KRT81 (BC021241) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ghHkb1; hard keratin, type II, 1; Hb-1; HB1; hHAKB2-1; keratin, hair, basic, 1; Keratin 81; KRTHB1; MLN137
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC021241 edited GGCACGAGGGATGAAGGCCACGGTGATCAGGCACGGGGAGACCCTGCGCCGCACCAAGGA GGAGATCAACGAGCTGAACCGCATGATCCAGAGGCTGACGGCCGAGGTGGAGAATGCCAA GTGCCAGAACTCCAAGCTGGAGGCCGCGGTGGCCCAGTCTGAGCAGCAGGGTGAGGCGGC CCTCAGTGATGCCCGCTGCAAGCTGGCCGAGCTGGAGGGCGCCCTGCAGAAGGCCAAGCA GGACATGGCCTGCCTGATCAGGGAGTACCAGGAGGTGATGAACTCCAAGCTGGGCCTGGA CATCGAGATCGCCACCTACAGGCGCCTGCTGGAGGGCGAGGAGCAGAGGCTATGTGAAGG CATTGGGGCTGTGAATGTCTGTGTCAGCAGCTCCCGGGGCGGGGTCGTGTGCGGGGACCT CTGCGTGTCAGGCTCCCGGCCAGTGACTGGCAGTGTCTGCAGCGCTCCGTGCAACGGGAA CGTGGCGGTGAGCACCGGCCTGTGTGCGCCCTGCGGCCAATTGAACACCACCTGCGGAGG GGGTTCCTGCGGCGTGGGCTCCTGTGGTATCAGCTCCCTGGGTGTGGGGTCTTGCGGCAG CAGCTGCCGGAAATGTTAGGCACCCCAACTCAAGTCCCAGGCCCCAGGCGTCTTTCCTGC CCTGCCTTGCTTGGCCCATCCAGTCCAGGCGCCTGGAGCAAGTGCTCAGCTACTTCTCCT CCACTTTGAAAGACCCCTCCCACTCCTGGCCTCACATTTCTCTGTGTGATCCCCCACTTC TGGGCTCTGCCACCCCACAGTGGGAAAGGCCACCCTAGAAAGAAGTCCGCTGGCACCCAT AGGAAGGGGCCTCAGGAGCAGGAAGGGCCAGGACCAGAACCTTGCCCACGGCAACTGCCT TCCTGCCTCTCCCCTTCCTCCTCTGCTCTTGATCTGTGTTTCAATAAATTAATGTAGCCA AAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC021241 unedited GTCAAAATTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGATGAAAGCCA CGGTGATCAAGCACGGGGAGACCCTGCGCCGCACCAAGGAGGAGAACAACGAGCTGAACC GCATGATCCAGAGGCTGACGGCCGAGGTGGAGAATGCCAAGTGCCAGAACTCCAAGCTGG AGGCCGCGGTGGCCCAGTCTGAGCAGCAGGGTGAGGCGGCCCTCAGTGATGCCCGCTGCA AGCTGGCCGAGCTGGAGGGCGCCCTGCAGAAGGCCAAGCAGGACATGGCCTGCCTGATCA GGGAGTACCAGGAGGTGATGAACTCCAAGCTGGGCCTGGACATCGAGATCGCCACCTACA GGCGCCTGCTGGAGGGCGAGGAGCAGAGGCTATGTGAAGGCATTGGGGCTGTGAATGTCT GTGTCAGCAGCTCCCGGGGCGGGGTCGTGTGCGGGGACCTCTGCGTGTCAGGCTCCCGGC CAGTGACTGGCAGTGTCTGCAGCGCTCCGTGCAACGGGAACGTGGCGGTGAGCACCGGCC TGTGTGCGCCCTGCGGCCAATTGAACACCACCTGCGGAGGGGGTTCCTGCGGCGTGGGCT CCTGTGGTATCAGCTCCCTGGGTGTGGGGTCTTGCGGCAGCAGCTGCCGGAAATGTTAGG CACCCCAACTCAAGTCCCAGGCCCCAGGCGTCTTTCCTGCCCTGCCTTGCTTGGCCCATC CAGTCCAGGCGCCTGGAGCAAGTGCTCAGCTACTTCTCCTCCACTTTGAAAGACCCCTCC CACTCCTGGCCTCACATTTCTCTGTGTGATCCCCCACTTCTGGGCTCTGCCACCCCACAG TGGGAAAGGCCACCCTAGAAAGAAGTCCCGCTGCACCCCATAGAAGGGGGCCTCNAGAGC AGGAAGGGCCAGNACCAGAACCTTTGCCACGGCACTGNCTTNCTGNCTT
Restriction Sites	Please inquire
ACCN	BC021241
Insert Size	985 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021241.1, AAH21241.1
RefSeq Size	985 bp
Locus ID	3887
Gene Summary	The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB3 and KRTHB6, is found primarily in the hair cortex. Mutations in this gene and KRTHB6 have been observed in patients with a rare dominant hair disease, monilethrix. [provided by RefSeq, Jul 2008]

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