CEP250 (BC001433) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C-NAP1; CEP2; CNAP1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC001433 edited GGCACGAGGCAGGCTGGGAGGGTAGAGTTGTTGGCAGAAATCCTGGGATAAGAGAATAGT TTCCTGGAAGATCTGTGCCTCCAACCAGCAGAGAGGGATTGAGCTTCATTGAACTCAACA GAGCCAACATTTCATAGCACCATGTTCAAGAGGAGGTTGAAGTGGCATGGCAATGGTTAG AGACCCTGCTGGGCGTGAACACCCTCTGGCTACCTAGGGACCTGTGGGCCTACCACCTGG TGCCCTCATGGAGACAAGAAGCCCTGGGTTGAACAACATGAAGCCCCAGTCACTGCAGCT GGTACTGGAAGAGCAGGTGCTGGCACTACAGCAGCAGATGGCAGAGAATCAGGCAGCCTC CTGGCGGAAGCTGAAGAACTCCCAGGAGGCCCAGCAGAGACAAGCAACCCTTGTGAGGAA GCTGCAGGCCAAGGTGCTGCAGTACCGAAGCTGGTGCCAAGAGCTGGAGAAGCGGCTAGA AGCCACTGGAGGACCAATCCCCCAGAGGTGGGAAAATGTGGAGGAGCCAAACCTGGATGA GCTGCTGGTCCGATTGGAGGAGGAGCAACAGAGGTGTGAGAGTCTAGCAGAGGTGAACAC CCAGCTTCGACTGCACATGGAAAAAGCTGACGTGGTGAATAAAGCCCTTAGGGAAGATGT GGAAAAACTGACAGTGGACTGGAGCCGGGCCCGGGATGAGCTAATGAGGAAGGAGAGCCA GTGGCAGATGGAGCAGGAGTTCTTCAAGGGCTACCTGAAAGGGGAGCACGGTCGCCTTCT CAGTCTATGGCGGGAGGTTGTGACATTCCGACGCCACTTCCTGGAAATGAAGTCAGCTAC TGACAGAGATCTGATGGAGCTAAAAGCTGAGCATGTGAGGCTTTCAGGGTCTCTGTTGAC CTGTTGTCTGCGCTTGACTGTGGGAGCACAGTCTCGGGAACCCAACGGATCTGGAAGAAT GGATGGGCGGGAGCCGGCCCAGCTGCTGCTGCTACTAGCCAAGACCCAGGAGCTGGAGAA GGAAGCCCATGAAAGGAGCCAGGAGTTAATACAGCTGAAGAGTCAAGGGGATCTGGAGAA GGCTGAACTTCAGGACCGGGTGACCGAGCTCTCTGCTCTGTTGACCCAGTCTCAGAAGCA AAATGAAGATTATGAAAAGATGATAAAGGCTCTGAGAGAGACATTTTAAATCCTGAAGAA ATTGAGAAGTATGTTGCTGAAATTGAAAAAGAAAAAGAAGAAAACGAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC001433 unedited AAATTGTCAGATTTGTATACGACTCACTATAGGCGGCACGCGAATTCGCACGAGGCAGGC TGGGAGGGTAGAGTTGTTGGCAGAAATCCTGGGATAAGAGAATAGTTTCCTGGAAGATCT GTGCCTCCAACCAGCAGAGAGGGATTGAGCTTCATTGAACTCAACAGAGCCAACATTTCA TAGCACCATGTTCAAGAGGAGGTTGAAGTGGCATGGCAATGGTTAGAGACCCTGCTGGGC GTGAACACCCTCTGGCTACCTAGGGACCTGTGGGCCTACCACCTGGTGCCCTCATGGAGA CAAGAAGCCCTGGGTTGAACAACATGAAGCCCCAGTCACTGCAGCTGGTACTGGAAGAGC AGGTGCTGGCACTACAGCAGCAGATGGCAGAGAATCAGGCAGCCTCCTGGCGGAAGCTGA AGAACTCCCAGGAGGCCCAGCAGAGACAAGCAACCCTTGTGAGGAAGCTGCAGGCCAAGG TGCTGCAGTACCGAAGCTGGTGCCAAGAGCTGGAGAAGCGGCTAGAAGCCACTGGAGGAC CAATCCCCCAGAGGTGGGAAAATGTGGAGGAGCCAAACCTGGATGAGCTGCTGGTCCGAT TGGAGGAGGAGCAACAGAGGTGTGAGAGTCTAGCAGAGGTGAACACCCAGCTTCGACTGC ACATGGAAAAAGCTGACGTGGTGAATAAAGCCCTTAGGGAAGATGTGGAAAAACTGACAG TGGACTGGAGCCGGGCCCGGGATGAGCTAATGAGGAAGGAGAGCCAGTGGCAGATGGAGC ANGAGTTCTTCAAGGCTACCTGAAAGGGGAGCACGGTCGCCTTCTCAGTCTATGCNGGGA GGTTGTGACATTCCGACGCCACTTCCTGGAAATGAAGTCAGCTACGGACAGAGT
Restriction Sites	Please inquire
ACCN	BC001433
Insert Size	1286 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC001433.1, AAH01433.1
RefSeq Size	1286 bp
Locus ID	11190
Gene Summary	This gene encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The encoded protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

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