MRPL2 (BC020212) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CGI-22; mitochondrial ribosomal protein L2; MRP-L14; OTTHUMP00000016419; RPML14
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC020212 edited GTCGTGCTGATGGCCCTGTGCGCACTGACCCGCGCTCTGCGCTCTCTGAACCTGGCGCCC CCGACCGTCGCCGCCCCTGCCCCGAGTCTGTTCCCCGCCGCCCAGATGATGAACAATGGC CTCCTCCAACAGCCCTCTGCCTTGATGTTGCTCCCCTGCCGCCCAGTTCTTACTTCTGTG GCCCTTAATGCCAACTTTGTGTCCTGGAAGAGTCGTACCAAGTACACCATTACACCAGTG AAGATGAGGAAGTCTGGGGGCCGAGACCACACAGGCCGAATCCGGGTGCATGGTATTGGC GGGGGCCACAAGCAACGTTATCGAATGATTGACTTTCTGCGTTTCCGGCCTGAGGAGACC AAGTCAGGACCCTTTGAGGAGAAGGTTATCCAAGTCCGCTATGATCCCTGTAGGTCAGCA GACATAGCTCTGGTTGCTGGGGGCAGCCGGAAACGCTGGATCATCGCCACAGAAAACATG CAGGCTGGAGATACAATCTTGAACTCTAACCACATAGGCCGAATGGCAGTTGCTGCTCGG GAAGGGGATGCGCATCCTCTTGGGGCTCTGCCTGTGGGGACCCTCATCAACAACGTGGAA AGTGAGCCAGGCCGGGGTGCCCAATATATCCGAGCTGCAGGGACGTGTGGTGTGCTACTG CGGAAGGTGAATGGCACAGCCATTATCCAGCTGCCCTCTAAGAGGCAGATGCAGGTGCTG GAAACGTGCGTAGCAACAGTAGGCCGAGTATCCAACGTTGATCATAACAAACGGGTCATT GGCAAGGCAGGTCGCAACCGCTGGCTGGGCAAGAGGCCTAACAGTGGGCGGTGGCACCGC AAGGGGGGCTGGGCTGGCCGAAAGATTCGGCCACTACCCCCCATGAAGAGTTACGTGAAG CTGCCTTCTGCTTCTGCCCAAAGCTGATATCCCTGTACTCTAATAAAATGCCCCCCCCCC CCCGTTTTAATCTGTAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC020212 unedited NNGGAGTTCACAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACCGGGTCG TGCTGATGGCCCTGTGCGCACTGACCCGCGCTCTGCGCTCTCTGAACCTGGCGCCCCCGA CCGTCGCCGCCCCTGCCCCGAGTCTGTTCCCCGCCGCCCAGATGATGAACAATGGCCTCC TCCAACAGCCCTCTGCCTTGATGTTGCTCCCCTGCCGCCCAGTTCTTACTTCTGTGGCCC TTAATGCCAACTTTGTGTCCTGGAAGAGTCGTACCAAGTACACCATTACACCAGTGAAGA TGAGGAAGTCTGGGGGCCGAGACCACACAGGCCGAATCCGGGTGCATGGTATTGGCGGGG GCCACAAGCAACGTTATCGAATGATTGACTTTCTGCGTTTCCGGCCTGAGGAGACCAAGT CAGGACCCTTTGAGGAGAAGGTTATCCAAGTCCGCTATGATCCCTGTAGGTCAGCAGACA TAGCTCTGGTTGCTGGGGGCAGCCGGAAACGCTGGATCATCGCCACAGAAAACATGCAGG CTGGAGATACAATCTTGAACTCTAACCACATAGGCCGAATGGCAGTTGCTGCTCGGGAAG GGGATGCGCATCCTCTTGGGGCTCTGCCTGTGGGGACCCTCATCAACAACGTGGAAAGTG AGCCAGGCCGGGGTGCCCAATATATCCGAGCTGCAGGGACGTGTGGTGTGCTACTGCGGA AGGTGAATGGCACAGCCATTATCCAGCTGCCCTCTAAGAGGCAGATGCAGGTGCTGGAAA CGTGCGTAGCAACAGTAGGCCGAGTATCCAACGTTGATCATAACANACGGGTCATTGGCA AGGNCAGTCGCAACCGCTGGCTGGGCAAGATGCCCTACAGTGGGGCGGTGGCACCGCAG
Restriction Sites	Please inquire
ACCN	BC020212
Insert Size	993 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC020212.1, AAH20212.1
RefSeq Size	993 bp
Locus ID	51069
Gene Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]

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