Oct4 (POU5F1) (BC020712) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC020712 edited CATGAGTCAGTGAACAGGGAATGGGTGAATGACATTTGTGGGTAGGTTATTTCTAGAAGT TAGGTGGGCAGCTTGGAAGGCAGAGGCACTTCTACAGACTATTCCTTGGGGCCACACGTA GGTTCTTGAATCCCGAATGGAAAGGGGAGATTGATAATTGGTGTGTTTATGTTCTTACAA GTCTTCTGCCTTTTAAAATCCAGTCCCAGGACATCAAAGCTCTGCAGAAAGAACTCGAGC AATTTGCCAAGCTCCTGAAGCAGAAGAGGATCACCCTGGGATATACACAGGCCGATGTGG GGCTCACCCTGGGGGTTCTATTTGGGAAGGTATTCAGCCAAACGACCATCTGCCGCTTTG AGGCTCTGCAGCTTAGCTTCAAGAACATGTGTAAGCTGCGGCCCTTGCTGCAGAAGTGGG TGGAGGAAGCTGACAACAATGAAAATCTTCAGGAGATATGCAAAGCAGAAACCCTCGTGC AGGCCCGAAAGAGAAAGCGAACCAGTATCGAGAACCGAGTGAGAGGCAACCTGGAGAATT TGTTCCTGCAGTGCCCGAAACCGACACTGCAGCAGATCAGCCACATCGCCCAGCAGCTTG GGCTCGAGAAGGATGTGGTCCGAGTGTGGTTCTGTAACCGGCGCCAGAAGGGCAAGCGAT CAAGCAGCGACTATGCACAACGAGAGGATTTTGAGGCTGCTGGGTCTCCTTTCTCAGGGG GACCAGTGTCCTTTCCTCTGGCCCCAGGGCCCCATTTTGGTACCCCAGGCTATGGGAGCC CTCACTTCACTGCACTGTACTCCTCGGTCCCTTTCCCTGAGGGGGAAGCCTTTCCCCCTG TCTCCGTCACCACTCTGGGCTCTCCCATGCATTCAAACTGAGGTGCCTGCCCTTCTAGGA ATGGGGGACAGGGGGAGGGGAGGAGCTAGGGAAAGAAAACCTGGAGTTTGTGCCAGGGTT TTTGGGATTAAGTTCTTCATTCACTAAGGAAGGAATTGGGAACACAAAGGGTGGGGGCAG GGGAGTTTGGGGCAACTGGTTGGAGGGAAGGTGAAGTTCAATGATGCTCTTGATTTTAAT CCCACATCATGTATCACTTTTTTCTTAAATAAAGAAGCCTGGGACACAGTAGATAGGCAA AAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC020712 unedited TTCGGATTTGTATACGACNACTATAGGGCGGCCGCGAATTCGCCATTACGGCCGGGGCAT GAGTCAGTGAACAGGGAATGGGTGAATGACATTTGTGGGTAGGTTATTTCTAGAAGTTAG GTGGGCAGCTTGGAAGGCAGAGGCACTTCTACAGACTATTCCTTGGGGCCACACGTAGGT TCTTGAATCCCGAATGGAAAGGGGAGATTGATAATTGGTGTGTTTATGTTCTTACAAGTC TTCTGCCTTTTAAAATCCAGTCCCAGGACATCAAAGCTCTGCAGAAAGAACTCGAGCAAT TTGCCAAGCTCCTGAAGCAGAAGAGGATCACCCTGGGATATACACAGGCCGATGTGGGGC TCACCCTGGGGGTTCTATTTGGGAAGGTATTCAGCCAAACGACCATCTGCCGCTTTGAGG CTCTGCAGCTTAACTTCAAGAACATGTGTAAGCTGCGGCCCTTGCTGCAGAAGTGGGTGG AGGAAGCTGACAACAATGAAAATCTTCAGGAGATATGCAAAGCAGATACCCTCGTGCANG CCCGAAAGAGAAAGCGAACCAGTATCGAGAACCGAGTGAGAGGCAACCTGGAGAATTTGT TCCTGCAGTGCCCGANACCGACACTGCAGCAGATCAGCCACATCGCCCAGCAGCTTGGGC TCGAGAAGGATGTGGTCCGAGTGTGGTTCTGTAACCGGCGCCAGAAGGGCAAGCGATCAA GCAGCGACTATGCACAACGAGAGGATTTTGAGGCTGCTGGGTCTCCTTTCTCAGGGGGAC AGTGTTCCTTCTCTGGCCCAGGGCCCCATTTTTGTACCCCAGCTAGGGAAGCCTCACTGA CTGCACTGTACTCCTTGGTCCCTTTCCCTGAGGGGAAACCTTTCCCCCTGTGTCCGGACC AATCTGGGTTCTCCTGCATCAAACTGAGGGCCG
Restriction Sites	Please inquire
ACCN	BC020712
Insert Size	1167 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC020712.1
RefSeq Size	1167 bp
Locus ID	5460
Protein Families	Adult stem cells, Cancer stem cells, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors
Gene Summary	This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]

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