POLE (BC021559) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DKFZp434F222; DNA polymerase epsilon catalytic subunit; FLJ21434; POLE1; polymerase (DNA directed), epsilon
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC021559 edited CTCTCTGCTTCATGACCCTGCCCTGCACCGCACACTCCACAACATGATGAAGAAGCTCTT CCTGCAGCTCATCGCTGAGTTCAAGCGCCTGGGGTCATCAGTCATCTACGCCAACTTCAA CCGCATCATCCTCTGTACAAAGAAGCGCCGTGTGGAAGATGCCATCGCTTACGTGGAGTA CATCACCAGCAGCATCCATTCAAAGGAGACCTTCCATTCTCTGACAATTTCTTTCTCTCG ATGCTGGGAATTTCTTCTCTGGATGGATCCATCTAACTATGGCGGAATCAAAGGAAAAGT TTCATCTCGTATTCACTGTGGACTGCAAGACTCCCAGAAAGCAGGGGGAGCAGAGGATGA GCAGGAAAATGAGGACGATGAGGAGGAAAGAGATGGGGAGGAGGAGGAAGAGGCGGAGGA ATCCAACGTGGAGGATTTACTGGAAAACAACTGGAACATTTTGCAGTTTTTGCCACAGGC AGCCTCCTGCCAGAACTACTTCCTCATGATTGTTTCAGCGTACATCGTGGCCGTGTACCA CTGCATGAAGGACGGGCTGAGGCGCAGTGCTCCAGGGAGCACCCCCGTGAGGAGGAGGGG GGCCAGCCAGCTCTCCCAGGAGGCCGAGGGGGCGGTCGGAGCCCTTCCCGGAATGATCAC CTTCTCTCAGGATTATGTCGCAAATGAGCTCACTCAGAGCTTCTTCACCATCACTCAGAA GATTCAGAAGAAAGTCACAGGCTCTCGGAACTCCACTGAGCTCTCAGAGATGTTTCCTGT CCTCCCCGGTTCCCACTTGCTGCTCAATAACCCTGCCCTGGAGTTCATCAAATACGTGTG CAAGGTGCTGTCCCTGGACACCAACATCACAAACCAGGTGAATAAGCTGAACCGAGACCT GCTTCGCCTGGTGGATGTCGGCGAGTTCTCCGAGGAGGCCCAGTTCCGAGACCCCTGCCG CTCCTACGTGCTTCCTGAGGTCATCTGCCGCAGCTGTAACTTCTGCCGCGACCTGGACCT GTGTAAAGACTCTTCCTTCTCAGAGGATGGGGCGGTCCTGCCTCAGTGGCTCTGCTCCAA CTGTCAGGCGCCCTACGACTCCTCTGCCATCGAGATGACGCTGGTGGAAGTTCTACAGAA GAAGCTGATGGCCTTCACCCTGCAGGACCTGGTCTGCCTGAAGTGCCGCGGGGTGAAGGA GACCAGCATGCCTGTGTACTGCAGCTGCGCGGGAGACTTCGCCCTCACCATCCACACCCA GGTCTTCATGGAACAGATCGGAATATTCCGGAACATTGCCCAGCACTACGGCATGTCGTA CCTCCTGGAGACCCTGGAGTGGCTGCTGCAGAAGAACCCACAGCTGGGCCATTAGCCAGC CCCGGGCCCCGGGTGCCTCTGCGTCCGTGCCAGGCCTCCTGATGCCAAGGCCACATCCCC GTGCTTCCAGTGACCAGACCACTGACCACCCTGACTGTCCAAACCTGTGACCCCAGGCCA GGGAACGGGGAGGAAACCAAAGAAAACCATTTTCAGGGAGCTCAGACGTCACAGGAGGGA GCGGGAGCAGGATGTGGCCCTGGCCTCGCCAGAGCACCTGAAGAAGCAGGCCGTGAGCGA GGCTGCGAGTGCCCTGGGCGCCGTTTCTCACGCAGTGAATGCTTTTCCAGGCCTCTGTTG CTTCCTGCACCACACCTGGTGGGGTGGGAGCGTCCTCTAGGTGCCCCTAGTTCTTTGTCC TGCCTCCCAGAGGGAGGAAAAGCCCCTGGGGGCTTCTGGCTCCCTGAGATTGGGCTCTGA GACGAGACGGGTTCCCAAGGCCCTGGTGGGGCTGGAGTCTCACCTGTTTGCATGGAGAAA TGGGCTGGCCCCACAGCCTCACAGGAGCAGTTTGTGGGCTGGTTTCCCCAGGAATCCAGA CCCTAACCCGTGAGAATCTGGATTTTGGCTTGTGAGCCCTGCTTATTTGGAGCCGGGTCT AGAGGGAACCCTCTATCAGCCTCAGGAAAACAAGACCTCTGTGCACCTCACTTTTGGCTC ACTGCAGCCCTTGTCCTTCACCTCCACACAGGACCAGCTGGAAGCAGAAAGAAGAAAGGC CAATTTCACAGGGCACCAAACAAGTATGAAATGTAAATCAGAAATGCAGACACCCCAGAC GAGAGCCTCACAGGAGGGAGGGGGCCCCACAGGCTCCCCAGGAGGCTCGTGTCTTTGGCC CAGAGCCAGCCTTAGTTTGTCCCTGCCATCTACTGTCTGAGGCCATCGCTGCTACACTTT GTTTTTATTTGTATTTCATACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC021559 unedited GGGGTTCATATTTTGTAATACGACTCACTATAGGGCGGGCCGCGCAATTCGCACGAGGCT CTCTGCTTCATGACCCTGCCCTGCACCGCACACTCCACAACATGATGAAGAAGCTCTTCC TGCAGCTCATCGCTGAGTTCAAGCGCCTGGGGTCATCAGTCATCTACGCCAACTTCAACC GCATCATCCTCTGTACAAAGAAGCGCCGTGTGGAAGATGCCATCGCTTACGTGGAGTACA TCACCAGCAGCATCCATTCAAAGGAGACCTTCCATTCTCTGACAATTTCTTTCTCTCGAT GCTGGGAATTTCTTCTCTGGATGGATCCATCTAACTATGGCGGAATCAAAGGAAAAGTTT CATCTCGTATTCACTGTGGACTGCAAGACTCCCAGAAAGCAGGGGGAGCAGAGGATGAGC AGGAAAATGAGGACGATGAGGAGGAAAGAGATGGGGAGGAGGAGGAAGAGGCGGAGGAAT CCAACGTGGAGGATTTACTGGAAAACAACTGGAACATTTTGCAGTTTTTGCCACAGGCAG CCTCCTGCCAGAACTACTTCCTCATGATTGTTTCAGCGTACATCGTGGCCGTGTACCACT GCATGAAGGACGGGCTGAGGCGCAGTGCTCCAGGGAGCACCCCCGTGAGGAGGAGGGGGG CCAGCCAGCTCTCCCAGGAGGCCGAGGGGGCGGTCGGAGCCCTTCCCGGATGATCACCTT CTCTCAGGATTATGTCGCANATGAGCTCACTCAGAGCTTCTTCACCATCACTCAGAAGAT TCAGAAGAAAGTCACAGGCTCTCGGGACTCCACTGAGCTCTCAGAGATGTTTCCTGTCCT CCCGGTTCCCACTTGCTGCTCAATAACCCTGCCCTGGAGTTCATCAAATACGTGTGCAAG GTGCTGTCCA
Restriction Sites	Please inquire
ACCN	BC021559
Insert Size	2334 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021559.1, AAH21559.1
RefSeq Size	2334 bp
Locus ID	5426
Protein Families	Druggable Genome
Protein Pathways	Base excision repair, DNA replication, Metabolic pathways, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism
Gene Summary	This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.