H3FH (HIST1H3G) (BC012185) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	H3/h; H3FH
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC012185 edited GCCTTATAATGGTCAAAGGTTTCCAGCTAGCAGCAGTCCAGGTGACTCAAGAAAACGGCA CAAGATAAACTTCTCAAAACGTGGTCCTCCGAATCTGGCCCCACAACACATCTACTGAAT GAGAATTAGCGTCTTCCAAGTCTTAAAGTAGTCGTTTACGGACTACAGCTTGAGAAGCGC AGAATTAGAACTTTAAGAAGTTAACGTTCTTGGATCGCAGACTAACCGAGAAGAGAGGAT TCAAAAATGAAGTGATTCATCCAATCAGATTTTCCAGTAATTCACCAATCACATCGTTGG ACTCTTCAGCCAATGGTTTTGTTGCGCGGGTCTTTTCAAAATCAACAAGGCCAATTAGAA TGTTTCTGAGTATATATAAGGGCAGTTTCCACTCTGCCAAGTGGCTGAGTTTTTCTTTCA GGTAGTCTGAGATGGCCCGCACCAAGCAGACTGCACGCAAGTCCACCGGTGGCAAAGCGC CGCGCAAGCAGCTGGCCACTAAGGCGGCTCGGAAAAGCGCGCCGGCCACCGGCGGCGTGA AGAAACCTCATCGCTACCGTCCCGGCACCGTGGCTCTGCGCGAGATTCGCCGCTATCAGA AGTCGACTGAGCTGCTGATCCGCAAGTTGCCTTTCCAACGCCTGGTGCGAGAAATCGCTC AGGACTTCAAGACAGATCTGCGCTTTCAGAGTTCCGCGGTGATGGCCCTGCAGGAGGCCT GCGAGGCCTACTTGGTGGGGCTCTTTGAGGATACCAACCTGTGTGCCATCCATGCTAAGC GAGTGACTATCATGCCCAAGGACATTCAGCTCGCTCGCCGCATTCGTGGGGAGAGAGCGT AGAGGTTTCCGGGCAGTAATTCTATCCAACTTAAAAAGGCTCTTTTAAGAGCCGCCTACT TTCACCCGAAAATAGCTGTGATTAACTAATCAAATCTTTGAAACTAAACATTGGTTTTAG TACTTAAGTCCTCATTTGATTGGCTAAAGTTAATGGTAAGCTTGCTATATTTGTTCCCGT GCTTTTTCCCGAAAGTGCCAGATTGTCAGCGTCCTTTTCTAAACTGGCTTTTAGGGGTTT GGAGCTTCGATTTGGTAGTGGAAGGGGCCACCTGGGGGCGTTTATATACATTGTCTCAAG CGTTAGTCGCGTTGTCTACTTACACCCATTATAGATGGGATTTTTTTTCGTTGTGTATTT CTGGTTAATCCCTTTTAAAACTTTACAGGTATGAGCATTGGCAGTTGAGAGTAGGTAGGG ATATCCTTGGTTGCTGCTTTTTCGTGTCTGAAGCAGCTATTTCCACAATTCCCCCTTTTT TTAAACAGTTCTTGGTTTTTCTGTGAAATGATCCATTTTTTAATTCAATATTAGAAATTG GCGGCAGAAACGAAAGCCAGATTGGCCTCTACATTACTGGCAGCATGGGCATTACGTGGG AACTTGTTAGAAGCTCCCAGGGCATTACATGGGAGCTTGTTTCAATCCCCATCTAGGGCT GGAGTCAATCTGCATATACCTCAGATGATTCTCATGAACACTGATTTAGGAAGCCTAGGT TGTTGGTTTTGGTCTCATCAGTAAAGGCATCCTGCCTGTGAACTCTTACAAGGAGTGAGC TTTTGCAAAATGCATGTTTATTTATGTTACAGAATGAAATATTCTATGAAATGTTCTCTA AAGCAAGTGCACAGAACAATCCCCTAAAATAGTTTGATTTCTTTACAGATCAGTTTTCTC AATGTAACATTACAGTCACATGGGAAGGCTTTAGAAAAATCTTTACCTGGGGTTCAATGA GACTAATAGCAATCTAGAGTGGGCCCAGGGAATGGTAATTATTAAAATTCAGGTGCTTCT AACAGCTGGGGCTGAGACTCCTTGCTACATAAATTTTAATACTCTTATTAGCTCTGGTTT GACTCTAGGCTTAGCAGTTTTCTAATTGAATCAGTTTCCTTGTAGTTTGCCTTCAGAGAG AATATACTGGTCTCTAAAGACTTTCTGGCTTGCTTGACTATCTTTCAACACCTGGCTTTT ATAAGAATTTCTTAGGGCAACACTAATGTAAGTTTTTTCTTTTTTGTTTTGTTTTGTTTT TTGTTTTTTTGAACCACCGAGGTCCTTTTAGTGATTGGAAGTATCAGGTTTTTAGGGATG TTACAAGGAGGAACACATACCATCCTGGGCTGCTTGGCATACAACCAAGATATGTTTTCA TATAGAACCCTTCCTTTACAGATTAGCACAAGGCCCTGTTTGGGTATTCAGCAGATCCTT CTTTATGATCTTTATTAATTTAGCAACTTGTCATTTTTTTTCACATATTGCTTTTATTTT AAAAACTTATTTGACATTCCAGAATATATGGGAAAGGAGATAATAGTTTAATAAAACCTA TGTTCCTCTCAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC012185 unedited NNNTAGTACACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGAGCCT TATAATGGTCAAAGGTTTCCAGCTAGCAGCAGTCCAGGTGACTCAAGAAAACGGCACAAG ATAAACTTCTCAAAACGTGGTCCTCCGAATCTGGCCCCACAACACATCTACTGAATGAGA ATTAGCGTCTTCCAAGTCTTAAAGTAGTCGTTTACGGACTACAGCTTGAGAAGCGCAGAA TTAGAACTTTAAGAAGTTAACGTTCTTGGATCGCAGACTAACCGAGAAGAGAGGATTCAA AAATGAAGTGATTCATCCAATCAGATTTTCCAGTAATTCACCAATCACATCGTTGGACTC TTCAGCCAATGGTTTTGTTGCGCGGGTCTTTTCAAAATCAACAAGGCCAATTAGAATGTT TCTGAGTATATATAAGGGCAGTTTCCACTCTGCCAAGTGGCTGAGTTTTTCTTTCAGGTA GTCTGAGATGGCCCGCACCAAGCAGACTGCACGCAAGTCCACCGGTGGCAAAGCGCCGCG CAAGCAGCTGGCCACTAAGGCGGCTCGGAAAAGCGCGCCGGCCACCGGCGGCGTGAAGAA ACCTCATCGCTACCGTCCCGGCACCGTGGCTCTGCGCGAGATTCGCCGCTATCAGAAGTC GACTGAGCTGCTGATCCGCAAGTTGCCTTTCCAACGCCTGGTGCGAGAAATCGCTCATGA CTTCAAGACAGATCTGCGCTTTCAGAGTTCCGCGGTGATGGCCCTGCNAGATGCCTGCGA GGCCTACTTGGTGGAGCTCTTTGAGGATACCAACCTGTGTGCCATCCATGCTAAGCGAGT GACTATCATGCCCCAAGACATTCAGCTCGCTCGCCGCATTCGTGGGGAGAGAGC
Restriction Sites	Please inquire
ACCN	BC012185
Insert Size	2429 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC012185.1
RefSeq Size	2429 bp
Locus ID	8355
Protein Pathways	Systemic lupus erythematosus
Gene Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]

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