JMJD7 (BC025290) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_001114632 edited CCATGGCGGAGGCGGCTTTGGAAGCCGTGCGGAGCGAGTTACGAGAATTCCCGGCCGCTG CAAGGGAGCTCTGCGTGCCTCTTGCTGTGCCCTACCTGGACAAACCCCCAACTCCGCTCC ACTTCTACCGGGACTGGGTCTGCCCCAACAGGCCGTGCATTATCCGCAACGCTCTGCAGC ACTGGCCGGCCCTCCAGAAGTGGTCCCTCCCCTATTTCAGAGCCACAGTGGGCTCCACAG AGGTGAGTGTGGCCGTGACCCCAGATGGTTACGCGGATGCCGTGAGAGGGGATCGCTTCA TGATGCCAGCTGAGCGCCGCCTGCCCCTGAGCTTCGTGCTGGATGTGCTGGAGGGCCGGG CCCAGCACCCTGGAGTCCTCTATGTGCAGAAGCAGTGCTCCAACCTGCCCAGCGAGCTGC CCCAGCTGCTGCCTGATCTGGAATCCCATGTGCCCTGGGCCTCCGAAGCCCTGGGAAAGA TGCCCGATGCTGTGAACTTCTGGCTGGGGGAGGCGGCTGCAGTGACTTCTTTGCACAAGG ACCACTATGAGAACCTCTACTGCGTGGTCTCAGGAGAGAAGCATTTCCTGTTCCATCCGC CCAGCGACCGGCCCTTCATCCCCTATGAGCTGTACACGCCGGCAACCTACCAGCTAACTG AAGAGGGCACCTTTAAGGTGGTGGATGAAGAGGCCATGGAGAAGGTGCCCTGGATCCCAC TGGACCCCTTGGCGCCAGACCTAGCACGGTACCCTAGTTACAGTCAGGCCCAGGCCCTTC GCTGCACGGTGCGGGCCGGTGAGATGCTCTATCTGCCGGCTCTGTGGTTCCACCACGTCC AGCAGTCCCAGGGCTGCATCGCAGTGAATTTCTGGTATGACATGGAATACGACCTCAAGT ATAGTTACTTCCAGCTGCTCGACTCCCTCACCAAGGCTTCAGGCCTTGACTGATGGAGCA CTGGTGAACACGACCAAGCACGCCTCGGGGGACGGAGCCAGCCCCTCCCTGGCCAGGTCA ATTCTCGAGAGAGCCTGGAGTGTGCATGCTGGCTGCTGGCCCCGGGTCCAGCATGGCTTG AGATCAGCTTTGGAGGATCTTGGAATGTGGTCATAAGGACTCAAGGTGCCAGGCAGGTCT GGGTGAGGGTTCTCAGGAAGTTGCCACACAGGTGAGCAGAGTGGGGATCAGGTGCAGCGG CACCTCTCCCCAGCGCTGTGATGTTGGGCGAGTCACTGCGTCTCGGGCATTGGTGTCCTG TCAGTAAAGAGATAATAATGGCTGTACCTCGCGGGGCTGTTGTGGGCTTGGAGATGATGT CTATGAGGACCAGCATGGAGCTGGCACACAGGACATGTTGAATAAAAGGTAGCTGTGAAA AAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC025290 unedited NAAAGTTCAAATTTTGTAATACGAACTCACTATAGGGCCGGCCGCGAATTCGCACGAGGC CATGGCGGAGGCGGCTTTGGAAGCCGTGCGGAGCGAGTTACGAGAATTCCCGGCCGCTGC AAGGGAGCTCTGCGTGCCTCTTGCTGTGCCCTACCTGGACAAACCCCCAACTCCGCTCCA CTTCTACCGGGACTGGGTCTGCCCCAACAGGCCGTGCATTATCCGCAACGCTCTGCAGCA CTGGCCGGCCCTCCAGAAGTGGTCCCTCCCCTATTTCAGAGCCACAGTGGGCTCCACAGA GGTGAGTGTGGCCGTGACCCCAGATGGTTACGCGGATGCCGTGAGAGGGGATCGCTTCAT GATGCCAGCTGAGCGCCGCCTGCCCCTGAGCTTCGTGCTGGATGTGCTGGAGGGCCGGGC CCAGCACCCTGGAGTCCTCTATGTGCAGAAGCAGTGCTCCAACCTGCCCAGCGAGCTGCC CCAGCTGCTGCCTGATCTGGAATCCCATGTGCCCTGGGCCTCCGAAGCCCTGGGAAAGAT GCCCGATGCTGTGAACTTCTGGCTGGGGGAGGCGGCTGCAGTGACTTCTTTGCACAAGGA CCACTATGAGAACCTCTACTGCGTGGTCTCAGGAGAGAAGCATTTCCTGTTCCATCCGCC CAGCGACCGGCCCTTCATCCCCTATGAGCTGTACACGCCGGCAACCTACCAGCTAACTGA AGAGGGCACCTTTAAGGTGGTGGATGAAGAGGCCATGGAGAAAGTGCCCTGNATCCCACT GGACCCCTTGGCGCCAGACCTAGCACGGTACCCTAGTTACAGTCAGGGCCCAGGCCCTTC GCTGCACGGTGCGGGCCGGTGAGATGCTCTATCTGCCGGCTCTGTGGTTCCACCACGTCC AGCAGTCCCAGGG
Restriction Sites	Please inquire
ACCN	BC025290
Insert Size	1400 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC025290.1, AAH25290.1
RefSeq Size	1395 bp
Locus ID	100137047
Gene Summary	This gene encodes a highly conserved protein with a JmjC domain, which are part of the cupin metalloenzyme superfamily. JmjC proteins may function as 2-oxoglutarate-Fe(II)-dependent dioxygenases. Most tissues also express read-through transcripts from this gene into the downstream phospholipase A2, group IVB (cytosolic) gene, some of which may encode fusion proteins combining the N-terminus of this protein with the phospholipase A2, group IVB protein. [provided by RefSeq, Jul 2008]

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