BRDG 1 (STAP1) (BC014958) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BCR downstream signaling 1; BRDG1; OTTHUMP00000159640; signal-transducing adaptor protein-1; signal transducing adaptor family member 1; STAP-1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC014958 edited GAGAAACCAAACCACACACCAAAGAGAGGGGTATGATGGCTAAGAAGCCCCCAAAACCAG CCCCTCGCAGGATCTTCCAGGAAAGGTTAAAGATTACTGCTCTACCTTTGTACTTTGAAG GTTTTTTATTAATCAAGCGGTCAGGATACCGGGAGTATGAGCATTACTGGACAGAGTTGA GAGGAACTACTCTTTTCTTTTATACCGACAAAAAGAGTATAATATATGTTGACAAATTAG ACATAGTAGACCTCACATGCCTTACTGAGCAGAATTCAACTGAAAAGAACTGTGCGAAAT TCACCCTTGTTTTGCCGAAAGAGGAAGTACAACTGAAGACAGAGAACACAGAAAGTGGGG AAGAATGGAGAGGCTTCATTCTTACAGTAACAGAGCTGTCAGTTCCCCAAAACGTGTCAC TCCTACCTGGGCAAGTAATTAAACTGCATGAAGTCCTAGAGAGAGAAAAGAAAAGGAGGA TTGAGACAGAGCAGAGTACGTCCGTGGAAAAAGAGAAGGAACCAACTGAAGATTATGTGG ATGTACTGAACCCTATGCCAGCATGTTTTTATACAGTGTCCCGGAAAGAGGCAACTGAGA TGCTCCAGAAGAACCCTTCTTTGGGAAATATGATCCTGAGGCCTGGTAGTGACAGTAGAA ACTACTCCATCACTATTCGGCAGGAGATAGACATTCCAAGAATCAAGCACTACAAAGTGA TGAGCGTAGGACAAAACTACACTATTGAACTGGAAAAACCTGTAACACTCCCAAACCTTT TCAGTGTCATTGATTATTTTGTGAAGGAGACTCGAGGAAATTTAAGACCATTTATATGTT CAACTGATGAAAACACTGGTCAAGAACCCAGTATGGAAGGGAGAAGTGAAAAGTTGAAGA AAAATCCACACATTGCATGAAATACAATGTGAAAGCTCCTTTGTATATCTTGGGGAAAGA CTATGTGAGACAGAGGGAGAAAACAGCCATTGACAACTCAAGGAGAGAGGCCTCGGGGGG AAAAAAAGATCAACCTGGCCAAAACCTTGAAAATCCGGCTTCTTGACTTGTGAGAAAATA AATTTGTGTTCCTTAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC014958 unedited CCATATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGAAGAAAC CAAACCACACACCAAAGAGAGGGGTATGATGGCTAAGAAGCCCCCAAAACCAGCCCCTCG CAGGATCTTCCAGGAAAGGTTAAAGATTACTGCTCTACCTTTGTACTTTGAAGGTTTTTT ATTAATCAAGCGGTCAGGATACCGGGAGTATGAGCATTACTGGACAGAGTTGAGAGGAAC TACTCTTTTCTTTTATACCGACAAAAAGAGTATAATATATGTTGACAAATTAGACATAGT AGACCTCACATGCCTTACTGAGCAGAATTCAACTGAAAAGAACTGTGCGAAATTCACCCT TGTTTTGCCGAAAGAGGAAGTACAACTGAAGACAGAGAACACAGAAAGTGGGGAAGAATG GAGAGGCTTCATTCTTACAGTAACAGAGCTGTCAGTTCCCCAAAACGTGTCACTCCTACC TGGGCAAGTAATTAAACTGCATGAAGTCCTAGAGAGAGAAAAGAAAAGGAGGATTGAGAC AGAGCAGAGTACGTCCGTGGAAAAAGAGAAGGAACCAACTGAAGATTATGTGGGATGTAC TGAACCCTATGCCAGCATGTTTTTATACAGTGTCCCGGAAAAGAGCAACTGAGATGCTCC AGAAGAACCCTTTCTTTGGAAATATGATCCCTGAGCCTGGTAGTGACAGTAGAAACTACT CCATCACTATTCGGGCAGAGATAGCCTTTCCAAGATCAAGCCTACCAATTGATGAGCGTA GGACAAACTACACTATTGAACTGGGAAAAACTGTAACACTCCCAAACTTTTCGTGTCATT GATTATTTTGGGGAGGAAACTCGGGGAAATTTAAGACCTTTAATTGTCAACTGATGAAAC CCTGGTCAAGACCCGTTTGGAGGGAAAATTGAAATTTTGAAAAA
Restriction Sites	Please inquire
ACCN	BC014958
Insert Size	1114 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC014958.1, AAH14958.1
RefSeq Size	1114 bp
Locus ID	26228
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene contains a proline-rich region, a pleckstrin homology (PH) domain, and a region in the carboxy terminal half with similarity to the Src Homology 2 (SH2) domain. This protein is a substrate of tyrosine-protein kinase Tec, and its interaction with tyrosine-protein kinase Tec is phosphorylation-dependent. This protein is thought to participate in a positive feedback loop by upregulating the activity of tyrosine-protein kinase Tec. Variants of this gene have been associated with autosomal-dominant hypercholesterolemia (ADH), which is characterized by elevated low-density lipoprotein cholesterol levels and in increased risk of coronary vascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

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