGTACCTCCTAT GGAAGATTGTAAGGAACAAGAGCCTATTATGGACAACAATATTTCTCTGGTGCCTTTTGA GAGACCTGCTGTCATAGAGAAGCTCACGGGGAATATGGGAAAACGTAAAAGCTCCACTCC ACAAAAGTTTGTGGGGGAAAAGCTCATGCGATTCAGCTACCCAGATATTCACTTTGATAT GAACTTAACATATGAGAAGGAGGCTGAGCTGATGCAGTCTCATATGATGGACCAAGCCAT CAACAATGCAATCACCTACCTTGGAGCTGAGGCCCTTCACCCTCTGATGCAGCACCCGCC AAGCACAATCGCTGAAGTGGCCCCAGTTATAAGCTCAGCTTATTCTCAGGTCTATCATCC AAATAGGATAGAAAGACCCATTAGCAGGGAAACTGCTGATAGTCATGAAAACAACATGGA TGGCCCCATCTCTCTCATCAGACCAAAGAGTCGACCCCAGGAAAGAGAGGCCTCTCCCAG CAATAGCTGCCTGGATTCCACTGACTCAGAAAGCAGCCATGATGACCACCAGTCCTACCA AGGACACCCTGCCTTAAATCCCAAGAGGAAACAAAGCCCAGCTTACATGAAGGAGGATGT CAAAGCTTTGGATACTACCAAGGCTCCTAAGGGCTCTCTGAAGGACATCTACAAGGTCTT CAATGGAGAAGGAGAACAGATTAGGGCCTTCAAGTGTGAGCACTGCCGAGTCCTTTTCCT AGACCATGTCATGTACACCATTCACATGGGTTGCCATGGCTACCGGGACCCACTGGAATG CAACATCTGTGGCTACAGAAGCCAGGACCGTTATGAGTTTTCATCACACATTGTTCGAGG GGAGCACACATTCCACTAGGCCTTTTCATTCCAAAGGGGACCCCTATGAAGTAAAGAACT GCACATGAAGAAATACTGCACTTACAATCCCACCTTTCCTCAAATGTTGACATACCTTTT ATTTTTTTTAATATTATTACTGTTGATAATTCTTATTTTGTGGAGGCAGTGTCATTTGCT CTGCCTAATTACGATAAGGAAGAAACAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC028936 unedited NNNNCCCGGTTCGNGATTTTGTATACGACTCATATAGGCGGCCGCGNATTCCCGGGNTAT CGTCGACCCAGCGTCCGAACCCTGCTCCTCGCTGAAGATGGAGAAGTAAAAACAGGATTA CCCTTAGCTACAGATCCACTGCCTTAGTTTCCACCACCAACTGCAGTGCACAAACACACG TTAGGCACAGGAAAGAAAGAAAGACAGAGGACACATTAACAGTAAACACAAACAAAAGGG TGATGGGATTATTTTACTGCATGCACTGCTGAGCCCGACATTGTCACCTCCTCTTTGAGG GGTTAGAAGAAGCTGAGATCTCCCGACAGAGCTGGAAATGGTGATGAATCTTTTTTAATC AAAGGACAATTTCTTTTCATTGCACTTTGACTATGGAAACAGAGGCTATTGATGGCTATA TAACGTGTGACAATGAGCTTTCACCCGAAAGGGAGCACTCCAATATGGCAATTGACCTCA CCTCAAGCACACCCAATGGACAGCATGCCTCACCAAGTCACATGACAAGCACAAATTCAG TAAAGCTAGAAATGCAGAGTGATGAAGAGTGTGACAGGAAACCCCTGAGCCGTGAAGATG AGATCAGGGGCCATGATGAGGGTAGCAGCCTAGAAGAACCCCTAATTGAGAGCAGCGAGG TGGCTGACAACAGGAAAGTCCAGGAGCTTCAAGGCGAGGGAGGAATCCGGCTTCCGAATG GTGAACGCCCCTTCCACTGTNACCAGTGTGGAGCTTCTTTTACTCAGAAAGGCAACCTTC TGAGACACATAAAGTTACACTCTGGAGAGAAGCCCGTTCAATGTCCTTTTCTGTAGCTAC GCCTGTAGAAAGAAGGACGCCCTCACAGGACACCTCANGACCCCATTCTGTGGTAAACA
Restriction Sites	Please inquire
ACCN	BC028936
Insert Size	2018 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC028936.1, AAH28936.1
RefSeq Size	2018 bp
Locus ID	22807
Gene Summary	This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

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