HuC (ELAVL3) (BC014144) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HUC; HUCL; PLE21
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC014144 edited AACACACGGGACCGGACAGGACCGACCGAGGGGCGGCGGCGAAAGGCAGAGCGCCGCGAT CTCTGTCGGGAAGCGCAACCTCCCCGGGCCCCGCGGGGCCGCGCAGGGGGCGTCCTCAAC CCCGCGCCCGCTCCCTCTTTCCATCCCCTGCCGCCCGCAGGCCACCCCGGGGCCCGGCCA TCCGCGCGCGCATCCCCGGGTTCGGGCCCGTCCCTGGCCCTCGAGGGAGCCGCCGCCTTC ATCGCCACATCTGCAGCGGCCGCACCAGAGGCCGCCCGGGCGGGACCCCAGCGTGAGCAT CGGGCGCCCCCCTAGGAGTGCACCACCCCCGGAGCCCCCCTCAACACGGACCGCGCCCGC CGGGCACACAAGAATGGTCACTCAGATACTGGGGGCCATGGAGTCTCAGGTGGGGGGGGG CCCGGCCGGCCCGGCCCTGCCCAACGGGCCACTCCTTGGTACAAATGGAGCCACTGACGA CAGCAAGACCAACCTCATCGTCAACTACCTGCCCCAGAACATGACCCAGGATGAGTTCAA GAGTCTCTTCGGCAGCATTGGCGACATCGAGTCCTGCAAGTTGGTTCGGGACAAGATCAC AGGGCAGAGCCTTGGCTACGGGTTTGTGAACTATTCTGACCCCAATGATGCAGACAAAGC CATCAACACCCTCAACGGCCTCAAATTACAGACGAAGACCATCAAGGTGTCCTATGCCAG ACCCAGTTCAGCATCCATCCGGGATGCTAACCTGTACGTCAGCGGGCTCCCCAAGACCAT GAGCCAGAAAGAGATGGAGCAGCTCTTCTCCCAGTACGGCCGCATCATCACGTCCCGCAT CCTGGTGGACCAGGTCACAGGTGTCTCTCGGGGTGTGGGATTCATCCGCTTTGACAAGAG GATTGAGGCCGAAGAGGCTATCAAAGGACTGAATGGGCAGAAGCCGCTGGGCGCAGCTGA GCCCATCACAGTCAAGTTCGCGAACAACCCAAGTCAGAAGACGGGGCAGGCGCTGCTCAC CCACCTCTACCAGTCATCCGCCCGGCGCTACGCAGGCCCCCTACACCATCAGACCCAGCG TTTCCGGCTGGACAATTTGCTCAACATGGCCTACGGCGTCAAGAGTCCCCTGTCGCTCAT CGCCAGGTTCTCGCCGATCGCCATCGATGGTATGAGCGGCCTGGCGGGCGTGGGCCTGTC GGGGGGCGCGGCGGGCGCCGGCTGGTGCATCTTCGTGTACAACCTGTCACCGGAGGCAGA CGAGAGCGTGCTGTGGCAGCTGTTCGGGCCTTTTGGGGCAGTCACCAACGTCAAGGTCAT CCGTGATTTCACCACCAACAAGTGCAAGGGTTTCGGCTTCGTGACCATGACCAACTATGA CGAGGCGGCCATGGCCATCGCCAGCCTGAACGGCTATCGCCTGGGCGAGCGCGTGCTGCA GGTCTCCTTCAAGACCAGCAAACAGCACAAGGCGTGAGCCCACCCCGCCTGCCCTCCCAC CCCCAAGACTGGGAGGGGACTTCTTTTTCTAAAACACAAAACTCAGCCCAGCCAGGCCCC CTCCCTGGAGGCCAGCCCCTCCCCGCAGGGAGCCCTCGCACCGCTCCCCACCCCACTCAG CGGCCCCATCGGAACTGAGAATTGCAAAACCCCCGACTTTAGCTATAGTTAAGTTGCCTT CCCCCGGTGTCCCCACGTTTGGTGTCTGGCTATGGCGCCCCCATCTGACTGTCCAGCTCT CTCTCCCCCACCCCTCCCGGTGTTGTTATTAAACGTCTGTGGAGCTTCTGCTGCAAGGAA CAAAAAAGAAAAAAATCAAAAAAGCGACAAAAAAACACAAACAGAAGAGGAAAAAAAAGC AACAAAAAAAGAAAACACACAGAAGAGATTTAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC014144 unedited GGAAGCGTCAGAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGTAGNAAC ACACGGGTACCGGTACAGGACCGACCGAGGGGCGGCGGCGAAAGGCAGAGCGCCGCGATC TCTGTCGGGAAGCGCAACCTCCCCGGGCCCCGCGGGGCCGCGCAGGGGGCGTCCTCAACC CCGCGCCCGCTCCCTCTTTCCATCCCCTGCCGCCCGCAGGCCACCCCGGGGCCCGGCCAT CCGCGCGCGCATCCCCGGGTTCGGGCCCGTCCCTGGCCCTCGAGGGAGCCGCCGCCTTCA TCGCCACATCTGCAGCGGCCGCACCAGAGGCCGCCCGGGCGGGACCCCAGCGTGAGCATC GGGCGCCCCCCTAGGAGTGCACCACCCCCGGAGCCCCCCTCAACACGGACCGCGCCCGCC GGGCACACAAGAATGGTCACTCAGATACTGGGGGCCATGGAGTCTCAGGTGGGGGGGGGC CCGGCCGGCCCGGCCCTGCCCAACGGGCCACTCCTTGGTACAAATGGAGCCACTGACGAC AGCAAGACCAACCTCATCGTCAACTACCTGCCCCAGAACATGACCCAGGATGAGTTCAAG AGTCTCTTCGGCAGCATTGGCGACATCGAGTCCTGCAAGTTGGTTCGGGACAAGATCACA GGGCAGAGCCTTGGCTACGGGTTTGTGAACTATTCTGACCCCAATGATGCAGACAAAGCC ATCAACACCCTCAACGGCCTCAAATTACAGACGAAGACCATCAAGGTGTCCTATGCCAGA CCCAGTTCAGCATCCATCCGGGATGCTAACCTGTACGTCAGCGGGCTCCCAAGACCATAG CCCGAAAGAGATGGACAACTCTTCTCCAAGTACGCCGGATCATCACGTCCCGCATCCTGT TGGGACAGGTCACAGGTG
Restriction Sites	Please inquire
ACCN	BC014144
Insert Size	1922 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC014144.2
RefSeq Size	1913 bp
Locus ID	1995
Gene Summary	A member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.